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doctoral thesis
Otkrivanje i važnost retrovirusne reverzne transkriptaze u staničnim kulturama
Zagreb: University of Zagreb, Faculty of Science, 2014. urn:nbn:hr:217:085344
Kutle, Leonida
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Kutle, L. (2014). Otkrivanje i važnost retrovirusne reverzne transkriptaze u staničnim kulturama (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:085344

Kutle, Leonida. "Otkrivanje i važnost retrovirusne reverzne transkriptaze u staničnim kulturama." Doctoral thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:085344

Kutle, Leonida. "Otkrivanje i važnost retrovirusne reverzne transkriptaze u staničnim kulturama." Doctoral thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:085344

Kutle, L. (2014). 'Otkrivanje i važnost retrovirusne reverzne transkriptaze u staničnim kulturama', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 11 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:085344

Kutle L. Otkrivanje i važnost retrovirusne reverzne transkriptaze u staničnim kulturama [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2014 [cited 2024 May 11] Available at: https://urn.nsk.hr/urn:nbn:hr:217:085344

L. Kutle, "Otkrivanje i važnost retrovirusne reverzne transkriptaze u staničnim kulturama", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:217:085344

Metadata
TitleOtkrivanje i važnost retrovirusne reverzne transkriptaze u staničnim kulturama
Title (english)Detection and significance of retroviral reverse transcriptase present in cell cultures
AuthorLeonida Kutle
MentorRenata Mažuran (mentor)
MBZ: 30040
Committee memberDijana Škorić (član povjerenstva)
Committee memberSnježana Židovec Lepej (član povjerenstva)
Committee memberDubravko Forčić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2014, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		576 - Cellular and subcellular biology. Cytology
Abstract
Enzim reverzna transkriptaza (RT) sastavni je dio svih retrovirusa i mjerenjem njene aktivnosti može se dokazati prisutnost retrovirusa u biološkim materijalima. Test eng. Product Enhanced Reverse Transcriptase (PERT) vrlo je osjetljiv test kojim se mogu detektirati vrlo niske koncentracije RT u uzorku. Kako bi test bio regulatorno prihvatljiv, potrebno je uvesti standard kao što je RT virusa ptičje mijeloblastoze. Svi testirani uzorci porijeklom s pilećeg supstrata sadrže aktivnost RT, odnosno retrovirusne čestice. Aktivnost RT otkrivena je i u uzorcima diploidnih i kontinuiranih staničnih kultura, te u mediju za rast i održavanje stanica s fetalnim goveđim serumom. Detektirana aktivnost RT ne potječe od infektivnih retrovirusnih čestica, budući da nema porasta aktivnosti RT u indikatorskim staničnim kulturama niti negativno utječe na miševe i zamorčad u testu opće neškodljivosti. Istražen je utjecaj različitih uvjeta proizvodnje cjepiva na pojavnost endogenih retrovirusa u proizvodnoj staničnoj kulturi. Kromatografijom na monolitnim nosačima moguće je koncentrirati i endogene retrovirusne čestice i molekule RT.
Abstract (english)
The enzyme reverse transcriptase (RT) is a part of all retroviruses, and the presence of retroviruses in biological samples can be detected by measuring RT's activity. Product Enhanced Reverse Transcriptase (PERT) test is a very sensitive assay for detection of low concentrations of RT in a sample. To make the assay regulatory acceptable, a standard like avian myeloblastosis virus RT should be included. All of the samples originated from chicken substrate show RT activity. RT activity is detected also in samples from diploid and continuous cell cultures, and in the cell culture media with foetal bovine serum. Detected activity originates from non-infectious retroviral particles, as no rise of the RT activity in indicator cell cultures is detected, nor it negatively affects mice and guinea-pigs in abnormal toxicity test. The effect of various operating conditions during the production of viral vaccines on RT activity was tested. Chromatography on monolithic columns enables concentration of endogenous retroviruses as well as RT molecules.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:085344
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-05-22 12:05:32