prikaz prve stranice dokumenta Rekombinogenost palindroma i kvazipalindroma u genomu kvasca Saccharomyces cerevisiae
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doctoral thesis
Rekombinogenost palindroma i kvazipalindroma u genomu kvasca Saccharomyces cerevisiae
Zagreb: University of Zagreb, Faculty of Science, 2015. urn:nbn:hr:217:720503
Miklenić, Marina
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Miklenić, M. (2015). Rekombinogenost palindroma i kvazipalindroma u genomu kvasca Saccharomyces cerevisiae (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:720503

Miklenić, Marina. "Rekombinogenost palindroma i kvazipalindroma u genomu kvasca Saccharomyces cerevisiae." Doctoral thesis, University of Zagreb, Faculty of Science, 2015. https://urn.nsk.hr/urn:nbn:hr:217:720503

Miklenić, Marina. "Rekombinogenost palindroma i kvazipalindroma u genomu kvasca Saccharomyces cerevisiae." Doctoral thesis, University of Zagreb, Faculty of Science, 2015. https://urn.nsk.hr/urn:nbn:hr:217:720503

Miklenić, M. (2015). 'Rekombinogenost palindroma i kvazipalindroma u genomu kvasca Saccharomyces cerevisiae', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 14 January 2025, https://urn.nsk.hr/urn:nbn:hr:217:720503

Miklenić M. Rekombinogenost palindroma i kvazipalindroma u genomu kvasca Saccharomyces cerevisiae [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2015 [cited 2025 January 14] Available at: https://urn.nsk.hr/urn:nbn:hr:217:720503

M. Miklenić, "Rekombinogenost palindroma i kvazipalindroma u genomu kvasca Saccharomyces cerevisiae", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:217:720503

Metadata
TitleRekombinogenost palindroma i kvazipalindroma u genomu kvasca Saccharomyces cerevisiae
Title (english)Recombinogenicity of palindromes and quasipalindromes in the yeast Saccharomyces cerevisiae genome
AuthorMarina Miklenić
MentorIvan Krešimir Svetec (mentor)
MBZ: 210466
Committee memberVladimir Mrša (član povjerenstva)
Committee memberVišnja Besendorfer (član povjerenstva)
Committee memberKsenija Zahradka (član povjerenstva)
Committee memberRenata Teparić (član povjerenstva)
MBZ: 209535
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2015, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		575 - General genetics. General cytogenetics
Abstract
U ovom radu istražena je rekombinogenost savršenih palindroma i kvazipalindroma s kratkim razmaknicama u genomu kvasca Saccharomyces cerevisiae. Pokazano je da minimalna duljina razmaknice koja statistički značajno smanjuje rekombinogenost palindroma duljine 126 pb iznosi 6 pb, a razmaknica duljine 10 pb u potpunosti anulira rekombinogenost palindroma duljine do 150 pb. Nadalje, pokazano je da na rekombinogenost palindroma in vivo može utjecati temperatura okoline. Osim toga, rekombinogenost palindroma i kvazipalindroma istražena je u nizu mutanata kvasca, te je utvrđeno da helikaza Sgs1 smanjuje rekombinogenost kvazipalindroma i savršenih palindroma, dok endonukleaza Rad27 smanjuje rekombinogenost i učestalost gubitka savršenih palindroma. Nadalje, pokazno je da protein Ku70 povećava rekombinogenost palindroma in vivo, najvjerojatnije stabilizacijom strukture kruciforma. Također, konstruiran je eksperimentalni sustav u kojem rekombinogenost savršenih palindroma korelira s intenzitetom boje kolonija kvasca, a može se koristiti i za identifikaciju novih gena/proteina uključenih u rekombinogenost palindroma na temelju komplementacije mutacije u mutantima dobivenim nasumičnom mutagenezom.
Abstract (english)
In this thesis recombinogenicity of perfect palindromes and quasipalindromes in the yeast Saccharomyces cerevisiae genome was investigated. It was shown that a minimal spacer length which influences recombinogenicity of a 126 bp palindrome is 6 bp, while 10 bp long spacer completely abolishes recombinogenicity of palindromes up to 150 bp long. Furthermore, it was shown that growth temperature can affect palindrome recombinogenicity in vivo. Palindrome recombinogenicity was investigated in a series of yeast mutants and it was shown that helicase Sgs1 reduces recombinogenicity of both perfect palindromes and quasipalindromes, while endonuclease Rad27 reduces recombinogenicity and deletion frequency of perfect palindromes. On the other hand, protein Ku70 increases palindrome recombinogenicity, possibly by stabilisation of cruciform structures. Furthermore, an experimental system based on correlation between yeast colony colour and palindrome recombinogenicity was developed and it was shown that this system can be used to identify novel proteins responsible for palindrome recombinogenicity using complementation of mutants obtained by random mutagenesis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:720503
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-17 09:46:04