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doctoral thesis
BIOINFORMATIČKA ANALIZA UČINKA STANIČNE PARABIOZE I GENSKE EKSPRESIJE NA NASTANAK I METASTAZIRANJE TUMORA
Zagreb: University of Zagreb, Faculty of Science, 2024. urn:nbn:hr:217:440716
Vidović, Tinka
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Vidović, T. (2024). BIOINFORMATIČKA ANALIZA UČINKA STANIČNE PARABIOZE I GENSKE EKSPRESIJE NA NASTANAK I METASTAZIRANJE TUMORA (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:440716

Vidović, Tinka. "BIOINFORMATIČKA ANALIZA UČINKA STANIČNE PARABIOZE I GENSKE EKSPRESIJE NA NASTANAK I METASTAZIRANJE TUMORA." Doctoral thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:440716

Vidović, Tinka. "BIOINFORMATIČKA ANALIZA UČINKA STANIČNE PARABIOZE I GENSKE EKSPRESIJE NA NASTANAK I METASTAZIRANJE TUMORA." Doctoral thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:440716

Vidović, T. (2024). 'BIOINFORMATIČKA ANALIZA UČINKA STANIČNE PARABIOZE I GENSKE EKSPRESIJE NA NASTANAK I METASTAZIRANJE TUMORA', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:440716

Vidović T. BIOINFORMATIČKA ANALIZA UČINKA STANIČNE PARABIOZE I GENSKE EKSPRESIJE NA NASTANAK I METASTAZIRANJE TUMORA [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2024 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:440716

T. Vidović, "BIOINFORMATIČKA ANALIZA UČINKA STANIČNE PARABIOZE I GENSKE EKSPRESIJE NA NASTANAK I METASTAZIRANJE TUMORA", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:217:440716

Metadata
TitleBIOINFORMATIČKA ANALIZA UČINKA STANIČNE PARABIOZE I GENSKE EKSPRESIJE NA NASTANAK I METASTAZIRANJE TUMORA
Title (english)BIOINFORMATIC ANALYSIS OF INFLUENCE OF CELLULAR PARABIOSIS AND GENE EXPRESSION ON TUMORIGENESIS AND METASTASIS FORMATION
AuthorTinka Vidović
MentorMiroslav Radman (mentor)
Committee memberRosa Karlić (predsjednik povjerenstva)
Committee memberNada Oršolić (član povjerenstva)
Committee memberPetar Ozretić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2024-09-02, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
U ovom radu se istražio utjecaj stanične parabioze i genske ekspresije na specifičnost nastanka tumora. Pokazali smo da tkiva koja povećano izražavaju gene koje pripadaju kategorijama nedostatak IRAK4 te MET aktivira Rap1 i Rac1 su podložnija nastanku metastazi. Navedeni rezultat je u skladu s početnom hipotezom da se manipulacijom staničnom parabiozom mogu spriječiti maligni tumori budući da su Rho-GTPaze regulatori aktinskog citoskeleta i stanične migracije. Koristeći metodu ponderirane genske koekspresije pronađeni su ključni geni za nastanak tumora, koji su se usporedili s normalnom ekspresijom gena u svim tkivima. Cilj ove analize je bio vidjeti kako su ključni geni u tumorima izraženi u svim normalnim tkivima, budući da obrazac ekspresije gena u normalnom tkivu može biti uzrok specifičnosti metastaziranja. Također su pronađeni i diferencijalno eksprimirani (DE) geni između ranih i kasnih stadija, te se na temelju ekspresije DE gena postignuti odlični rezultati razlikovanja ranih i kasnih stadija tumora metodom potpornih vektora.
Abstract (english)
In this work, the influence of cellular parabiosis and gene expression on the specificity of tumor formation was investigated. We showed that tissues that overexpressed genes belonging to the IRAK4 deficiency and MET activates Rap1 and Rac1 categories are more susceptible to metastases. This result is in accordance with the initial hypothesis that malignant tumors can be prevented by manipulation of cellular parabiosis since Rho-GTPases are regulators of the actin cytoskeleton and cell migration. Using the method of weighted gene co-expression, core genes for the formation of tumors were found, which were compared with normal gene expression in all tissues. The aim of this analysis was to see how core genes in tumors are expressed in all normal tissues, since the pattern of gene expression in normal tissue may be the cause of the specificity of metastasis. Differentially expressed (DE) genes between early and late stages were also found, and based on the expression of the DE gene, excellent results were achieved in distinguishing between early and late tumor stages by support vector machine method.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:440716
Promotion2024
Study programmeTitle: Doctoral study
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-09-12 16:29:46