prikaz prve stranice dokumenta Fenolne tvari iz obične planike (Arbutus unedo L.) i njihovi biološki učinci
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doctoral thesis
Fenolne tvari iz obične planike (Arbutus unedo L.) i njihovi biološki učinci
Zagreb: University of Zagreb, Faculty of Science, 2016. urn:nbn:hr:217:488435
Jurica, Karlo
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Jurica, K. (2016). Fenolne tvari iz obične planike (Arbutus unedo L.) i njihovi biološki učinci (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:488435

Jurica, Karlo. "Fenolne tvari iz obične planike (Arbutus unedo L.) i njihovi biološki učinci." Doctoral thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:488435

Jurica, Karlo. "Fenolne tvari iz obične planike (Arbutus unedo L.) i njihovi biološki učinci." Doctoral thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:488435

Jurica, K. (2016). 'Fenolne tvari iz obične planike (Arbutus unedo L.) i njihovi biološki učinci', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:488435

Jurica K. Fenolne tvari iz obične planike (Arbutus unedo L.) i njihovi biološki učinci [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2016 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:488435

K. Jurica, "Fenolne tvari iz obične planike (Arbutus unedo L.) i njihovi biološki učinci", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:217:488435

Metadata
TitleFenolne tvari iz obične planike (Arbutus unedo L.) i njihovi biološki učinci
Title (english)Phenolic compounds in strawberry tree (Arbutus unedo L.) and their biological effects
AuthorKarlo Jurica
MentorDario Kremer (mentor)
MBZ: 240541
MentorVesna Benković (sumentor)
Committee memberDomagoj Đikić (član povjerenstva)
Committee memberSandra Radić Brkanac (član povjerenstva)
MBZ: 245550
Committee memberNevenka Kopjar (član povjerenstva)
MBZ: 195102
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2016, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
U ovom su radu ispitane fitokemijske značajke, antimikrobna svojstva i biološki učinci lista obične planike. Određen je sadržaj ukupnih fenola, flavonoida, tanina, fenolnih kiselina, te polifenolni profil lista UHPLC-MS/MS (Orbitrap) metodom, sadržaj arbutina i hidrokinona HPLC-UV/DAD metodom, antioksidacijska svojstva FRAP, TEAC i DPPH metodama. MIK koncentracije su utvrđene dvostrukom mikrodilucijom, a mutagenost komet-testom i CBMN testom. Dokazano je 37 novih fenolnih spojeva. Sadržaj arbutina u listu bio je 0,3 %, dok hidrokinon nije detektiran. Metanol je bolje sredstvo za ekstrakciju polifenola od vode. Metanolni ekstrakt lista pokazao je bolji antioksidacijski, te antimikrobni učinak na ATCC i kliničke sojeve mikroorganizama. Arbutin nije pokazao antimikrobni učinak, dok je njegov metabolit hidrokinon pokazao pozitivan učinak na ispitivane uropatogene. U uvjetima in vitro i in vivo vodeni ekstrakt lista pri koncentraciji arbutina koja odgovara maksimalno dopuštenom dnevnom unosu ima nisku genotoksičnost. Potencijal vodenog ekstrakta lista obične planike kao fitoterpeutika za kliničku primjenu je velik.
Abstract (english)
This thesis investigated the phytochemical characteristics, antimicrobial properties and biological effects of strawberry tree leaves. Total phenol, tannin, flavonoid and phenolic acid content was determined as well as polyphenolic profile using the UHPLC-MS/MS Orbitrap method. Arbutin and hydroquinone concentrations were measured by HPLC-UV/DAD, antioxidative properties by FRAP, TEAC, DPPH, MIC concentrations by a Twofolded Microdilution method and mutagenicity by Comet-test and CBMN test. Thirty-seven new phenolic compounds were detected. Leaves contained 0.3% of arbutin while hydroquinone was not detected. Methanol was shown to be a more effective solvent for the extraction of polyphenols. Methanolic extract showed better antioxidative properties and better antimicrobial effect on standard ATCC and clinical microorganism strains. Arbutin did not show antimicrobial activity, while its metabolite hydroquinone showed positive antimicrobial activity against the tested uropathogens. Water extract applied at an arbutin concentration corresponding to the maximum allowable daily intake showed low genotoxicity in vitro and in vivo. Strawberry tree water leaf extract has great potential as a phytotherapeutic in clinical application.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:488435
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-16 08:45:52