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master's thesis
Učinci fluorescentnih derivata indola na regulaciju staničnog ciklusa i preživljavanje stanica adenokarcinoma i fibroblasta pluća čovjeka
Zagreb: University of Zagreb, Faculty of Science, 2025. urn:nbn:hr:217:715724
Puzić, Jelena
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Puzić, J. (2025). Učinci fluorescentnih derivata indola na regulaciju staničnog ciklusa i preživljavanje stanica adenokarcinoma i fibroblasta pluća čovjeka (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:715724

Puzić, Jelena. "Učinci fluorescentnih derivata indola na regulaciju staničnog ciklusa i preživljavanje stanica adenokarcinoma i fibroblasta pluća čovjeka." Master's thesis, University of Zagreb, Faculty of Science, 2025. https://urn.nsk.hr/urn:nbn:hr:217:715724

Puzić, Jelena. "Učinci fluorescentnih derivata indola na regulaciju staničnog ciklusa i preživljavanje stanica adenokarcinoma i fibroblasta pluća čovjeka." Master's thesis, University of Zagreb, Faculty of Science, 2025. https://urn.nsk.hr/urn:nbn:hr:217:715724

Puzić, J. (2025). 'Učinci fluorescentnih derivata indola na regulaciju staničnog ciklusa i preživljavanje stanica adenokarcinoma i fibroblasta pluća čovjeka', Master's thesis, University of Zagreb, Faculty of Science, accessed 28 March 2025, https://urn.nsk.hr/urn:nbn:hr:217:715724

Puzić J. Učinci fluorescentnih derivata indola na regulaciju staničnog ciklusa i preživljavanje stanica adenokarcinoma i fibroblasta pluća čovjeka [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2025 [cited 2025 March 28] Available at: https://urn.nsk.hr/urn:nbn:hr:217:715724

J. Puzić, "Učinci fluorescentnih derivata indola na regulaciju staničnog ciklusa i preživljavanje stanica adenokarcinoma i fibroblasta pluća čovjeka", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2025. Available at: https://urn.nsk.hr/urn:nbn:hr:217:715724

Metadata
TitleUčinci fluorescentnih derivata indola na regulaciju staničnog ciklusa i preživljavanje stanica adenokarcinoma i fibroblasta pluća čovjeka
Title (english)Effects of fluorescent indole derivates on the cell cycle regulation and survival of human adenocarcinoma cells and lung fibroblast cells
AuthorJelena Puzić
MentorDragomira Majhen (mentor)
MentorNada Oršolić (komentor)
Committee memberNada Oršolić (predsjednik povjerenstva)
Committee memberBiljana Balen (član povjerenstva)
Committee memberNenad Malenica (član povjerenstva)
Committee memberTomislav Ivanković (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2025-01-29, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Fluorescentni indolni kationi zanimljivi su za terapeutsku upotrebu jer pokazuju jaku fluorescenciju i lokalizaciju u mitohondrijima. Modifikacijom indolnog prstena ovih spojeva, moguće je poboljšati njihova svojstva, poput fotostabilnosti i protutumorske aktivnosti, čime se povećava njihova učinkovitost u terapijama. Cilj ovog diplomskog rada bio je ispitati učinak novo sintetiziranih derivata indola na bazi vinil piridina na stanični ciklus, preživljenje, ekspresiju proteina uključenih u... More preživljavanje i regulaciju staničnog ciklusa te ispitati sposobnost lokalizacije spojeva u mitohondrijima stanica adenokarcinoma (A549) i fibroblasta pluća čovjeka (WI-38). Tretman stanica A549 i WI-38 spojevima FB404, FB411, FB414 i FB417 uzrokuje smanjenje preživljenja stanica. Smanjeno preživljenje stanica nakon tretmana spojevima nije uzrokovano aktivacijom apoptoze. Nadalje, spojevi FB404, FB406, FB411, FB414 i FB417 pokazuju značajnu lokalizaciju u mitohondrijima stanica A549 i WI-38. Tretman stanica A549 spojevima FB404, FB411, FB414 i FB417 uzrokuje zastoj u G0/G1 fazi staničnog ciklusa koji je vjerojatno posljedica povećane ekspresije proteina p21. Less
Abstract (english)
Fluorescent indole cations are of great interest for therapeutic use because they show strong fluorescence and localization in mitochondria. By modifying the indole ring of these compounds, it is possible to improve their properties, such as photostability and antitumor activity and therefore increase their effectiveness in therapies. The aim of this thesis was to examine the effect of newly synthesized indole derivatives based on vinyl pyridine on the cell cycle, survival, expression of... More proteins involved in survival and regulation of the cell cycle, and to examine the ability of the compounds to localize in the mitochondria of adenocarcinoma cells (A549) and human lung fibroblasts (WI-38). Treatment of A549 and WI-38 cells with compounds FB404, FB411, FB414 and FB417 causes a decrease in cell survival. Decreased cell survival after compound treatment was not caused by activation of apoptosis. Furthermore, the compounds FB404, FB406, FB411, FB414 and FB417 show significant localization in the mitochondria of A549 and WI-38 cells. Treatment of A549 cells with compounds FB404, FB411, FB414 and FB417 causes arrest in the G0/G1 phase of the cell cycle, which is probably due to increased expression of the p21 protein. Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:715724
Study programmeTitle: Experimental Biology-University graduate programme
Study programme type: university
Study level: graduate
Academic / professional title: sveučilišni/a magistar/magistra eksperimentalne biologije (sveučilišni/a magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2025-02-06 14:03:14
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