prikaz prve stranice dokumenta Uloga proupalnih i protuupalnih mehanizama u razvoju rezistencije posredovane ABC transporterima u upalnim bolestima crijeva
  Download
PDF 2.07 MB
doctoral thesis
Uloga proupalnih i protuupalnih mehanizama u razvoju rezistencije posredovane ABC transporterima u upalnim bolestima crijeva
Zagreb: University of Zagreb, Faculty of Science, 2015. urn:nbn:hr:217:827987
Savić Mlakar, Ana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Savić Mlakar, A. (2015). Uloga proupalnih i protuupalnih mehanizama u razvoju rezistencije posredovane ABC transporterima u upalnim bolestima crijeva (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:827987

Savić Mlakar, Ana. "Uloga proupalnih i protuupalnih mehanizama u razvoju rezistencije posredovane ABC transporterima u upalnim bolestima crijeva." Doctoral thesis, University of Zagreb, Faculty of Science, 2015. https://urn.nsk.hr/urn:nbn:hr:217:827987

Savić Mlakar, Ana. "Uloga proupalnih i protuupalnih mehanizama u razvoju rezistencije posredovane ABC transporterima u upalnim bolestima crijeva." Doctoral thesis, University of Zagreb, Faculty of Science, 2015. https://urn.nsk.hr/urn:nbn:hr:217:827987

Savić Mlakar, A. (2015). 'Uloga proupalnih i protuupalnih mehanizama u razvoju rezistencije posredovane ABC transporterima u upalnim bolestima crijeva', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:827987

Savić Mlakar A. Uloga proupalnih i protuupalnih mehanizama u razvoju rezistencije posredovane ABC transporterima u upalnim bolestima crijeva [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2015 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:827987

A. Savić Mlakar, "Uloga proupalnih i protuupalnih mehanizama u razvoju rezistencije posredovane ABC transporterima u upalnim bolestima crijeva", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:217:827987

Metadata
TitleUloga proupalnih i protuupalnih mehanizama u razvoju rezistencije posredovane ABC transporterima u upalnim bolestima crijeva
Title (english)The role of proinflammatory and anti-inflammatory mechanisms in development of resistance mediated by ABC transporters in inflammatory bowel diseases
AuthorAna Savić Mlakar
MentorIvna Svoboda-Beusan (mentor)
MBZ: 45713
MentorKrešo Bendelja (sumentor)
MBZ: 229091
Committee memberNada Oršolić (član povjerenstva)
Committee memberBranko Troskot (član povjerenstva)
MBZ: 117465
Committee memberMaja Matulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2015, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
ABC transporteri sudjeluju u očuvanju crijevne barijere, te utječu na biodostupnost lijekova. Promjene njihove ekspresije mogu doprinijeti narušavanju barijere i razvoju upale, kao što je slučaj u upalnim bolestima crijeva. Njihova uloga u patogenezi upalnih bolesti crijeva nije u potpunosti razjašnjena. U ovom istraživanju odredili smo ekspresiju triju transportera: MDR1, MRP1 i BCRP, kod odraslih i djece oboljele od Crohnove bolesti (odrasli; n=25, djeca; n=26) i ulceroznog kolitisa (odrasli; n=27, djeca; n=20). Paralelno smo odredili i ekspresiju proupalnih citokina interferona (IFN)-γ, interleukina (IL)-2, IL-6, IL-1β i IL-17A, kao i njihove negativne regulatore, SOCS1 i SOCS3. Ispitanici su podijeljeni u četiri grupe: neliječeni bolesnici dijagnosticirani de novo, grupa bolesnika pod terapijom, te grupa u remisiji odnosno grupa zdrave kontrole (odrasli; n=10, djeca; n=15). Ovo istraživanje pokazuje izmijenjene ekspresije transportera koji koreliraju s povišenim ekspresijama citokina u oboljelih ispitanika. Nadalje, povišena ekspresija proupalnih citokina, popraćena povišenim ekspresijama njihovih negativnih regulatora, potvrđuju upalni proces kao dominantni patološki mehanizam u upalnim bolestima crijeva. Dakle, promjene ekspresija ABC transportera mogle bi imati funkcionalnu važnost u progresiji bolesti i liječenju.
Abstract (english)
Efflux transporters are crucial for the maintenance of gut homeostasis and influence drug bioavailability. Changes in their expression can disrupt the barrier which consequently results with inflammatory response, as observed in inflammatory bowel diseases (IBD). Their exact role in IBD pathogenesis is inconclusive. We have measured the expression of transporeters MDR1, MRP1 and BCRP in pediatric and adult patients with Crohn's disease (adults; n=25, pediatric; n=26) and ulcerative colitis (adults; n=27, pediatric; n=20). In paralell, we have assessed the expression of inflammatory cytokines IFN-γ , IL-2, IL-6, IL-1β and IL-17A, as well as their negative regulators, SOCS1 and SOCS3. Subjects were grouped as: newly diagnosed/untreated, active disease/treated, remission and controls (adults; n=10, pediatric; n=15). We showed that altered expression of efflux transporters correlated with cytokine expressions in IBD patients compared to controls. Furthermore, high cytokine expression, followed by high expression of their matched negative regulators, confirmed inflammatory process as the dominant pathological mechanism in IBD. In conclusion, alterations in ABC transporter expression could have functional importance in disease progression and treatment.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:827987
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-05-17 10:43:06