prikaz prve stranice dokumenta Usporedba metoda ImmunoCAP i IMMULITE za mjerenje imunoglobulina E te korelacija s rezultatima kožnog ubodnog testa
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master's thesis
Usporedba metoda ImmunoCAP i IMMULITE za mjerenje imunoglobulina E te korelacija s rezultatima kožnog ubodnog testa
Zagreb: University of Zagreb, Faculty of Science, 2016. urn:nbn:hr:217:931094
Knezović, Anela
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Knezović, A. (2016). Usporedba metoda ImmunoCAP i IMMULITE za mjerenje imunoglobulina E te korelacija s rezultatima kožnog ubodnog testa (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:931094

Knezović, Anela. "Usporedba metoda ImmunoCAP i IMMULITE za mjerenje imunoglobulina E te korelacija s rezultatima kožnog ubodnog testa." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:931094

Knezović, Anela. "Usporedba metoda ImmunoCAP i IMMULITE za mjerenje imunoglobulina E te korelacija s rezultatima kožnog ubodnog testa." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:931094

Knezović, A. (2016). 'Usporedba metoda ImmunoCAP i IMMULITE za mjerenje imunoglobulina E te korelacija s rezultatima kožnog ubodnog testa', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:931094

Knezović A. Usporedba metoda ImmunoCAP i IMMULITE za mjerenje imunoglobulina E te korelacija s rezultatima kožnog ubodnog testa [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2016 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:931094

A. Knezović, "Usporedba metoda ImmunoCAP i IMMULITE za mjerenje imunoglobulina E te korelacija s rezultatima kožnog ubodnog testa", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:217:931094

Metadata
TitleUsporedba metoda ImmunoCAP i IMMULITE za mjerenje imunoglobulina E te korelacija s rezultatima kožnog ubodnog testa
Title (english)Intermethod comparison of detection performance for allergen-specific IgE antibodies between ImmunoCAP and IMMULITE and agreement with skin prick testing
AuthorAnela Knezović
MentorMirjana Turkalj (mentor)
MentorNada Oršolić (mentor)
Committee memberNada Oršolić (predsjednik povjerenstva)
Committee memberSandra Radić Brkanac (član povjerenstva)
Committee memberRenata Matoničkin Kepčija (član povjerenstva)
Committee memberAna Galov (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2016-02-29, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Učestalost alergijskih bolesti u posljednjih 20-30 godina u dramatičnom je porastu, posebno kod djece. Razlog trenda porasta još uvijek nije poznat. Poznato je da alergijska bolest ima nekoliko faza: senzibilizaciju, manifestaciju simptoma, perzistenciju i remisiju. Moderna medicina teži prevenciji, sprječavanju progresije bolesti i dijagnosticiranju bolesti u najranijim fazama. Ključni dio dijagnostike alergijskih bolesti je otkrivanje alergena koji izazivaju reakciju. Danas se dijagnostika alergijskih bolesti provodi in vivo i in vitro testovima. Osnovni in vivo test je kožni ubodni test koji mjeri kožnu reakciju na specifični alergen apliciran u kožu. Osnovni in vitro testovi mjere razinu specifičnog imunoglobulina E u uzorku seruma. Cilj ovog istraživanja bio je usporediti rezultate mjerenja specifičnih IgE protutijela mjerenih metodama ImmunoCAP i IMMULITE, te rezultate usporediti s rezultatima kožnog ubodnog testa na iste alergene. Obradom uzoraka iz selektirane i neselektirane skupine ispitanika uspoređene su navedene metode. Rezultati su pokazali da su ImmunoCAP i IMMULITE metode usporedive i približno jednako osjetljive. Metoda IMMULITE pokazala je viši stupanj slaganja s rezultatima kožnog testa. Obje metode su valjane u rutinskoj dijagnostici alergijskih bolesti, a niti jedna metoda nije dostatna niti idealna u dijagnostici alergijskih bolesti.
Abstract (english)
Prevalence of allergic diseases has been in dramatic increase over last 20-30 years, especially in children. The reason of this increasing trend is still unknown. It's known that allergic disease has several phases: sensibilisation, symptoms manifestation, persistence and remision. Modern medicine tends to prevent, to preclude progression of the disease and to diagnose disease in the earliest phases. A key part of diagnosing allergic diseases is detection of allergen that causes reaction. Today, diagnostics of allergic diseases is carried out by in vivo and in vitro tests. General in vivo test is a skin prick test that measures skin reactions at specific allergen injected into the skin. General in vitro tests measures the level of specific IgE in a sera sample. The goal of this research was to compare the results of measurements of specific IgE antibodies measured by ImmunoCAP and IMMULITE methods, and to compare that results with the results of skin prick test on the same allergens. We compared mentioned methods processing selected and nonselected group of samples. The results showed that IMMULITE and ImmunoCAP are comparable and approximately equally sensitive methods. The IMMULITE showed better correlation with the skin prick test results. Both methods are valid in the routine diagnostics of allergic diseases, but no method is sufficient nor ideal in diagnostics of allergic diseases.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:931094
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-05-12 12:48:54