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master's thesis
Utjecaj statina na stanične linije karcinoma prostate čovjeka u 2D i 3D uvjetima rasta in vitro
Zagreb: University of Zagreb, Faculty of Science, 2025. urn:nbn:hr:217:059309
Uhrl, Klara
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Uhrl, K. (2025). Utjecaj statina na stanične linije karcinoma prostate čovjeka u 2D i 3D uvjetima rasta in vitro (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:059309

Uhrl, Klara. "Utjecaj statina na stanične linije karcinoma prostate čovjeka u 2D i 3D uvjetima rasta in vitro." Master's thesis, University of Zagreb, Faculty of Science, 2025. https://urn.nsk.hr/urn:nbn:hr:217:059309

Uhrl, Klara. "Utjecaj statina na stanične linije karcinoma prostate čovjeka u 2D i 3D uvjetima rasta in vitro." Master's thesis, University of Zagreb, Faculty of Science, 2025. https://urn.nsk.hr/urn:nbn:hr:217:059309

Uhrl, K. (2025). 'Utjecaj statina na stanične linije karcinoma prostate čovjeka u 2D i 3D uvjetima rasta in vitro', Master's thesis, University of Zagreb, Faculty of Science, accessed 12 March 2025, https://urn.nsk.hr/urn:nbn:hr:217:059309

Uhrl K. Utjecaj statina na stanične linije karcinoma prostate čovjeka u 2D i 3D uvjetima rasta in vitro [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2025 [cited 2025 March 12] Available at: https://urn.nsk.hr/urn:nbn:hr:217:059309

K. Uhrl, "Utjecaj statina na stanične linije karcinoma prostate čovjeka u 2D i 3D uvjetima rasta in vitro", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2025. Available at: https://urn.nsk.hr/urn:nbn:hr:217:059309

Metadata
TitleUtjecaj statina na stanične linije karcinoma prostate čovjeka u 2D i 3D uvjetima rasta in vitro
Title (english)Effect of statins on human prostate cancer cell lines in 2D and 3D growth conditions in vitro
AuthorKlara Uhrl
MentorMaja Sabol (mentor)
MentorNada Oršolić (komentor)
Committee memberNada Oršolić (predsjednik povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
Committee memberDomagoj Đikić (član povjerenstva)
Committee memberInga Urlić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2025, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Statini su inhibitori 3-hidroksi-3-metilglutaril koenzim A reduktaze (HMGCR) koji blokirajući aktivnost ovog enzima smanjuju razinu kolesterola u stanicama. S obzirom na svoj reducirajući učinak na kolesterol, statini se uglavnom koriste za liječenje kardiovaskularnih bolesti. Usprkos svojoj širokoj primjeni u liječenju bolesti krvožilnog sustava, brojna pretklinička istraživanja dokazala su antineoplastični učinak statina na neke vrste tumora poput kolorektalnog karcinoma ili karcinoma... More prostate (PCa). U ovom radu ispitivali smo učinak simvastatina i lovastatina na stanice karcinoma prostate (LNCaP i PC3) te fibroblasta prostate (WPMY-1) u dvodimenzionalnim i trodimenzionalnim uvjetima rasta. Tri stanične linije pokazuju različitu osjetljivost na statine, pri čemu su LNCaP stanice najotpornije u oba uvjeta rasta. Oba statina smanjuju ekspresiju AR (gen za androgeni receptor), PSA (antigen specifičan za prostatu) i KLK2 (kalikrein 2) gena u 2D i 3D staničnim kulturama. Stanice uzgojene u trodimenzionalnim uvjetima pokazuju slabiji ili čak suprotan odgovor na tretmane što ukazuje na rezistenciju na ove spojeve. Less
Abstract (english)
Statins are inhibitors of 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase (HMGCR) which, by blocking the activity of this enzyme, reduce the level of cholesterol in the cells. Due to their cholesterol-lowering effect, statins are mainly used to treat cardiovascular diseases. Despite their wide application in treatment of vascular diseases, numerous preclinical studies found antineoplastic effect of statins on different tumors such as colon or prostate cancer. In this work, we... More investigated the effect of simvastatin and lovastatin on the prostate cancer cells (LNCaP and PC3) and prostate fibroblasts (WPMY-1) in two dimensional and three dimensional growth conditions. The three cell lines show different sensitivity to statins, with LNCaP cells being the most resistant in both growth conditions. Both statins downregulate the expression of androgen receptor gene (AR), prostate specific antigen (PSA) and kallikrein related peptidase 2 (KLK2) in two dimensional and three dimensional cell cultures. Cells grown in three dimensional conditions show weaker or even opposite response to treatments indicating the resistance to these compounds. Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:059309
ProjectNumber: IP-2022-10-6672
Title: Uloga signalnog puta Hedgehog-GLI u komunikaciji tumora i strome posredovanoj faktorima rasta u 2D i 3D tumorskim modelima
Title: The role of Hedgehog-GLI signaling in growth factor-mediated tumor-stroma communication in 2D and 3D in vitro tumor models
Acronym: HHgrow3D
Leader: Maja Sabol
Jurisdiction: Croatia
Funder: Hrvatska zaklada za znanost
Funding stream: Research Projects
Study programmeTitle: Experimental Biology-University graduate programme
Study programme type: university
Study level: graduate
Academic / professional title: sveučilišni/a magistar/magistra eksperimentalne biologije (sveučilišni/a magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2025-01-27 13:19:11
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