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master's thesis
Praćenje bolesti račje kuge u populacijama invazivne vrste bodljobradog raka (Faxonius limosus) u Hrvatskoj
Zagreb: University of Zagreb, Faculty of Science, 2024. urn:nbn:hr:217:754514
Bednjanić, Mia
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Bednjanić, M. (2024). Praćenje bolesti račje kuge u populacijama invazivne vrste bodljobradog raka (Faxonius limosus) u Hrvatskoj (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:754514

Bednjanić, Mia. "Praćenje bolesti račje kuge u populacijama invazivne vrste bodljobradog raka (Faxonius limosus) u Hrvatskoj." Master's thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:754514

Bednjanić, Mia. "Praćenje bolesti račje kuge u populacijama invazivne vrste bodljobradog raka (Faxonius limosus) u Hrvatskoj." Master's thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:754514

Bednjanić, M. (2024). 'Praćenje bolesti račje kuge u populacijama invazivne vrste bodljobradog raka (Faxonius limosus) u Hrvatskoj', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:754514

Bednjanić M. Praćenje bolesti račje kuge u populacijama invazivne vrste bodljobradog raka (Faxonius limosus) u Hrvatskoj [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2024 [cited 2024 November 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:754514

M. Bednjanić, "Praćenje bolesti račje kuge u populacijama invazivne vrste bodljobradog raka (Faxonius limosus) u Hrvatskoj", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:217:754514

Metadata
TitlePraćenje bolesti račje kuge u populacijama invazivne vrste bodljobradog raka (Faxonius limosus) u Hrvatskoj
Title (english)Monitoring of crayfish plague in populations of the invasive spiny-cheek crayfish (Faxonius limosus) in Croatia
AuthorMia Bednjanić
MentorSandra Hudina (mentor)
MentorAna Bielen (komentor)
Committee memberSandra Hudina (predsjednik povjerenstva)
Committee memberIvana Maguire (član povjerenstva)
Committee memberJasna Lajtner (član povjerenstva)
Committee memberZoran Marčić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2024-07-12, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Bodljobradi rak (Faxonius limosus) je invazivna vrsta koja predstavlja značajnu prijetnju autohtonim populacijama rakova u Europi. Potječe iz Sjeverne Amerike te se brzo širi na nova područja gdje ulazi u kompeticiju za resurse s autohtonim vrstama te prenosi bolesti poput račje kuge. Račja kuga, uzrokovana patogenom Aphanomyces astaci, ima devastirajući učinak na autohtone vrste rakova, što naglašava potrebu za stalnim monitoringom ovog patogena. Na području istočne Hrvatske, Faxonius limosus širi svoj areal u Dunavu i Dravi te ugrožava nativne vrste rakova Astacus astacus i Pontastacus leptodactylus. Dosadašnja istraživanja pokazala su prisutnost patogena u populacijama F. limosus, ali bez uspješne genotipizacije zbog niske koncentracije DNA patogena u uzorcima. Cilj ovog istraživanja bio je utvrditi prisutnost patogena Aphanomyces astaci u recetnim uzorcima bodljobradog raka iz istočne Hrvatske. Koristeći PCR metodu patogen nije detektiran u 11 analiziranih jedinki Faxonius limosus, ali je detektiran u uzorku autohtone vrste Astacus astacus sakupljene u istočnom dijelu Republike Hrvatske. Genotipizacija patogena A. astaci dobivenog iz vrste A. astacus nije bila uspješna, vjerojatno zbog preniske koncentracije DNA patogena u uzorku. U nastavku istraživanja potrebno je koristiti osjetljivije molekularne metode za pouzdaniju detekciju patogena.
Abstract (english)
The spiny-cheek crayfish (Faxonius limosus) is an invasive species that poses a significant threat to native crayfish populations in Europe. Originating from North America, it rapidly spreads to new areas, competing with native species for resources and transmitting diseases such as crayfish plague. Crayfish plague, caused by the pathogen Aphanomyces astaci, has a devastating impact on native crayfish species, highlighting the need for constant monitoring of this pathogen. In eastern Croatia, Faxonius limosus is expanding its range in the Danube and Drava rivers, threatening the native crayfish species Astacus astacus and Pontastacus leptodactylus. Previous research has shown the presence of the pathogen in populations of F. limosus, but without successful genotyping due to the low concentration of pathogen DNA in the samples. The aim of this study was to determine the presence of the pathogen Aphanomyces astaci in recent samples of spiny-cheek crayfish from eastern Croatia. Using the PCR method, the pathogen was not detected in 11 analyzed individuals of Faxonius limosus, but it was detected in a sample of the native species Astacus astacus collected in the eastern part of the Republic of Croatia. Genotyping of the A. astaci pathogen obtained from A. astacus was unsuccessful, probably due to the low concentration of pathogen DNA in the sample. Further research needs to use more sensitive molecular methods for more reliable detection of the pathogen.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:754514
Study programmeTitle: Ecology and Nature Preservation-University graduate programme
Study programme type: university
Study level: graduate
Academic / professional title: sveučilišni/a magistar/magistra ekologije i zaštite prirode (sveučilišni/a magistar/magistra ekologije i zaštite prirode)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-07-29 12:05:58