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doctoral thesis
THE ROLES OF AUGMIN AND SPINDLE ACTIN IN CHROMOSOME CONGRESSION, SPINDLE ARCHITECTURE AND MITOTIC FIDELITY
Zagreb: University of Zagreb, Faculty of Science, 2024. urn:nbn:hr:217:130520
Štimac, Valentina
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Štimac, V. (2024). THE ROLES OF AUGMIN AND SPINDLE ACTIN IN CHROMOSOME CONGRESSION, SPINDLE ARCHITECTURE AND MITOTIC FIDELITY (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:130520

Štimac, Valentina. "THE ROLES OF AUGMIN AND SPINDLE ACTIN IN CHROMOSOME CONGRESSION, SPINDLE ARCHITECTURE AND MITOTIC FIDELITY." Doctoral thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:130520

Štimac, Valentina. "THE ROLES OF AUGMIN AND SPINDLE ACTIN IN CHROMOSOME CONGRESSION, SPINDLE ARCHITECTURE AND MITOTIC FIDELITY." Doctoral thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:130520

Štimac, V. (2024). 'THE ROLES OF AUGMIN AND SPINDLE ACTIN IN CHROMOSOME CONGRESSION, SPINDLE ARCHITECTURE AND MITOTIC FIDELITY', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 12 March 2025, https://urn.nsk.hr/urn:nbn:hr:217:130520

Štimac V. THE ROLES OF AUGMIN AND SPINDLE ACTIN IN CHROMOSOME CONGRESSION, SPINDLE ARCHITECTURE AND MITOTIC FIDELITY [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2024 [cited 2025 March 12] Available at: https://urn.nsk.hr/urn:nbn:hr:217:130520

V. Štimac, "THE ROLES OF AUGMIN AND SPINDLE ACTIN IN CHROMOSOME CONGRESSION, SPINDLE ARCHITECTURE AND MITOTIC FIDELITY", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:217:130520

Metadata
TitleTHE ROLES OF AUGMIN AND SPINDLE ACTIN IN CHROMOSOME CONGRESSION, SPINDLE ARCHITECTURE AND MITOTIC FIDELITY
Title (croatian)ULOGE AUGMINA I AKTINA UNUTAR DIOBENOG VRETENA U KONGRESIJI KROMOSOMA, ARHITEKTURI VRETENA I VJERNOSTI MITOZE
AuthorValentina Štimac
MentorIva Marija Tolić (mentor)
Committee memberAndreja Ambriović Ristov (predsjednik povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2024-10-23, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		576 - Cellular and subcellular biology. Cytology
Abstract
The human mitotic spindle is made of microtubules nucleated at centrosomes, at kinetochores, and from pre-existing microtubules by the augmin complex. Recently, spindle actin also emerged as a possible player in spindle assembly. However, it is unknown how augmin-mediated nucleation affects distinct microtubule classes, what the spatial distribution of spindle actin and microtubules is, and, finally, what the roles of these two proteins are in spindle architecture and mitotic progression. Here,... More by using superresolution microscopy and cutting edge live-cell imaging, I found that augmin promotes mitotic fidelity by generating assemblies consisting of bridging and kinetochore fibers that align sister kinetochores to face opposite poles, thereby preventing erroneous attachments. Furthermore, I showed that spindle actin, by forming fine filaments in the polar region and along spindle microtubules, promotes spindle pole focusing and bipolar cell division. Less
Abstract (croatian)
Mikrotubuli diobenog vretena se u ljudskim stanicama nukleiraju na centrosomu, kinetohorama te na površini već postojećih mikrotubula pomoću augminskog kompleksa. Nedavno je predloženo da i aktin unutar diobenog vretena ima ulogu u njegovom sastavljanju. Međutim, još uvijek nije poznato na koji način nukleacija pomoću augmina utječe na različite klase mikrotubula, koja je prostorna raspodjela aktina i mikrotubula unutar vretena te koja je uloga ova dva proteina u arhitekturi vretena i... More progresiji mitoze. Koristeći superrezolucijsku mikroskopiju te najnoviju tehnologiju snimanja živih stanica pokazala sam da augmin ima ulogu u pravilnoj podjeli kromosoma. Augmin stvara sklopove mikrotubula koji se sastoje od premošćujućih i kinetohornih vlakana što poravnava sestrinske kinetohore na način da su okrenute prema suprotnim polovima, te tako sprječava pogrešna vezanja s mikrotubulima. Nadalje, pokazala sam da aktin unutar vretena promiče fokusiranje polova vretena te bipolarnu mitozu tako što formira filamente u polarnoj regiji te uzduž mikrotubula. Less
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:217:130520
Promotion2024
ProjectNumber: PZS-2019-02-7653
Title: Mehanizmi nastajanja snopova mikrotubula potrebni za sazrijevanje diobenog vretena
Title: Mechanisms of microtubule bundle formation required for spindle maturation
Acronym: SpindleForm
Leader: Iva Marija Tolić
Jurisdiction: Croatia
Funder: Hrvatska zaklada za znanost
Funding stream: Programme Research Cooperability
Study programmeTitle: Doctoral study
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-12-02 18:11:40
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