Parvovirus B19 is an uncoated single-stranded DNA virus from the Parvoviridae family. This human pathogen primarily infects erythroid progenitors while viral replication occurs in the nucleus of the infected cell. Parvovirus B19 is transmitted through respiratory secretions, blood and blood products as well as vertically, causing fifth disease or erythema infectiosum. Pediatric patients most commonly develop mild symptoms, while pregnant women, immunocompromised individuals and those with hematological disorders may experience more severe clinical manifestations. Based on phylogenetic analysis of the NS1 region of the viral genome, genotypes 1, 2, and 3 are distinguished. Genotypes 1 and 3 are further divided into subtypes A and B. The aim of this study was to determine the distribution of parvovirus B19 genotypes and subtypes in Croatia. The study included 107 participants infected with parvovirus B19, whose blood samples were analyzed at the University Hospital for Infectious Diseases "Dr. Fran Mihaljević" between March and September 2023. The sequences obtained by the Sanger sequencing method showed high phylogenetic similarity and all clustered to genotype 1A. Nucleotide substitutions were detected in six samples, five of which were samesense and one was a missense mutation. The results suggest a common source of infection during the epidemic.