Aquaporin 3 (AQP3) and aquaporin 5 (AQP5) are membrane channels whose role is to facilitate transport of water and other molecules in and out of the cell. They are often overexpressed in numerous cancers, including breast cancer. By transporting molecules like H₂O₂, they affect the redox status in the cell, resulting in oxidative stress and consequently the activation of the transcription factor NRF2. Activated NRF2 has a cytoprotective role in healthy cells, while in tumor cells its excessive activation occurs, which makes the cells resistant to various types of therapy. The aim of this work was to investigate the relation between NRF2 and AQP3 and AQP5 expression. Using the NRF2 activator - sulforaphane and the NRF2 inhibitor - ML385, the effect of NRF2 on the expression of AQP3 and AQP5 was monitored in breast cancer cell lines (positive for estrogen and progesterone receptors - MCF7, positive for HER2 receptor – SkBr3 and triple negative; negative for HER2, estrogen and progesteron receptors - SUM159) and on a non-tumorigenous epithelial breast line (MCF10A). Changes were observed only in MCF7 cell line, where increased expression of hem oxigenase-1 occured after treatment with sulforaphane. In SkBr3 cells, changes were observed in AQP3 expression after treatment with ML385. In other cell lines, there were no significant changes after NRF2 modulation.