prikaz prve stranice dokumenta ANALIZA PROTEINSKOG PROFILA U STANICAMA INTRAEPITELNIH LEZIJA VRATA MATERNICE
  Download
PDF 4.19 MB
doctoral thesis
ANALIZA PROTEINSKOG PROFILA U STANICAMA INTRAEPITELNIH LEZIJA VRATA MATERNICE
Zagreb: University of Zagreb, Faculty of Science, 2024. urn:nbn:hr:217:239709
Pešut, Ena
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Pešut, E. (2024). ANALIZA PROTEINSKOG PROFILA U STANICAMA INTRAEPITELNIH LEZIJA VRATA MATERNICE (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:239709

Pešut, Ena. "ANALIZA PROTEINSKOG PROFILA U STANICAMA INTRAEPITELNIH LEZIJA VRATA MATERNICE." Doctoral thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:239709

Pešut, Ena. "ANALIZA PROTEINSKOG PROFILA U STANICAMA INTRAEPITELNIH LEZIJA VRATA MATERNICE." Doctoral thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:239709

Pešut, E. (2024). 'ANALIZA PROTEINSKOG PROFILA U STANICAMA INTRAEPITELNIH LEZIJA VRATA MATERNICE', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 26 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:239709

Pešut E. ANALIZA PROTEINSKOG PROFILA U STANICAMA INTRAEPITELNIH LEZIJA VRATA MATERNICE [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2024 [cited 2024 December 26] Available at: https://urn.nsk.hr/urn:nbn:hr:217:239709

E. Pešut, "ANALIZA PROTEINSKOG PROFILA U STANICAMA INTRAEPITELNIH LEZIJA VRATA MATERNICE", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:217:239709

Metadata
TitleANALIZA PROTEINSKOG PROFILA U STANICAMA INTRAEPITELNIH LEZIJA VRATA MATERNICE
Title (english)PROTEIN PROFILE ANALYSIS OF CELLS FROM INTRAEPITHELIAL CERVICAL LESIONS
AuthorEna Pešut
MentorIvan Sabol (mentor)
Committee memberMaja Matulić (predsjednik povjerenstva)
Committee memberRosa Karlić (član povjerenstva)
Committee memberRajko Fureš (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2024-10-21, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
Rak vrata maternice četvrti je po učestalosti i smrtnosti u svijetu, a prethode mu prekancerozne intraepitelne lezije povezane s infekcijom ljudskim papiloma virusom. Cilj ovog istraživanja bio je analizirati proteinski profil stanica ovisno o stupnju prekancerozne intraepitelne lezije i prisutnosti ljudskog papiloma virusa. U ovom istraživanju je na brisevima vrata maternice s prekanceroznim intraepitelnim lezijama utvrđena prisutnost ljudskog papiloma virusa lančanom reakcijom polimerazom, a proteinski profil masenom citometrijom i imunocitokemijom. Na bioptatima vrata maternice utvrđen je proteinski profil imunohistokemijom. Rezultati su pokazali da je masena citometrija prikladna za uspješnu analizu proteinskog profila na brisevima vrata maternice. Ekspresija proteina Ck18, Ck8, CD63, CD71, KLF4, LRIG1, E-kadherin, Nanog, p53, Ck17, MCM2, MMP7 i CD29 razlikovala se s obzirom na težinu stupnja prekancerozne lezije i prisutnost HPV-a.
Abstract (english)
Cervical cancer is the fourth most common cancer in the world by incidence and mortality, and is preceded by precancerous intraepithelial lesions associated with human papillomavirus (HPV) infection. The aim of this study was to analyze the protein profile of cells depending on the stage of the precancerous intraepithelial lesion and the presence of HPV. In this study, the presence of HPV was determined on cervical swabs with precancerous intraepithelial lesions by polymerase chain reaction, and the protein profile was established by mass cytometry and immunocytochemistry. The protein profile of cervical biopsies was determined by immunohistochemistry. The results showed that mass cytometry was suitable for the successful analysis of the protein profile on cervical swabs. The expression of proteins Ck18, Ck8, CD63, CD71, KLF4, LRIG1, E-cadherin, Nanog, p53, Ck17, MCM2, MMP7, and CD29 differed with regard to the severity of the precancerous lesion and the presence of HPV.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:239709
Promotion2024
ProjectNumber: IP-2019-04-3403
Title: Multifaktorna analiza HPV inficiranih stanica u nastanku raka vrata maternice
Title: Multifactor analysis of HPV infected cells in cervical cancer development
Acronym: MACCD
Leader: Ivan Sabol
Jurisdiction: Croatia
Funder: Hrvatska zaklada za znanost
Funding stream: Research Projects
Study programmeTitle: Doctoral study
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-12-02 12:04:41