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doctoral thesis
UTJECAJ NUKLEOZIDNIH ANALOGA NA GENSKU VARIJABILNOST I INFEKTIVNOST VIRUSA ZAUŠNJAKA
Zagreb: University of Zagreb, Faculty of Science, 2024. urn:nbn:hr:217:181057
Jurković, Mirna
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Jurković, M. (2024). UTJECAJ NUKLEOZIDNIH ANALOGA NA GENSKU VARIJABILNOST I INFEKTIVNOST VIRUSA ZAUŠNJAKA (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:181057

Jurković, Mirna. "UTJECAJ NUKLEOZIDNIH ANALOGA NA GENSKU VARIJABILNOST I INFEKTIVNOST VIRUSA ZAUŠNJAKA." Doctoral thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:181057

Jurković, Mirna. "UTJECAJ NUKLEOZIDNIH ANALOGA NA GENSKU VARIJABILNOST I INFEKTIVNOST VIRUSA ZAUŠNJAKA." Doctoral thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:181057

Jurković, M. (2024). 'UTJECAJ NUKLEOZIDNIH ANALOGA NA GENSKU VARIJABILNOST I INFEKTIVNOST VIRUSA ZAUŠNJAKA', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 11 September 2024, https://urn.nsk.hr/urn:nbn:hr:217:181057

Jurković M. UTJECAJ NUKLEOZIDNIH ANALOGA NA GENSKU VARIJABILNOST I INFEKTIVNOST VIRUSA ZAUŠNJAKA [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2024 [cited 2024 September 11] Available at: https://urn.nsk.hr/urn:nbn:hr:217:181057

M. Jurković, "UTJECAJ NUKLEOZIDNIH ANALOGA NA GENSKU VARIJABILNOST I INFEKTIVNOST VIRUSA ZAUŠNJAKA", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:217:181057

Metadata
TitleUTJECAJ NUKLEOZIDNIH ANALOGA NA GENSKU VARIJABILNOST I INFEKTIVNOST VIRUSA ZAUŠNJAKA
Title (english)INFLUENCE OF NUCLEOSIDE ANALOGUES ON THE HETEROGENEITY AND INFECTIVITY OF MUMPS VIRUS
AuthorMirna Jurković
MentorMaja Jagušić (mentor)
Committee memberSnježana Židovec-Lepej (predsjednik povjerenstva)
Committee memberDubravko Forčić (član povjerenstva)
Committee memberSilvija Černi (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2024, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
Pojava epidemija uzrokovanih virusom zaušnjaka čak i u procijepljenim populacijama ukazuje na potrebu razvoja učinkovitijih cjepiva i specifične antivirusne terapije. U ovom istraživanju ispitan je potencijal triju nukleozidnih analoga (ribavirina, 5-fluorouracila, 5-azacitidina) za moduliranje genske varijabilnosti ovog virusa u smjeru njegove atenuacije ili ekstinkcije metodama virusne kvantifikacije te analize genoma na temelju rezultata sekvenciranja nove generacije. Pokazano je da sva tri nukleozidna analoga u genom uvode specifične promjene, mijenjaju stopu mutacije i smanjuju titar virusa u staničnoj kulturi. Virusi tretirani 5-azacitidinom razvili su rezistentnost na nukleozidni analog. Tretman virusa koncentracijom ribavirina od 500 μM doveo je do ekstinkcije virusa. Rezultati ovog istraživanja pridonose boljem razumijevanju biologije i evolucije virusa zaušnjaka, naglašavajući potencijal mutageneze kao jednog od mehanizama djelovanja sva tri nukleozidna analoga na ovaj virus.
Abstract (english)
Intermittent mumps virus outbreaks within vaccinated populations underscore the imperative for advancing more efficacious vaccines and specific antiviral therapy. This study investigates the potential of three nucleoside analogs (ribavirin, 5-fluorouracil, 5-azacytidine) for modulating the genetic variability of this virus towards its attenuation or extinction using virus quantification methods and next-generation sequencing genome analysis. Results show that these analogs induce specific genomic changes, alter mutation rates, and reduce virus titre in cell culture. Notably, viruses treated with 5-azacytidine exhibit resistance to the nucleoside analog. Additionally, a 500 μM ribavirin concentration leads to virus extinction. The research enhances understanding of mumps virus biology and evolution, emphasizing mutagenesis as a key mechanism for all three nucleoside analogs.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:181057
Promotion2024
ProjectNumber: KK.01.1.1.01.0006
Title: Jačanje kapaciteta CERVIRVAC-a za istraživanja u virusnoj imunologiji i vakcinologiji
Title: Strengthening the capacity of CerVirVac (Croatian Centre of Excellence for Virus Immunology and Vaccines) for research in viral immunology and vaccinology
Leader: Stipan Jonjić
Jurisdiction: eu
Funder: Europska komisija
Funding stream: European Regional Development Fund
Study programmeTitle: Doctoral study
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-09-03 10:35:51