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master's thesis
Spolne razlike u oksidativnom i antioksidativnom statusu NOD miševa hranjenih prehranom s visokim udjelom masti
Zagreb: University of Zagreb, Faculty of Science, 2024. urn:nbn:hr:217:028744
Serenčeš, Marina
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Serenčeš, M. (2024). Spolne razlike u oksidativnom i antioksidativnom statusu NOD miševa hranjenih prehranom s visokim udjelom masti (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:028744

Serenčeš, Marina. "Spolne razlike u oksidativnom i antioksidativnom statusu NOD miševa hranjenih prehranom s visokim udjelom masti." Master's thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:028744

Serenčeš, Marina. "Spolne razlike u oksidativnom i antioksidativnom statusu NOD miševa hranjenih prehranom s visokim udjelom masti." Master's thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:028744

Serenčeš, M. (2024). 'Spolne razlike u oksidativnom i antioksidativnom statusu NOD miševa hranjenih prehranom s visokim udjelom masti', Master's thesis, University of Zagreb, Faculty of Science, accessed 09 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:028744

Serenčeš M. Spolne razlike u oksidativnom i antioksidativnom statusu NOD miševa hranjenih prehranom s visokim udjelom masti [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2024 [cited 2024 November 09] Available at: https://urn.nsk.hr/urn:nbn:hr:217:028744

M. Serenčeš, "Spolne razlike u oksidativnom i antioksidativnom statusu NOD miševa hranjenih prehranom s visokim udjelom masti", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:217:028744

Metadata
TitleSpolne razlike u oksidativnom i antioksidativnom statusu NOD miševa hranjenih prehranom s visokim udjelom masti
Title (english)Sex-related differences in oxidative and antioxidative status of NOD mice fed with high-fat diet
AuthorMarina Serenčeš
MentorRobert Belužić (mentor)
Committee memberMorana Dulić (predsjednik povjerenstva)
Committee memberIvan Nemet (član povjerenstva)
Committee memberMirta Rubčić (član povjerenstva)
Committee memberIta Gruić Sovulj (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2024-07-26, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Dijabetes je bolest u kojoj dolazi do poremećaja u dostavi inzulina ili općeg nedostatka inzulina u organizmu. Dobar model za proučavanje svih poremećaja u metaboličkoj regulaciji, oksidativnom stresu i mitohondrijskoj obrani su NOD (engl. non-obese diabetic) miševi koji spontano razvijaju dijabetes. Kod ovog soja miševa dokazane su razlike u osjetljivosti na razvijanje dijabetesa ovisno o spolu, što je ključno u razvoju terapije prilikom liječenja. U ovom radu je promatran utjecaj standardne prehrane (engl. standard fat diet, SFD) i prehrane s visokim udjelom masti (engl. high-fat diet, HFD) na metaboličke promjene uzrokovane prehranom na oksidacijsko oštećenje, antioksidacijsku obranu i ekspresiju ključnih proteina u metabolizmu lipida i mitohondrijske funkcije u uzorcima jetri ženki i mužjaka NOD miševa. Promatrana je karbonilacija proteina, razina lipidne peroksidacije, aktivnost superoksid‒dismutaze i aktivnost katalaze. Western analizom određena je ekspresija antioksidativnih enzima i proteina uključenih u regulaciju metabolizma.
Abstract (english)
Diabetes is a disease in which there is a disturbance in the delivery of insulin or a general lack of insulin in the body. A good model for studying all disorders in metabolic regulation, oxidative stress and mitochondrial defense are NOD mice (non-obese diabetic), which spontaneously develop diabetes. In this strain of mice, sex-sensitive differences in susceptibility to developing diabetes have been demonstrated, which is crucial for the development of appropriate therapy in treatment. In this paper, the influence of standard diet (SFD) and high-fat diet (HFD) on metabolic changes caused by diet on oxidative damage, antioxidant defense and expression of key proteins in lipid metabolism and mitochondrial function in liver samples of female and male NOD mice was observed. Protein carbonylation, lipid peroxidation level, superoxide dismutase activity and catalase activity were observed. Western blot was used to determine the expression of antioxidant enzymes and proteins involved in the regulation of metabolism.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:028744
Study programmeTitle: Graduate university studies in chemistry; specializations in: Graduate university studies in chemistry
Course: Graduate university studies in chemistry
Study programme type: university
Study level: graduate
Academic / professional title: sveučilišni/a magistar/magistra kemije (sveučilišni/a magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-10-03 14:13:02