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doctoral thesis
Odgovor uročnjaka (Arabidopsis thaliana (L.) Heynh.) s promijenjenom ekspresijom gena BPM i DMS3 na toplinski stres
Zagreb: University of Zagreb, Faculty of Science, 2024. urn:nbn:hr:217:666656
Vitko, Sandra
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Vitko, S. (2024). Odgovor uročnjaka (Arabidopsis thaliana (L.) Heynh.) s promijenjenom ekspresijom gena BPM i DMS3 na toplinski stres (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:666656

Vitko, Sandra. "Odgovor uročnjaka (Arabidopsis thaliana (L.) Heynh.) s promijenjenom ekspresijom gena BPM i DMS3 na toplinski stres." Doctoral thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:666656

Vitko, Sandra. "Odgovor uročnjaka (Arabidopsis thaliana (L.) Heynh.) s promijenjenom ekspresijom gena BPM i DMS3 na toplinski stres." Doctoral thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:666656

Vitko, S. (2024). 'Odgovor uročnjaka (Arabidopsis thaliana (L.) Heynh.) s promijenjenom ekspresijom gena BPM i DMS3 na toplinski stres', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 26 June 2024, https://urn.nsk.hr/urn:nbn:hr:217:666656

Vitko S. Odgovor uročnjaka (Arabidopsis thaliana (L.) Heynh.) s promijenjenom ekspresijom gena BPM i DMS3 na toplinski stres [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2024 [cited 2024 June 26] Available at: https://urn.nsk.hr/urn:nbn:hr:217:666656

S. Vitko, "Odgovor uročnjaka (Arabidopsis thaliana (L.) Heynh.) s promijenjenom ekspresijom gena BPM i DMS3 na toplinski stres", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:217:666656

Metadata
TitleOdgovor uročnjaka (Arabidopsis thaliana (L.) Heynh.) s promijenjenom ekspresijom gena BPM i DMS3 na toplinski stres
Title (english)Response of Arabidopsis thaliana (L.) Heynh. with modified BPM and DMS3 gene expression to heat stress
AuthorSandra Vitko
MentorŽeljka Vidaković-Cifrek (mentor)
Committee memberMirta Tkalec (predsjednik povjerenstva)
Committee memberNataša Bauer (član povjerenstva)
Committee memberTatjana Prebeg (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2024, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
Proteini BPM i DMS3 sudjeluju u regulaciji rasta i razvoja te prilagodbi biljaka na okolišne uvjete. Učinak toplinskog tretmana istražen je u biljaka uročnjaka s prekomjernom (oeBPM1 i oeDMS3) i smanjenom (amiR-bpm i dms3-1) ekspresijom tih proteina te biljaka divljeg tipa. Fenotipizacijom je dokazan smanjeni rast linija oeBPM1 i dms3-1 u kontrolnim uvjetima, a toplinski tretman uzrokovao je inhibiciju rasta u divljeg tipa te linija oeDMS3, oeBPM1 i amiR-bpm. U liniji dms3-1 tretiranoj u kasnijem razvojnom stadiju zabilježena je povećana stopa rasta. Sve linije tretirane u stadiju klijanaca akumulirale su protein HSP90, no indukcija HSP70 ovisila je o genskoj pozadini biljaka. U linijama oeBPM1 i amiR-bpm primijećeni su oprečni odgovori na stres – u prvoj liniji je zabilježena smanjena fotosintetska učinkovitost, a u drugoj povišena aktivnost antioksidacijskih enzima. U liniji oeDMS3 izmjerena je povišena aktivnost peroksidaza, a linija dms3-1 pokazala je veću osjetljivost fotosinteze unatoč povećanju sadržaja prolina i aktivnosti katalaze. Dobiveni rezultati ukazuju da promijenjena ekspresija gena BPM nema jednoznačnu ulogu u termotoleranciji, ali da je za otpornost u stadiju klijanaca potrebna prisutnost funkcionalnog proteina DMS3.
Abstract (english)
Proteins BPM and DMS3 participate in the regulation of growth and development, and adaptation of plants to environmental conditions. The effects of heat treatment were studied in Arabidopsis thaliana plants with overexpression (oeBPM1 and oeDMS3) and downregulation (amiR-bpm and dms3-1) of these proteins, as well as in wild-type plants. Phenotyping of plants showed reduced growth of oeBPM1 and dms3-1 lines under control conditions, while heat treatment caused growth inhibition of wild type and oeDMS3, oeBPM1 and amiR-bpm lines. The dms3-1 line treated at a later developmental stage showed an increased growth rate. All lines treated at the seedling stage accumulated HSP90 protein, but the induction of HSP70 depended on the genetic background of the plants. In oeBPM1 and amiR-bpm lines, opposite responses to stress were observed – reduced photosynthetic efficiency in the former and increased antioxidant enzyme activity in the latter. Increased peroxidase activity was measured in oeDMS3 line, while dms3-1 line showed photosynthetic sensitivity despite higher proline content and catalase activity. The results suggest that the altered expression of BPM genes does not have a straightforward role in thermotolerance, but the presence of a functional DMS3 protein is essential for tolerance in the seedling stage.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:666656
Promotion2024
ProjectNumber: IP-2016-06-6229
Title: MATH-BTB proteini kao regulatori transkripcije i RNA posredovane metilacije DNA u biljnom razvitku
Acronym: PHYTOMETHDEV
Leader: Dunja Leljak-Levanić
Jurisdiction: Croatia
Funder: Hrvatska zaklada za znanost
Funding stream: Research Projects
ProjectNumber: 284443
Title: European Plant Phenotyping Network
Acronym: EPPN
Leader: Željka Vidaković Cifrek
Jurisdiction: eu
Funder: EC
Funding stream: FP7
Study programmeTitle: Doctoral study
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-05-07 08:58:31