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doctoral thesis
Rane imunoreakcije u mononuklearnim stanicama periferne krvi uzrokovane virusom puumala
Zagreb: University of Zagreb, Faculty of Science, 2014. urn:nbn:hr:217:832620
Cvetko Krajinović, Lidija
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Cvetko Krajinović, L. (2014). Rane imunoreakcije u mononuklearnim stanicama periferne krvi uzrokovane virusom puumala (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:832620

Cvetko Krajinović, Lidija. "Rane imunoreakcije u mononuklearnim stanicama periferne krvi uzrokovane virusom puumala." Doctoral thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:832620

Cvetko Krajinović, Lidija. "Rane imunoreakcije u mononuklearnim stanicama periferne krvi uzrokovane virusom puumala." Doctoral thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:832620

Cvetko Krajinović, L. (2014). 'Rane imunoreakcije u mononuklearnim stanicama periferne krvi uzrokovane virusom puumala', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:832620

Cvetko Krajinović L. Rane imunoreakcije u mononuklearnim stanicama periferne krvi uzrokovane virusom puumala [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2014 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:832620

L. Cvetko Krajinović, "Rane imunoreakcije u mononuklearnim stanicama periferne krvi uzrokovane virusom puumala", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:217:832620

Metadata
TitleRane imunoreakcije u mononuklearnim stanicama periferne krvi uzrokovane virusom puumala
Title (english)Early immunoreactions in peripheral blood mononuclear cells induced by puumala virus
AuthorLidija Cvetko Krajinović
MentorAlemka Markotić (mentor)
Committee memberSnježana Židovec Lepej (član povjerenstva)
Committee memberDubravko Forčić (član povjerenstva)
Committee memberMirna Ćurković Perica (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2014, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
Cilj ovoga istraživanja bio je istražiti komponente urođene i stečene imunosti ključne u ranom perifernom imunološkom odgovoru na hantavirusnu infekciju kao i mogući regulacijski učinak miRNA na periferni imunološki odgovor. U mononuklearnim stanicama periferne krvi bolesnika s hemoragijskom vrućicom s bubrežnim sindromom (HVBS) uzrokovanim virusom Puumala (PUUV) određivana je relativna ekspresija gena urođene i stečene imunosti kao i miRNA, tehnologijom real-time PCR array-a. Rezultati su pokazali supresiju ranog imunološkog odgovora na PUUV, tj. ekspresije gena koji kodiraju sintezu receptora za prepoznavanje obrazaca mikroorganizama, kemokina i kemokinskih receptora, drugih citokina, transkripcijskih faktora kao i pojedinih signalnih molekula. Funkcionalne analize pokazale su da supresija ekspresije opisane skupine gena modulira upalni odgovor stanica putem interferencije s različitim staničnim signalnim putovima. Po prvi je puta pokazana biološka značajnost miRNA u regulaciji imunološkog odgovora tijekom HVBS-a. Detektirane promjene na razini imunološkog odgovora mogu se dovesti u korelaciju s težinom bolesti, ali ne i s količinom virusa u krvi. MAPK8, CCR5, IL-10, STAT1, STAT4, miR21 i miR19a mogli bi biti potencijalni prediktori patogeneze HVBS-a.
Abstract (english)
The aim of this study was to explore the components of innate and adaptive immunity important in the early peripheral immune response to hantaviral infection as well as possible regulatory effect of miRNA to peripheral immune response. Using real-time PCR array technology, the relative expression of immune response genes and miRNA was measured in peripheral blood mononuclear cells of patients with hemorrhagic fever with renal syndrome (HFRS) infected with Puumala virus (PUUV). The results showed suppression of the early immune response to PUUV i.e. genes coding for the synthesis of pattern recognition receptors, chemokines and their receptors, other cytokines, transcription factors as well as some signalling molecules. Functional analysis showed that down regulation of the expression of described set of genes modulates the inflammatory response by interfering with various cell-signalling pathways. For the first time, the biological importance of miRNA has been shown in the regulation of immune response during HFRS. Changes detected at the immune response level have been associated with disease severity but not with viral load in the blood. MAPK8, CCR5, IL-10, STAT1, STAT4, miR21 and miR19a could be potential predictors of HFRS pathogenesis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:832620
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-05-22 11:57:06