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doctoral thesis
ULOGA TNF-α I IFN-γ U INTERAKCIJAMA TUMORSKIH STANICA I STANICA TUMORSKOG MIKROOKOLIŠA U PRIMARNOM MEDIJASTINALNOM B-STANIČNOM LIMFOMU
Zagreb: University of Zagreb, Faculty of Science, 2024. urn:nbn:hr:217:667323
Gršković, Paula
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Gršković, P. (2024). ULOGA TNF-α I IFN-γ U INTERAKCIJAMA TUMORSKIH STANICA I STANICA TUMORSKOG MIKROOKOLIŠA U PRIMARNOM MEDIJASTINALNOM B-STANIČNOM LIMFOMU (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:667323

Gršković, Paula. "ULOGA TNF-α I IFN-γ U INTERAKCIJAMA TUMORSKIH STANICA I STANICA TUMORSKOG MIKROOKOLIŠA U PRIMARNOM MEDIJASTINALNOM B-STANIČNOM LIMFOMU." Doctoral thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:667323

Gršković, Paula. "ULOGA TNF-α I IFN-γ U INTERAKCIJAMA TUMORSKIH STANICA I STANICA TUMORSKOG MIKROOKOLIŠA U PRIMARNOM MEDIJASTINALNOM B-STANIČNOM LIMFOMU." Doctoral thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:667323

Gršković, P. (2024). 'ULOGA TNF-α I IFN-γ U INTERAKCIJAMA TUMORSKIH STANICA I STANICA TUMORSKOG MIKROOKOLIŠA U PRIMARNOM MEDIJASTINALNOM B-STANIČNOM LIMFOMU', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 23 July 2024, https://urn.nsk.hr/urn:nbn:hr:217:667323

Gršković P. ULOGA TNF-α I IFN-γ U INTERAKCIJAMA TUMORSKIH STANICA I STANICA TUMORSKOG MIKROOKOLIŠA U PRIMARNOM MEDIJASTINALNOM B-STANIČNOM LIMFOMU [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2024 [cited 2024 July 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:667323

P. Gršković, "ULOGA TNF-α I IFN-γ U INTERAKCIJAMA TUMORSKIH STANICA I STANICA TUMORSKOG MIKROOKOLIŠA U PRIMARNOM MEDIJASTINALNOM B-STANIČNOM LIMFOMU", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:217:667323

Metadata
TitleULOGA TNF-α I IFN-γ U INTERAKCIJAMA TUMORSKIH STANICA I STANICA TUMORSKOG MIKROOKOLIŠA U PRIMARNOM MEDIJASTINALNOM B-STANIČNOM LIMFOMU
Title (english)THE ROLE OF TNF-α AND IFN-γ IN THE INTERACTIONS BETWEEN TUMOUR CELLS AND TUMOUR MICROENVIRONMENT CELLS IN PRIMARY MEDIASTINAL B CELL LYMPHOMA
AuthorPaula Gršković
MentorPetra Korać (mentor)
Committee memberInga Urlić (predsjednik povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2024-05-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
Primarni medijastinalni B-stanični limfom (PMBCL) je agresivni tip nehodgkinskog limfoma (NHL). Dijeli karakteristike s difuznim B-velikostaničnim limfomom (DLBCL), ali i s klasičnim Hodgkinovim limfomom (cHL), uključujući aberacije gena uključenih u regulaciju imunosnog odgovora. U ovom istraživanju ekspresija gena TNF-α, IFN-γ, cREL, CXCL10, CXCR3, PD-L1 i PD-1 u uzorcima tkiva PMBCL-a bila je uspoređena s njihovom ekspresijom u netumorskim limfocitima B i T metodom lančane reakcije polimerazom u stvarnom vremenu (qPCR). Modelne stanične linije PMBCL-a Farage, U2940, MedB-1 i Karpas1106p bile su tretirane s različitim koncentracijama TNF-α i IFN-γ tijekom 24 i 48 sati, a zatim se kvantificirala ekspresija gena cREL, CXCL10 i PD-L1 metodom qPCR. Dulji tretman s TNF-α uzrokovao je povišenje ekspresije cREL, što bi moglo utjecati na ekspresiju nizvodnih gena. Tretman s IFN-γ potaknuo je visoku ekspresiju CXCL10, koja je potencijalno bila niska u uzorcima tumora pacijenata i netretiranim staničnim linijama zbog niske ekspresije IFN-γ. Ekspresija PD-L1 nije pokazala ujednačen odgovor na tretmane u različitim staničnim linijama. Ovi rezultati sugeriraju da tumorske stanice stvaraju upalni mikrookoliš i reguliraju kemotaksiju limfocita T kako bi izbjegle imunosni odgovor.
Abstract (english)
Primary mediastinal B-cell lymphoma (PMBCL) is an aggressive type of non-Hodgkin lymphoma (NHL). It shares features with diffuse large B-cell lymphoma (DLBCL), but also with classical Hodgkin lymphoma (cHL), including aberrations of genes involved in the regulation of immune response. In this study, the expression of genes TNF-α, IFN-γ, cREL, CXCL10, CXCR3, PD-L1 and PD-1 was compared between PMBCL tissue samples and non-tumour B- and T-cells using quantitative polymerase chain reaction (qPCR). PMBCL model cell lines Farage, U2940, MedB-1 and Karpas1106p were treated with different concentrations of TNF-α and IFN-γ over 24 and 48 hours, which was followed by the quantification of cREL, CXCL10 and PD-L1 expression using qPCR. Prolonged exposure to TNF-α increased the expression of cREL, and possibly the expression of downstream genes. IFN-γ strongly induced CXCL10 expression, which seemed to be inhibited in non-treated PMBCL cell lines and patients’ tissue samples. The expression of PD-L1 did not display uniform response to the treatments across various cell lines. These results suggest that PMBCL tumour cells create proinflammatory microenvironment and regulate T-cell chemotaxis in order to avoid immune response.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:667323
Promotion2024
Study programmeTitle: Doctoral study
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-06-04 08:58:59