prikaz prve stranice dokumenta Dinamika GTPaza Rac1 i njihova uloga u regulaciji polarnosti stanica Dictyostelium discoideum
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doctoral thesis
Dinamika GTPaza Rac1 i njihova uloga u regulaciji polarnosti stanica Dictyostelium discoideum
Zagreb: University of Zagreb, Faculty of Science, 2015. urn:nbn:hr:217:042354
Marinović, Maja
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Marinović, M. (2015). Dinamika GTPaza Rac1 i njihova uloga u regulaciji polarnosti stanica Dictyostelium discoideum (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:042354

Marinović, Maja. "Dinamika GTPaza Rac1 i njihova uloga u regulaciji polarnosti stanica Dictyostelium discoideum." Doctoral thesis, University of Zagreb, Faculty of Science, 2015. https://urn.nsk.hr/urn:nbn:hr:217:042354

Marinović, Maja. "Dinamika GTPaza Rac1 i njihova uloga u regulaciji polarnosti stanica Dictyostelium discoideum." Doctoral thesis, University of Zagreb, Faculty of Science, 2015. https://urn.nsk.hr/urn:nbn:hr:217:042354

Marinović, M. (2015). 'Dinamika GTPaza Rac1 i njihova uloga u regulaciji polarnosti stanica Dictyostelium discoideum', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 14 July 2024, https://urn.nsk.hr/urn:nbn:hr:217:042354

Marinović M. Dinamika GTPaza Rac1 i njihova uloga u regulaciji polarnosti stanica Dictyostelium discoideum [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2015 [cited 2024 July 14] Available at: https://urn.nsk.hr/urn:nbn:hr:217:042354

M. Marinović, "Dinamika GTPaza Rac1 i njihova uloga u regulaciji polarnosti stanica Dictyostelium discoideum", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:217:042354

Metadata
TitleDinamika GTPaza Rac1 i njihova uloga u regulaciji polarnosti stanica Dictyostelium discoideum
Title (english)Dynamics of Rac1 GTPases and their role in the regulation of cell polarity in Dictyostelium discoideum
AuthorMaja Marinović
MentorIgor Weber (mentor)
Committee memberMaja Osmak (član povjerenstva)
Committee memberMaja Herak Bosnar (član povjerenstva)
Committee memberRenata Matoničkin Kepčija (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2015, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		576 - Cellular and subcellular biology. Cytology
Abstract
Proteini Rac su jedini predstavnici obitelji malih GTPaza Rho u organizmu Dictyostelium discoideum. Rezultati dosadašnjih istraživanja ukazuju na dvojaku ulogu Rac1 u regulaciji polimerizacije aktina, koja je ključna odrednica stanične polarnosti. Osim što na prednjem kraju stanice aktivacijom kompleksa Scar/WAVE potiče polimerizaciju aktina posredovanu kompleksom Arp2/3, Rac1 istodobno regulira stabilnost stražnjeg kraja kao inicijator stvaranja kompleksa jednog od IQGAP proteina, DGAP1 ili GAPA, s heterodimerom korteksilina. Kako bi dobili detaljniji uvid u funkcije tri izoforme proteina Rac1 iz D. discoideum, Rac1A, 1B i 1C, konstruirali smo poboljšanu probu koja je specifična za aktivni oblik GTPaze Rac1, a sastoji se od domene GBD iz proteina DPAKa fuzionirane sa žutim fluorescentnim proteinom. Praćenjem lokalizacije probe DPAKa_GBD-DYFP, kao i njene kolokalizacije s probama za druge proteine relevantne za organizaciju aktinskog citoskeleta, dobili smo uvid u vremensko-prostorne odnose glavnih komponenti ovog dinamičnog sustava u živim stanicama. Osim toga, analiza mutanta deficijentnog za dvije izoforme Rac1, 1B i 1C, pokazala je da, unatoč velikoj međusobnoj sličnosti, tri izoforme proteina Rac1 nemaju potpuno preklapajuće funkcije u stanicama D. discoideum.
Abstract (english)
Rac proteins are the only members of the Rho family of small GTPases present in Dictyostelium discoideum. It has been shown that Rac1 proteins play a dual role in the regulation of actin polymerization, which is the key determinant of cell polarity. At the front end of a cell Rac1A activates the Scar/WAVE complex and stimulates Arp2/3-mediated actin polymerization, while it simultaneously regulates stability of the rear end by initiating formation of a complex containing one of the IQGAP-related proteins, DGAP1 or GAPA, and the cortexillin heterodimer. To gain more insight into the roles of three highly homologous Rac1 proteins in D. discoideum, Rac1A, 1B and 1C, we developed an improved probe for active Rac1 GTPases. The probe consists of the GBD domain from DPAKa fused with the yellow fluorescent protein. We monitored localization of the DPAKa_GBD probe, and it's colocalization with probes for other proteins involved in organization of the actin cytoskeleton, to obtain information about spatio-temporal relations between main components of this dynamic system in live cells. Functional analysis of D. discoideum Rac1B/1C-deficient strain revealed that, although three Rac1 proteins are highly homologous, their roles are not completely redundant.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:042354
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
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Created on2017-05-17 09:24:44