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doctoral thesis
Uloga izoformi ciklooksigenaza u tumorigenezi sporadičnoga karcinoma debeloga crijeva
Zagreb: University of Zagreb, Faculty of Science, 2014. urn:nbn:hr:217:610301
Catela Ivković, Tina
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Catela Ivković, T. (2014). Uloga izoformi ciklooksigenaza u tumorigenezi sporadičnoga karcinoma debeloga crijeva (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:610301

Catela Ivković, Tina. "Uloga izoformi ciklooksigenaza u tumorigenezi sporadičnoga karcinoma debeloga crijeva." Doctoral thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:610301

Catela Ivković, Tina. "Uloga izoformi ciklooksigenaza u tumorigenezi sporadičnoga karcinoma debeloga crijeva." Doctoral thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:610301

Catela Ivković, T. (2014). 'Uloga izoformi ciklooksigenaza u tumorigenezi sporadičnoga karcinoma debeloga crijeva', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:610301

Catela Ivković T. Uloga izoformi ciklooksigenaza u tumorigenezi sporadičnoga karcinoma debeloga crijeva [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2014 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:610301

T. Catela Ivković, "Uloga izoformi ciklooksigenaza u tumorigenezi sporadičnoga karcinoma debeloga crijeva", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:217:610301

Metadata
TitleUloga izoformi ciklooksigenaza u tumorigenezi sporadičnoga karcinoma debeloga crijeva
Title (english)The role of cyclooxygenase isoforms in sporadic colorectal carcinogenesis
AuthorTina Catela Ivković
MentorSanja Kapitanović (mentor)
Committee memberNada Oršolić (član povjerenstva)
Committee memberMarijana Popović Hadžija (član povjerenstva)
Committee memberMaja Sirotković-Skerlev (član povjerenstva)
MBZ: 119524
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2014, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
Ciklooksigenaze su ključni enzimi u biosintezi prostanoida. Postoje dvije izoforme: COX-1 koja je povezana s održavanjem fizioloških uvjeta u sluznici debelog crijeva; te inducibilna izoforma COX-2 koja je uglavnom povezana s patološkim procesima uključujući karcinom debelog crijeva. Cilj ovog istraživanja bio je ispitati ulogu COX izoformi u razvoju i napredovanju sporadičnoga karcinoma debeloga crijeva, mehanizme njihove regulacije te povezanost regulatornih polimorfizama gena COX-1 i COX-2 sa sklonošću obolijevanju i ekspresijom mRNA i proteina. Naši rezultati su pokazali da COX-1 ima zaštitnu, a COX-2 tumorigenu ulogu u sluznici debelog crijeva. Gubitak COX-1 učestali je događaj u karcinogenezi, dok COX-2 pokazuje veću varijaciju između uzoraka. Porast COX-2 ekspresije povezan je s lošijim preživljenjem oboljelih. Dokazali smo povezanost regulatornih polimorfizama sa sklonošću obolijevanju i ekspresijom gena COX-2. Ekspresija proteina odgovara mRNA ekspresiji te nema povezanosti s ekspresijom miR-200 što pokazuje da je ekspresija gena COX-1 i COX-2 primarno regulirana na razini transkripcije.
Abstract (english)
Cyclooxygenases are the key enzymes in prostanoid biosynthesis. Two isoforms exist: COX-1, associated with maintainance of physiological conditions in colon; and inducible COX-2 that is mainly related to various pathologies including colorectal cancer. The aim of our study was to examine the role of COX isoforms in development and progression of sporadic colorectal cancer, mechanisms of their regulation and whether regulatory polymorphisms in COX genes are related to cancer susceptibility, mRNA and protein expression. Our results show that COX-1 has protective, and COX-2 tumorigenic role in colorectal mucosa. Loss of COX-1 is very frequent in carcinogenesis, while COX-2 shows greater variation between samples. Higher COX-2 expression correlates to worse patient survival. We proved the role of regulatory polymorphisms in cancer susceptibility, and COX-2 expression. Protein expression correlates with mRNA expression, and there is no associtation with miR-200, which indicates transcriptional regulation as the key regulatory mechanism of COX expression.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:610301
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-05-17 12:39:30