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master's thesis
Učinak mikrosatelitske nestabilnosti i somatskih mutacija gena KRAS i PIK3CA na progresiju raka želuca čovjeka
Zagreb: University of Zagreb, Faculty of Science, 2024. urn:nbn:hr:217:347944
Crnjac, Iva
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Crnjac, I. (2024). Učinak mikrosatelitske nestabilnosti i somatskih mutacija gena KRAS i PIK3CA na progresiju raka želuca čovjeka (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:347944

Crnjac, Iva. "Učinak mikrosatelitske nestabilnosti i somatskih mutacija gena KRAS i PIK3CA na progresiju raka želuca čovjeka." Master's thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:347944

Crnjac, Iva. "Učinak mikrosatelitske nestabilnosti i somatskih mutacija gena KRAS i PIK3CA na progresiju raka želuca čovjeka." Master's thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:347944

Crnjac, I. (2024). 'Učinak mikrosatelitske nestabilnosti i somatskih mutacija gena KRAS i PIK3CA na progresiju raka želuca čovjeka', Master's thesis, University of Zagreb, Faculty of Science, accessed 12 March 2025, https://urn.nsk.hr/urn:nbn:hr:217:347944

Crnjac I. Učinak mikrosatelitske nestabilnosti i somatskih mutacija gena KRAS i PIK3CA na progresiju raka želuca čovjeka [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2024 [cited 2025 March 12] Available at: https://urn.nsk.hr/urn:nbn:hr:217:347944

I. Crnjac, "Učinak mikrosatelitske nestabilnosti i somatskih mutacija gena KRAS i PIK3CA na progresiju raka želuca čovjeka", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:217:347944

Metadata
TitleUčinak mikrosatelitske nestabilnosti i somatskih mutacija gena KRAS i PIK3CA na progresiju raka želuca čovjeka
Title (english)The impact of microsatellite instability and somatic mutations of KRAS and PIK3CA genes on the progression of human gastric cancer
AuthorIva Crnjac
MentorSnježana Ramić (mentor)
MentorNada Oršolić (komentor)
Committee memberNada Oršolić (predsjednik povjerenstva)
Committee memberRomana Gračan (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
Committee memberDomagoj Đikić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2024-09-24, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Rak želuca je 2020. godine bio peti najčešći oblik raka te četvrti po smrtnosti na globalnoj razini, sve češće pogađajući osobe mlađe od 50 godina. Karakteriziraju ga mnoge promjene među kojima su i mikrosatelitska nestabilnost (MSI) te mutacije gena KRAS i PIK3CA koje su česte i u drugih tipova tumora. Cilj ovog rada bio je istražiti imaju li na navedene promjene učinak na progresiju raka želuca čovjeka. U 69 uzoraka raka želuca analizirana je prisutnost mutacija gena KRAS (11,6 %) i PIK3CA... More (7,3 %) lančanom reakcijom polimeraze, a imunohistokemijskim bojenjem mikrosatelitska nestabilnost uzoraka (MSI) (31,9 %). Provedenim istraživanjem utvrđena je statistički značajna korelacija veličine tumora sa statusom MSI (rs = –0,283, p = 0,020) i prisutnim mutacijama gena KRAS (rs = –0,311, p = 0,010). Ovo može upućivati na to da su te promjene rani događaj u karcinogenezi koje imaju pokretački karakter (engl. driver), zbog čega dolazi do razvoja drugačijeg molekularnog profila raka želuca. Nije utvrđen utjecaj statusa MSI ili mutacija u genima KRAS i PIK3CA na progresiju raka želuca. U tom smjeru potrebno je provesti dodatna istraživanja na većem broju uzoraka. Less
Abstract (english)
In 2020, gastric cancer was the fifth most common type of cancer and the fourth leading cause of cancer-related mortality globally, increasingly affecting individuals under the age of 50. This malignancy is characterized by various alterations, including microsatellite instability (MSI) and mutations in the KRAS and PIK3CA genes, which are also frequently observed in other tumor types. The aim of this study was to investigate whether these alterations have an impact on the progression of... More human gastric cancer. In 69 gastric cancer samples, the presence of KRAS (11.6 %) and PIK3CA (7.3 %) gene mutations was analyzed using polymerase chain reaction (PCR), while microsatellite instability (MSI) was assessed by immunohistochemical staining (31,9 %). The study identified a statistically significant correlation between tumor size and MSI status (rs = –0.283, p = 0.020), as well as between tumor size and the presence of KRAS gene mutations (rs = –0.311, p = 0.010). This may suggest that these alterations are early events in carcinogenesis with a driver nature, leading to the development of a distinct molecular profile in gastric cancer. No impact of MSI status or mutations in the KRAS and PIK3CA genes on the progression of gastric cancer was observed. Further research in this direction is required, involving a larger number of samples. Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:347944
Study programmeTitle: Experimental Biology-University graduate programme
Study programme type: university
Study level: graduate
Academic / professional title: sveučilišni/a magistar/magistra eksperimentalne biologije (sveučilišni/a magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2025-01-29 14:57:24
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