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doctoral thesis
UTJECAJ SPECIJACIJE METALA NA TOKSIČNOST NANOČESTICA BAKRA U ODABRANIM VRSTAMA MIKROALGA, DAGNJA I JEŽINACA
Zagreb: University of Zagreb, Faculty of Science, 2024. urn:nbn:hr:217:663141
Čarapar, Ivana
University of Zagreb
Faculty of Science
Department of Geology

Institutional repository: Repository of the Faculty of Science

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Čarapar, I. (2024). UTJECAJ SPECIJACIJE METALA NA TOKSIČNOST NANOČESTICA BAKRA U ODABRANIM VRSTAMA MIKROALGA, DAGNJA I JEŽINACA (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:663141

Čarapar, Ivana. "UTJECAJ SPECIJACIJE METALA NA TOKSIČNOST NANOČESTICA BAKRA U ODABRANIM VRSTAMA MIKROALGA, DAGNJA I JEŽINACA." Doctoral thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:663141

Čarapar, Ivana. "UTJECAJ SPECIJACIJE METALA NA TOKSIČNOST NANOČESTICA BAKRA U ODABRANIM VRSTAMA MIKROALGA, DAGNJA I JEŽINACA." Doctoral thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:663141

Čarapar, I. (2024). 'UTJECAJ SPECIJACIJE METALA NA TOKSIČNOST NANOČESTICA BAKRA U ODABRANIM VRSTAMA MIKROALGA, DAGNJA I JEŽINACA', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 28 March 2025, https://urn.nsk.hr/urn:nbn:hr:217:663141

Čarapar I. UTJECAJ SPECIJACIJE METALA NA TOKSIČNOST NANOČESTICA BAKRA U ODABRANIM VRSTAMA MIKROALGA, DAGNJA I JEŽINACA [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2024 [cited 2025 March 28] Available at: https://urn.nsk.hr/urn:nbn:hr:217:663141

I. Čarapar, "UTJECAJ SPECIJACIJE METALA NA TOKSIČNOST NANOČESTICA BAKRA U ODABRANIM VRSTAMA MIKROALGA, DAGNJA I JEŽINACA", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:217:663141

Metadata
TitleUTJECAJ SPECIJACIJE METALA NA TOKSIČNOST NANOČESTICA BAKRA U ODABRANIM VRSTAMA MIKROALGA, DAGNJA I JEŽINACA
Title (english)INFLUENCE OF METAL SPECIATION ON TOXICITY OF COPPER–BASED NANOPARTICLES IN SELECTED MICROALGA, MUSSEL AND URCHIN SPECIES
AuthorIvana Čarapar
MentorDaniel Mark Lyons (mentor)
Committee memberBiljana Balen (predsjednik povjerenstva)
Committee memberDario Omanović (član povjerenstva)
Committee memberDijana Pavičić-Hamer (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Geology)
Zagreb
Defense date and country2024-10-29, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Interdisciplinary Natural Sciences
Marine Science
Universal decimal classification
(UDC)		55 - Geology. Meteorology. Hydrology
Abstract
Nanočestice (eng. nanoparticles, NP) bakra upotrebljavaju se kao antimikrobni aditivi u tekstilnoj industriji, tehnologiji pročišćavanja vode i premazima protiv obraštaja. Upravo upotreba u bojama predstavlja izravniji put za ispuštanje u morski okoliš, zbog degradacije boje u brodskim premazima tijekom vremena gdje mogu utjecati na širok raspon organizama u svim životnim fazama, od embrija do odraslih jedinki. Cilj ovog rada bio je istražiti utjecaj specijacije nanočestica bakra (CuNP),... More bakrovog (II) oksida (CuONP) i bakrovog (I) oksida (Cu2ONP) i kako abiotički čimbenici (svjetlosna jakost, salinitet i pH vode) utječu na stabilnost NP. Potencijalni toksični učinak specijacije NP ispitan je na morskim organizmima: mikroalgi Dunaliella salina, dagnji Mytilus galloprovincialis te ježincima Arbacia lixula, Paracentrous lividus i Sphaerechinus granularis. Na razini organizma pratila se fotosintetska učinkovitost kod mikroalge, test stres na stres kod dagnje te test spermiotoksičnosti i embriotoksičnosti kod ježinaca, gdje je ispitan negativan utjecaj ovisno o koncentraciji navedenih nanočestica. Na staničnoj razini kod mikroalge istražena je lipidna peroksidacija te aktivnost enzima glutation S–transferaze (GST), katalaze (CAT) i superoksid dismutaze (SOD). Kod dagnje je određena i razina metalotioneina (MT) u probavnoj žlijezdi te aktivnost acetikolinesteraze (AChE), a kod ježinaca test citogenotoksičnosti. Ovi testovi su pokazali korelaciju između razine oksidativnog stresa, aktivacije obrambenih mehanizama i fizikalno-kemijskih karakteristika NP. Općenito, ovaj rad je pokazao kako su Cu2ONP najtoksičnije za sve ispitane organizme, što može imati posljedice u svim razvojnim fazama budući da je to oblik NP bakra koji se naširoko koristi u premazima protiv obraštaja. Less
Abstract (english)
Copper nanoparticles (NP) are used as antimicrobial additives in the textile industry, water purification technology and anti-fouling coatings. It is their use in such coatings that represents a more direct route for their release into the marine environment where, due to the degradation of the coatings over time, they can affect a wide range of non-target organisms in all life stages, from embryos to adults. The aim of this doctoral thesis was to investigate the influence of speciation... More of copper (CuNP), copper (II) oxide (CuONP) and copper (I) oxide (Cu2ONP) and how abiotic factors such as light intensity, salinity and water pH can effect the stability of NP. The potential toxicity of these materials was tested on selected marine organisms: microalgae Dunaliella salina, mussels Mytilus galloprovincialis and sea urchins Arbacia lixula, Paracentrous lividus and Sphaerechinus granularis. At the whole organism level, photosynthetic efficiency was monitored in D. salina, physiological state in M. galloprovincialis, and impact on fertilisation ability, transmissible damage to offspring and developmental anomalies in the sea urchins, where a negative influence was shown depending on the NP concentration. At the cellular level, lipid peroxidation and the activity of glutathione S-transferase (GST), catalase (CAT) and superoxide dismutase (SOD) enzymes in microalgae D. salina were determined. Additionally, in M. galloprovincialis, the level of induced metallothionein in the digestive gland and the activity of acetylcholinesterase (AChE) in the gills were determined, while in sea urchins cytogenotoxicity was determined. These tests showed correlation between the level of oxidative stress, the activation of defense mechanisms and the physicochemical properties of NP. Overall, this study demonstrated that Cu2ONP were the most toxic to all organisms, which can have consequences in all developmental stages as this is the form of copper NP widely used in anti-fouling coatings. Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:663141
Promotion2025
ProjectNumber: IP-2018-01-5351
Title: Određivanje fizikalno-kemijskih svojstava i toksičnosti nanočestica srebra, bakra i plastike kao potencijalno štetnih novih materijala u obalnim vodama
Acronym: NANO-EMC2
Leader: Daniel Mark Lyons
Jurisdiction: Croatia
Funder: Hrvatska zaklada za znanost
Funding stream: Research Projects
Study programmeTitle: DOCTORAL STUDY OF OCEANOLOGY
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
Type of resourceText
Extent152 str. ; 30 cm
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2025-01-30 08:16:08
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