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doctoral thesis
Biološki učinci D-izomera metencefalina u eksperimentalnom modelu kemijskog oštećenja jetre miša paracetamolom
Zagreb: University of Zagreb, Faculty of Science, 2014. urn:nbn:hr:217:257223
Martinić, Roko
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Martinić, R. (2014). Biološki učinci D-izomera metencefalina u eksperimentalnom modelu kemijskog oštećenja jetre miša paracetamolom (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:257223

Martinić, Roko. "Biološki učinci D-izomera metencefalina u eksperimentalnom modelu kemijskog oštećenja jetre miša paracetamolom." Doctoral thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:257223

Martinić, Roko. "Biološki učinci D-izomera metencefalina u eksperimentalnom modelu kemijskog oštećenja jetre miša paracetamolom." Doctoral thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:257223

Martinić, R. (2014). 'Biološki učinci D-izomera metencefalina u eksperimentalnom modelu kemijskog oštećenja jetre miša paracetamolom', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 28 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:257223

Martinić R. Biološki učinci D-izomera metencefalina u eksperimentalnom modelu kemijskog oštećenja jetre miša paracetamolom [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2014 [cited 2024 December 28] Available at: https://urn.nsk.hr/urn:nbn:hr:217:257223

R. Martinić, "Biološki učinci D-izomera metencefalina u eksperimentalnom modelu kemijskog oštećenja jetre miša paracetamolom", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:217:257223

Metadata
TitleBiološki učinci D-izomera metencefalina u eksperimentalnom modelu kemijskog oštećenja jetre miša paracetamolom
Title (english)Biological effects of D- met-enkephalin isomer in a murine model of chemical liver injury induced by paracetamol
AuthorRoko Martinić
MentorNikola Štambuk (mentor)
MBZ: 160463
Committee memberMirko Hadžija (član povjerenstva)
MBZ: 15065
Committee memberGordana Lacković-Venturin (član povjerenstva)
Committee memberRanko Stojković (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2014, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
Peptidi i proteini u sisavaca i viših organizama građeni su od L-amino kiselina. Istraživanja peptida-enantiomera i upotreba D-amino kiselina je važan postupak za pripravu novih proteinskih struktura s različitim svojstvima u odnosu na nativne L-oblike. L-met-encefalin enantiomer posjeduje hepatoprotektivne odlike u mišjem modelu hepatotoksičnosti inducirane paracetamolom. Trenutno nema dostupnih podataka o osobinama i biološkim učincima Dmet- encefalin enantiomera, te je svrha ovog rada istražiti njegov hepatoprotektivni potencijal. D-enantiomer pokazao je zaštitni učinak u rasponu doza od 0,5 mg/kg do 20 mg/kg. Plazmatske aktivnosti enzima alanin aminotransferaza i aspartat aminotransferaza te nekroza jetre bile su značajno smanjene u usporedbi s kontrolnim životinjama koje su tretirane fiziološkom otopinom (p<0,01). Najjači potektivni učinak je uočen u životinja tretiranih s dozom od 5 mg/kg. Učinci su također ispitani u uvjetima blokade receptora i peptida. Pokazano je da su vjerojatno posredovani putem ζ i δ opioidnih receptora, a blokira ih naltrekson i antisens peptid IPPKY. Zaključeno je da su protektivni učinci D-met-encefalina usporedivi s učincima L-met-encefalina.
Abstract (english)
Peptides and proteins in mammals and higher organisms are composed of L-amino acids. The investigation of peptide-enantiomerism and the use of D-amino acids is important for the design of novel protein structures, with properties different from the native all L-forms. Lmet- enkephalin enantiomer exhibits strong protection in the mice model of acetaminophen induced hepatotoxicity. Howerver, there is currently no data available on the properties and biological effects of D-met-enkephalin enantiomer, and consequently we evaluated its hepatoprotective potential. D-enantiomer was shown to be protective in the dose range from 0.5 mg/kg to 20 mg/kg. Plasma AST, ALT and liver necrosis were significantly reduced in comparison to control animals treated with physiological saline (p>0.01). The best protection was observed in animals treated with a dose of 5 mg/kg. The effects of D-met-enkephalin were investigated from the standpoint of the receptor and peptide blockade. It was shown that they were probably mediated via δ and ζ opioid receptors, and blocked by naltrexone and antisense peptide. It is concluded that D-met-enkephalin induced hepatoprotection is comparable to L-met-enkephalin one.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:257223
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-18 10:16:46