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master's thesis
Estrogenski ili anti-estrogenski učinci ekstrakata tradicionalno korištenih tropskih biljaka na ljudske stanice U2OS
Zagreb: University of Zagreb, Faculty of Science, 2016. urn:nbn:hr:217:230678
Zaplatić, Elizabeta
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Zaplatić, E. (2016). Estrogenski ili anti-estrogenski učinci ekstrakata tradicionalno korištenih tropskih biljaka na ljudske stanice U2OS (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:230678

Zaplatić, Elizabeta. "Estrogenski ili anti-estrogenski učinci ekstrakata tradicionalno korištenih tropskih biljaka na ljudske stanice U2OS." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:230678

Zaplatić, Elizabeta. "Estrogenski ili anti-estrogenski učinci ekstrakata tradicionalno korištenih tropskih biljaka na ljudske stanice U2OS." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:230678

Zaplatić, E. (2016). 'Estrogenski ili anti-estrogenski učinci ekstrakata tradicionalno korištenih tropskih biljaka na ljudske stanice U2OS', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:230678

Zaplatić E. Estrogenski ili anti-estrogenski učinci ekstrakata tradicionalno korištenih tropskih biljaka na ljudske stanice U2OS [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2016 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:230678

E. Zaplatić, "Estrogenski ili anti-estrogenski učinci ekstrakata tradicionalno korištenih tropskih biljaka na ljudske stanice U2OS", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:217:230678

Metadata
TitleEstrogenski ili anti-estrogenski učinci ekstrakata tradicionalno korištenih tropskih biljaka na ljudske stanice U2OS
Title (english)Estrogenic or anti-estrogenic effects of traditionally used tropical plant extracts on human U2OS cells
AuthorElizabeta Zaplatić
MentorGünter Vollmer (mentor)
MentorInga Marijanović (mentor)
Committee memberInga Marijanović (predsjednik povjerenstva)
Committee memberAntun Alegro (član povjerenstva)
Committee memberDomagoj Đikić (član povjerenstva)
Committee memberVesna Benković (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2016-02-22, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Fitoestrogeni su spojevi biljnog porijekla koji, zahvaljujući strukturnoj sličnosti sa 17β-estradiolom, posjeduju mogućnost vezanja na estrogenske receptore te mogu djelovati kao njihovi agonisti i/ili antagonisti. Fitoestrogenima se pripisuju brojni zdravstveni benefiti povezani sa kardiovaskularnim bolestima, hormonski ovisnim tumorima, osteoporozom i menopauzalnim simptomima. Tropska Afrička biljka, Mondia whitei (Hook.f.) Skeels, koristi se u tradicionalnoj medicini za liječenje raznih bolesti, no njezina upotreba najčešće se spominje vezano za spolne probleme muškaraca. To ukazuje na potencijalni mehanizam djelovanja povezan sa hormonima, te su u ovom radu ispitana estrogenska i anti-estrogenska svojstva metanolskog ekstrakta Mondie whitei i njegovih frakcija. Istraživanje je uključivalo serije transaktivacijskih testova u U2OS-ERα i U2OS-ERβ stanicama. U ispitanom metanolnskom ekstraktu nije pronađena niti estrogenska niti anti-estrogenska aktivnost. Jedna frakcija ekstrakta pokazala je agonističku aktivnost u obje stanične linije, dok antiestrogenska aktivnost nije zabilježena niti u jednoj frakciji. Također, ispitana je i toksičnost biljnog ekstrakta i frakcija, no zbog nekih neočekivanih opaženih efekata, savjetuje se ponoviti MTT test. Ovaj rad izrađen je u sklopu većeg “Angola Projekta” čiji je cilj dokazati i znanstveno utemeljiti učinke biljaka korištenih u tradicionalnoj medicini.
Abstract (english)
Phytoestrogens are plant-derived compounds that, due to their structural similarity with 17β- estradiol, have an ability to bind to estrogen receptors and act as their agonists or/and antagonists. They have been reported to confer health benefits related to cardiovascular diseases, hormonedependent cancers, osteoporosis, and menopausal symptoms. A tropical African plant, Mondia whitei (Hook.f.) Skeels, is used as a traditional remedy to treat various conditions and diseases, but its use is most commonly reported in the terms of male sexual problems. This indicates potential hormonal-related mechanism of action, so this study evaluated estrogenic and anti-estrogenic properties of methanol extract of Mondia whitei and its fractions. Herbal extract and fractions were tested using estrogen receptor subtype specific transactivation assays in U2OS-ERα and U2OS-ERβ cells. In the examined methanol extract, neither estrogenic nor anti-estrogenic properties have been observed. One fraction of the extract showed agonistic activity in both cell lines, whereas none of the fractions showed anti-estrogenic potential. In addition to that, applied herbal treatments were examined for cytotoxicity, but due to some confounding effects observed, MTT assay is advised to be repeated. This study was conducted as part of the bigger project, “Angola Project”, which aims to a better, science based understanding of traditionally known medicinal uses of plants.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:230678
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-05-12 12:12:43