prikaz prve stranice dokumenta Mehanizmi rezistencije na β-laktamske antibiotike u gram negativnih bakterija
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doctoral thesis
Mehanizmi rezistencije na β-laktamske antibiotike u gram negativnih bakterija
Zagreb: University of Zagreb, Faculty of Science, 2014. urn:nbn:hr:217:824472
Veir, Zoran
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Veir, Z. (2014). Mehanizmi rezistencije na β-laktamske antibiotike u gram negativnih bakterija (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:824472

Veir, Zoran. "Mehanizmi rezistencije na β-laktamske antibiotike u gram negativnih bakterija." Doctoral thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:824472

Veir, Zoran. "Mehanizmi rezistencije na β-laktamske antibiotike u gram negativnih bakterija." Doctoral thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:824472

Veir, Z. (2014). 'Mehanizmi rezistencije na β-laktamske antibiotike u gram negativnih bakterija', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 09 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:824472

Veir Z. Mehanizmi rezistencije na β-laktamske antibiotike u gram negativnih bakterija [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2014 [cited 2024 November 09] Available at: https://urn.nsk.hr/urn:nbn:hr:217:824472

Z. Veir, "Mehanizmi rezistencije na β-laktamske antibiotike u gram negativnih bakterija", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:217:824472

Metadata
TitleMehanizmi rezistencije na β-laktamske antibiotike u gram negativnih bakterija
Title (english)Mechanisms of resistance to β-lactam antibiotics in gram-negative bacteria
AuthorZoran Veir
MentorNataša Beader (mentor)
MBZ: 178915
Committee memberJasna Hrenović (član povjerenstva)
Committee memberBranka Bedenić (član povjerenstva)
Committee memberMarija Vučemilo (član povjerenstva)
MBZ: 53464
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2014, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
Gram-negativne bakterije su uzročnici teških bolničkih infekcija, a posebice infekcija rana. Postoji stalan trend porasta učestalosti rezistencije na sve veći broj antibakterijskih lijekova koji uz druge činitelje rizika u hospitaliziranih bolesnika doprinose kolonizaciji i nastanku infekcija uzrokovanih multirezistentnim bakterijama. Cilj ovog rada bio je karakterizirati mehanizme rezistencije u izolatima bakterija Acinetobacter baumannii, Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis i Pseudomonas aeruginosa, koje produciraju različite β-laktamaze, iz obrisaka rana. Utvrđena je produkcija β-laktamaza iz TEM, SHV i CTX-M porodice u enterobakteija. U jednom izolatu K. pneumoniae dokazano je prisustvo plazmidne AmpC β-laktamaze iz CMY porodice i VIM metalo-β-laktamaze. Svi izolati P. mirabilis pripadali su istom klonu i posjedovali blaTEM-52 gen. U svih izolata A. baumannii dokazana je insercijska sekvenca ISAba1 i svi su pripadali istom klonu. U izolata P. aeruginosa dokazana je niska stopa produkcije VIM metalo-β-laktamaza i svi su pripadali istom klonu. Niti u jednom izolatu nefermentativnih bakterija nije utvrđena rezistencija na kolistin. Ovi rezultati daju uvid u molekularnu epidemiologiju multirezistentnih bakterija i mogu doprinijeti kreiranju smjernica za empirijsku terapiju teških infekcija.
Abstract (english)
Gram-negative bacteria are considered to be the most important pathogens causing serious nosocomial infections especially wound infections. The increasing resistance to numerous antibiotics together with other risk factors in hospitalized patients are contributing to development of multiresistant bacteria in recent years. The aim of this study was to determine the mechanisms of antibiotic resistance in: Acinetobacter baumannii, Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis and Pseudomonas aeruginosa isolated from wound swabs. ß-lactamases of TEM, SHV and CTX-M family were found in Enterobacteriaceae. Only one isolate of K. pneumoniae produced plasmid-mediated ß-lactamase of CMY family and metallo-β-lactamase of VIM family. All isolates of P. mirabilis harboured blaTEM-52 genes and were clonally related. A. baumannii isolates belonged to a single clone and harboured only blaOXA-51 gene upregulated by ISAba1. The low prevalence of VIM metal-β-lactamase was demonstrated in P. aeruginosa and all of them belonged to the same cluster. Resistance to colistin was not detected in nonfermentative bacteria. The results of this study will give a better insight into molecular epidemiology and enable to establish guidelines for the treatment of these infections.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:824472
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsClosed access
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Created on2017-05-18 11:58:01