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master's thesis
Simetrični poliini izvedeni iz bis-triarilamina kao fluorescentni senzori za DNA i RNA
Zagreb: University of Zagreb, Faculty of Science, 2024. urn:nbn:hr:217:725000
Vukadinović, Nina
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Vukadinović, N. (2024). Simetrični poliini izvedeni iz bis-triarilamina kao fluorescentni senzori za DNA i RNA (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:725000

Vukadinović, Nina. "Simetrični poliini izvedeni iz bis-triarilamina kao fluorescentni senzori za DNA i RNA." Master's thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:725000

Vukadinović, Nina. "Simetrični poliini izvedeni iz bis-triarilamina kao fluorescentni senzori za DNA i RNA." Master's thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:725000

Vukadinović, N. (2024). 'Simetrični poliini izvedeni iz bis-triarilamina kao fluorescentni senzori za DNA i RNA', Master's thesis, University of Zagreb, Faculty of Science, accessed 26 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:725000

Vukadinović N. Simetrični poliini izvedeni iz bis-triarilamina kao fluorescentni senzori za DNA i RNA [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2024 [cited 2024 November 26] Available at: https://urn.nsk.hr/urn:nbn:hr:217:725000

N. Vukadinović, "Simetrični poliini izvedeni iz bis-triarilamina kao fluorescentni senzori za DNA i RNA", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:217:725000

Metadata
TitleSimetrični poliini izvedeni iz bis-triarilamina kao fluorescentni senzori za DNA i RNA
Title (english)Symmetrical polyynes derived from bis-triarylamine as fluorescence sensor for DNA and RNA
AuthorNina Vukadinović
MentorDijana Pavlović Saftić (mentor)
Committee memberIva Juranović Cindrić (predsjednik povjerenstva)
Committee memberĐani Škalamera (član povjerenstva)
Committee memberMirta Rubčić (član povjerenstva)
Committee memberIvan Nemet (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2024-10-16, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Fluorescentne probe za analitičke svrhe su ključne za napredak u gotovo svim područjima prirodnih znanosti, a vrlo često pronalaze primjenu u specifičnoj detekciji polinukleotida. U okviru ovog rada provedena je spektroskopska karakterizacija pet spojeva izvedenih iz bis-triarilamina primjenom UV/Vis i fluorescencijske spektroskopije. Ispitan je utjecaj supstituenata na afinitet i način vezanja na prirodnu DNA te sintetske molekule DNA i RNA. Obradom fluorimetrijskih titracija pomoću Scatchardove jednadžbe metodom nelinearne regresijske analize dobivene su konstante stabilnosti kompleksa proba s polinukleotidima. Eksperimenti temperaturnog mekšanja polinukleotida i titracije metodom cirkularnog dikroizma su pokazali visok afinitet spojeva prema polinukleotidima, bez značajne selektivnosti prema specifičnim sekvencama. Dodatak ovih spojeva dvolančanim molekulama obično je rezultirao slabijom (de)stabilizacijom heliksa, osim jednog spoja koji je blago stabilizirao određeni tip DNA. Dobiveni rezultati ukazuju na potencijal ovih spojeva kao fluorescentnih senzora u biološkim istraživanjima.
Abstract (english)
Fluorescent probes for analytical purposes are crucial for advancements in nearly all fields of natural sciences and are often used for the specific detection of polynucleotides. This study involved the spectroscopic characterization of five compounds derived from bis-triarylamines using UV/Vis and fluorescence spectroscopy. The influence of substituents on the affinity and binding mode to natural DNA and synthetic DNA and RNA molecules was investigated. Analysis of fluorimetric titrations using the Scatchard equation with nonlinear regression methods provided stability constants for the complexes with polynucleotides. Experiments on the thermal melting of polynucleotides and circular dichroism titrations demonstrated a high affinity of the compounds for polynucleotides, with no significant selectivity towards specific sequences. The addition of these compounds to double-stranded molecules generally resulted in weaker stabilization of the helix, except for one compound, which slightly stabilized a specific type of DNA. The results indicate the potential of these compounds as fluorescent sensors in biological research.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:725000
ProjectNumber: IP-2022-10-9829
Title: Novi peptid-oligonukleotid-kromofor konjugati za biokemijsku dijagnostiku i bioaktivnost
Title: New peptide-oligonucleotide-chromophore conjugates for biochemical diagnostics and bioactivity
Acronym: POC-Sens-Bioact
Leader: Ivo Piantanida
Jurisdiction: Croatia
Funder: Hrvatska zaklada za znanost
Funding stream: Research Projects
Study programmeTitle: Graduate university studies in chemistry; specializations in: Graduate university studies in chemistry
Course: Graduate university studies in chemistry
Study programme type: university
Study level: graduate
Academic / professional title: sveučilišni/a magistar/magistra kemije (sveučilišni/a magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-10-24 12:52:07