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master's thesis
The role of DAF-41 in lifespan of Caenorhabditis elegans
Zagreb: University of Zagreb, Faculty of Science, 2024. urn:nbn:hr:217:564872
Sačer, Margareta
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Sačer, M. (2024). The role of DAF-41 in lifespan of Caenorhabditis elegans (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:564872

Sačer, Margareta. "The role of DAF-41 in lifespan of Caenorhabditis elegans." Master's thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:564872

Sačer, Margareta. "The role of DAF-41 in lifespan of Caenorhabditis elegans." Master's thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:564872

Sačer, M. (2024). 'The role of DAF-41 in lifespan of Caenorhabditis elegans', Master's thesis, University of Zagreb, Faculty of Science, accessed 09 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:564872

Sačer M. The role of DAF-41 in lifespan of Caenorhabditis elegans [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2024 [cited 2024 November 09] Available at: https://urn.nsk.hr/urn:nbn:hr:217:564872

M. Sačer, "The role of DAF-41 in lifespan of Caenorhabditis elegans", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:217:564872

Metadata
TitleThe role of DAF-41 in lifespan of Caenorhabditis elegans
Title (croatian)Uloga DAF-41 u životnom vijeku Caenorhabditis elegans
AuthorMargareta Sačer
MentorAdam Antebi (mentor)
MentorDubravka Hranilović (komentor)
Committee memberDubravka Hranilović (predsjednik povjerenstva)
Committee memberBiljana Balen (član povjerenstva)
Committee memberDunja Leljak-Levanić (član povjerenstva)
Committee memberInga Urlić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2024-07-15, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Tiku et al. (2017) published that loss of the protein NCL-1 (abnormal NuCLeoli 1) abolishes the extended lifespan in major Caenorhabditis elegans longevity pathways, including the germline-deficient glp-1 (abnormal Germ Line Proliferation 1) C. elegans mutant. Recent unpublished work from the Antebi group identified a protein DAF-41 (Abnormal Dauer Formation 41), an HSP-90 (heat shock protein 90) co-chaperone, as a potential downstream regulator of glp-1ncl-1 lifespan. I investigated the role of daf-41 in glp-1ncl-1 lifespan, finding that daf-41 downregulation extended the lifespan of glp-1ncl-1 worms. Moreover, I performed RT-qPCR analysis of genes associated with the insulin signaling pathway (daf-16, lipl-4, sod3, dod-3), heat-shock response (hsf-1, hsp-70, hsp-16.2, hsp-4), and steroid pathway (daf-12, fard-1, cdr-6, daf-36), quantified GFP-tagged HSF-1 and DAF-16 transcription factor activity and measured thermotolerance and oxidative stress resistance in different mutant backgrounds. Changes in gene expression, DAF-16 and HSF-1 activity, thermotolerance, and oxidative stress resistance did not clearly explain the mechanism behind this lifespan extension.
Abstract (english)
Tiku i suradnici (2017) primijetili su da delecija gena tumor-supresora NCL-1 (eng. Nucleoli 1) skraćuje produljeni životni vijek u više dugovječnih modela Caenorhabditis elegans, među kojima i u dvostrukom mutantu glp-1ncl-1 (eng. Abnormal Germ Line Proliferation 1). Prethodno neobjavljena istraživanja Antebija i suradnika nedavno su identificirala protein DAF-41 (eng. Abnormal Dauer Formation 41), ko-šaperon proteina HSP-90 (eng. Heat Shock Protein 90) kao potencijalnog nizvodnog regulatora životnog vijeka mutanta glp-1ncl-1. Otkrila sam da utišavanje gena daf-41 produžuje životni vijek C. elegans mutanta glp-1ncl-1. Kako bih otkrila potencijalni signalni put ovog fenomena, provela sam RT-qPCR analizu gena inzulinskog signalnog puta (daf-16, lipl-4, sod-3, dod-3), odgovora na toplinski šok (hsf-1, hsp70, hsp-16.2, hsp-4) i steroidnog signalnog puta (daf-12, fard-1, cdr-6, daf-36). Zatim sam kvantificirala aktivnost fluorescentno obilježenih transkripcijskih faktora HSF-1 i DAF-16 te mjerila otpornost oblića na toplinski i oksidativni stres. Međutim, promjene u ekspresiji gena, aktivnosti DAF-16 i HSF-1, otpornost na toplinski i oksidativni stres nisu dali jasan uvid u mehanizam produženja životnog vijeka mutanata glp-1ncl-1 s utišanim genom daf-41.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:217:564872
Study programmeTitle: Molecular Biology-University graduate programme
Study programme type: university
Study level: graduate
Academic / professional title: sveučilišni/a magistar/magistra molekularne biologije (sveučilišni/a magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-08-26 13:24:14