prikaz prve stranice dokumenta Utjecaj potaknute hematopoeze na diferencijaciju i aktivnost mišjih osteoklasta
No public access
doctoral thesis
Utjecaj potaknute hematopoeze na diferencijaciju i aktivnost mišjih osteoklasta
Zagreb: University of Zagreb, Faculty of Science, 2014. urn:nbn:hr:217:730668
Kuzmac, Sania
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Kuzmac, S. (2014). Utjecaj potaknute hematopoeze na diferencijaciju i aktivnost mišjih osteoklasta (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:730668

Kuzmac, Sania. "Utjecaj potaknute hematopoeze na diferencijaciju i aktivnost mišjih osteoklasta." Doctoral thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:730668

Kuzmac, Sania. "Utjecaj potaknute hematopoeze na diferencijaciju i aktivnost mišjih osteoklasta." Doctoral thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:730668

Kuzmac, S. (2014). 'Utjecaj potaknute hematopoeze na diferencijaciju i aktivnost mišjih osteoklasta', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:730668

Kuzmac S. Utjecaj potaknute hematopoeze na diferencijaciju i aktivnost mišjih osteoklasta [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2014 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:730668

S. Kuzmac, "Utjecaj potaknute hematopoeze na diferencijaciju i aktivnost mišjih osteoklasta", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:217:730668

Metadata
TitleUtjecaj potaknute hematopoeze na diferencijaciju i aktivnost mišjih osteoklasta
Title (english)Effect of induced hematopoiesis on osteoclast differentiation and activity in mice
AuthorSania Kuzmac
MentorVedran Katavić (mentor)
MBZ: 219360
Committee memberTatjana Bordukalo Nikšić (član povjerenstva)
MBZ: 231170
Committee memberZoran Tadić (član povjerenstva)
Committee memberDanka Grčević (član povjerenstva)
MBZ: 210400
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2014, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		57 - Biological sciences. Physical anthropology. Biodiversity
Abstract
Osteoklasti su komponenta hematopoetske niše koštane srži, no njihova uloga u regulaciji hematopoeze nije dovoljno istražena i dosadašnji rezultati su proturječni. Istražili smo utjecaj potaknute hematopoeze nakon akutnog i kroničnog gubitka krvi na diferencijaciju i aktivnost mišjih osteoklasta. U modelu akutnog gubitka krvi naglasak smo stavili na preteče osteoklasta i izražaj gena uključenih u osteoklastogenezu te potencijal stanica koštane srži da se u in vitro uvjetima diferenciraju u koštane stanice. U modelu kroničnog gubitka krvi smo istražili učinak na aktivnost koštanih stanica odnosno koštanu pregradnju. Modelom akutnog gubitka krvi smo pokazali da potaknuta hematopoeza ne utječe na broj preteča osteoklasta, ali da potiče njihovo sazrijevanje. Model kroničnog gubitka krvi je pokazao da dugoročan gubitak krvi nema značajnog utjecaja na aktivnost osteoklasta i pregradnju trabekularne kosti. Rezultati su pokazali da krvarenje ne potiče aktivnost osteoklasta i koštanu razgradnju kako bi stvorili dodatan prostor za koštanu srž.
Abstract (english)
Osteoclasts are components of hematopoietic bone marrow niche, but their role as hematopoietic contributors are still controversial. We aimed to investigate whether an acute and chronic blood loss and the consequent intense hematopoiesis would affect osteoclast differentiation and activity in mice. In the model of acute blood loss research was focused on specific subpopulations of osteoclast precursors, expression of osteoclastogenesis- and osteoblastogenesis-related genes and the potential of bone marrow cells to form osteoclast-like cells in vitro, while in the model of chronic blood loss on bone cells activity in vivo. There were no significant changes of cells with high osteoclastogenic potential, but we observed promoted osteoclast maturation following acute blood loss. Chronic blood loss had no effect on osteoclast activity and remodeling of trabecular bone. Our data demonstrate that blood loss does not promote osteoclast activity and osteoresorption to provide additional space for bone marrow.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:730668
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsClosed access
Terms of useLicence In copyright icon
Created on2017-05-18 10:08:55