prikaz prve stranice dokumenta Analiza degradacije lipoglikodepsipeptidnog antibiotika ramoplanina tekućinskom kromatografijom i spektrometrijom masa
Embargo period 2026-06-13
doctoral thesis
Analiza degradacije lipoglikodepsipeptidnog antibiotika ramoplanina tekućinskom kromatografijom i spektrometrijom masa
Zagreb: University of Zagreb, Faculty of Science, 2024. urn:nbn:hr:217:270151
Stražić Novaković, Dubravka
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Stražić Novaković, D. (2024). Analiza degradacije lipoglikodepsipeptidnog antibiotika ramoplanina tekućinskom kromatografijom i spektrometrijom masa (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:270151

Stražić Novaković, Dubravka. "Analiza degradacije lipoglikodepsipeptidnog antibiotika ramoplanina tekućinskom kromatografijom i spektrometrijom masa." Doctoral thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:270151

Stražić Novaković, Dubravka. "Analiza degradacije lipoglikodepsipeptidnog antibiotika ramoplanina tekućinskom kromatografijom i spektrometrijom masa." Doctoral thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:270151

Stražić Novaković, D. (2024). 'Analiza degradacije lipoglikodepsipeptidnog antibiotika ramoplanina tekućinskom kromatografijom i spektrometrijom masa', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:270151

Stražić Novaković D. Analiza degradacije lipoglikodepsipeptidnog antibiotika ramoplanina tekućinskom kromatografijom i spektrometrijom masa [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2024 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:217:270151

D. Stražić Novaković, "Analiza degradacije lipoglikodepsipeptidnog antibiotika ramoplanina tekućinskom kromatografijom i spektrometrijom masa", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:217:270151

Metadata
TitleAnaliza degradacije lipoglikodepsipeptidnog antibiotika ramoplanina tekućinskom kromatografijom i spektrometrijom masa
Title (english)Degradation study of lipoglycodepsipeptide antibiotic ramoplanin by liquid chromatography and mass spectrometry
AuthorDubravka Stražić Novaković
MentorNives Galić (mentor)
Committee memberIva Juranović Cindrić (predsjednik povjerenstva)
Committee memberŠime Ukić (član povjerenstva)
Committee memberInes Primožič (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2024-06-12, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Universal decimal classification
(UDC)		54 - Chemistry. Crystallography. Mineralogy
Abstract
U borbi protiv bakterijske rezistencije, ciklički lipoglikodepsipeptid ramoplanin pojavio se kao novi obećavajući antibiotski lijek. U ovom radu je razvijena, validirana i upotrjebljena metoda tekućinske kromatografije za praćenje stabilnosti ramoplanina, u čvrstom stanju i u otopini. Studija forsirane razgradnje provedena je podvrgavanjem uzorka različitim stresnim uvjetima (kisela i bazična hidroliza, oksidacija, vlaga, temperatura i svjetlo) te su opaženi razgradni produkti razjašnjeni spektrometrijom masa. Istraživanje je otkrilo kako je hidroliza laktonskog prstena najvažniji degradacijski put molekule. Međutim, opaženi su i drugi značajni putevi degradacije. Nadalje, ramoplanin je formuliran u vodenim otopinama koristeći nekoliko različitih pomoćnih tvari te je provedena stabilitetna studija pri 25 °C/60 % RV i 40 °C/75 % RV. Među ispitanim pomoćnim tvarima, polisorbat 20, Kolliphor® RH 40 i kolin klorid pokazali su važan stabilizacijski učinak na degradaciju ramoplanina.
Abstract (english)
In the fight against antimicrobial resistance, a cyclic lipoglycodepsipeptide ramoplanin emerged as a new promising antibacterial regimen. In the present work, an LC method was developed, validated and used during stability testing of ramoplanin, in solid state and in solution. Forced degradation study was performed by subjecting the sample to different stress conditions (acid and base hydrolysis, oxidation, humidity, heat and light) and the observed degradation products were elucidated using mass spectrometry. The study revealed that the most significant degradation path of the molecule is hydrolysis of its lactone ring. However, other significant degradation routs were also observed. Furthermore, ramoplanin was formulated in aqueous solutions using several different excipients and a stability study was conducted at 25 °C/60 % RH and 40 °C/75 % RH. Among the tested excipients, polysorbate 20, Kolliphor® RH 40 and choline chloride were found to have an important stabilizing effect on the degradation of ramoplanin.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:270151
Promotion2024
Study programmeTitle: Doctoral study in chemistry
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija (doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija)
Type of resourceText
Extent108, XXXIII str. ; 30 cm
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2026-06-13
Terms of useLicence In copyright icon
Created on2024-06-21 12:30:40