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master's thesis
Utjecaj sirtuina 3 na oksidativno i metaboličko stanje tkiva bubrega i mozga mužjaka i ženke miša
Zagreb: University of Zagreb, Faculty of Science, 2024. urn:nbn:hr:217:931606
Kaloper, Marta
University of Zagreb
Faculty of Science

Institutional repository: Repository of the Faculty of Science

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Kaloper, M. (2024). Utjecaj sirtuina 3 na oksidativno i metaboličko stanje tkiva bubrega i mozga mužjaka i ženke miša (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:931606

Kaloper, Marta. "Utjecaj sirtuina 3 na oksidativno i metaboličko stanje tkiva bubrega i mozga mužjaka i ženke miša." Master's thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:931606

Kaloper, Marta. "Utjecaj sirtuina 3 na oksidativno i metaboličko stanje tkiva bubrega i mozga mužjaka i ženke miša." Master's thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:931606

Kaloper, M. (2024). 'Utjecaj sirtuina 3 na oksidativno i metaboličko stanje tkiva bubrega i mozga mužjaka i ženke miša', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:931606

Kaloper M. Utjecaj sirtuina 3 na oksidativno i metaboličko stanje tkiva bubrega i mozga mužjaka i ženke miša [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2024 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:931606

M. Kaloper, "Utjecaj sirtuina 3 na oksidativno i metaboličko stanje tkiva bubrega i mozga mužjaka i ženke miša", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:217:931606

Metadata
TitleUtjecaj sirtuina 3 na oksidativno i metaboličko stanje tkiva bubrega i mozga mužjaka i ženke miša
Title (english)Impact of sirtuin 3 on the oxidative and metabolic state of kidney and brain tissue in male and female mouse
AuthorMarta Kaloper
MentorRobert Belužić (mentor)
MentorDubravka Hranilović (komentor)
Committee memberDubravka Hranilović (predsjednik povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
Committee memberBiljana Balen (član povjerenstva)
Committee memberPetra Korać (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
Zagreb
Defense date and country2024-09-16, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Abstract
Sirtuin 3 (SIRT3) pripada obitelji proteina sirtuina i glavna je mitohondrijska NAD+ ovisna deacetilaza. Sudjeluje u regulaciji staničnog metabolizma, oksidativnog stresa i transkripcije. Njegova pojačana aktivacija, što suplementima, a što zbog polimorfizama prirodno prisutnih u populaciji, pokazala se efikasnom metodom produljenja životnog vijeka modelnih organizama i čovjeka. U ovom istraživanju analiziran je njegov utjecaj na oksidativno i metaboličko stanje u tkivu bubrega i mozga. Istraživanje je provedeno na osamnaest tjedana starim mužjacima i ženkama miševa divljeg tipa i miševa s nedostatkom proteina SIRT3, pri normalnoj prehrani. Analizirana je ekspresija proteina uključenih u antioksidativnu obranu i metabolizam metodom Western blot. Na uzorcima tkiva bubrega i mozga provedena je analiza oksidativnih oštećenja proteina analizom karbonilacije, odnosno oštećenja lipida lipidnom peroksidacijom. Dodatno je određena aktivnost antioksidativnih enzima katalaze i superoksid dismutaze. U analizi ekspresije uočen je utjecaj SIRT3 na količinu proteina SOD2 u bubregu i PPARγ u mozgu. Oksidativna oštećenja sugeriraju protektivno djelovanje na lipide u mozgu i proteine u bubregu, a aktivnost antioksidativnih enzima varira s obzirom na spol i tip organa.
Abstract (english)
Sirtuin 3 (SIRT3) belongs to the sirtuin protein family and is a key mitochondrial NAD+-dependent deacetylase. It is involved in regulating cellular metabolism, oxidative stress, and transcription. Its increased activation, either through supplements or due to naturally occurring polymorphisms in the population, has proven to be an effective method for extending the lifespan of model organisms and humans. In this study, its impact on oxidative and metabolic states in kidney and brain tissues was analyzed. The research was conducted on 18-week-old male and female wild-type mice and mice lacking the SIRT3 protein, under normal dietery conditions. The expression of proteins involved in antioxidant defense and metabolism was analyzed using the Western blot method. Oxidative damage to proteins in kidney and brain tissue samples was determined by analyzing carbonylation, and lipid damage was assessed through lipid peroxidation. Additionally, the activity of antioxidant enzymes catalase and superoxide dismutase was measured. The analysis of protein expression showed an influence of SIRT3 on the amount of SOD2 protein in the kidney and PPARγ in the brain. Oxidative damage suggests a protective effect on lipids in the brain and proteins in the kidney, while the activity of antioxidant enzymes varies depending on the sex and type of organ.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:931606
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-09-22 10:24:04