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master's thesis
Sinteza i karakterizacija peptidima funkcionaliziranih nanočestica i nanoklastera plemenitih metala i njihovo antibiotsko djelovanje
Zagreb: University of Zagreb, Faculty of Science, 2025. urn:nbn:hr:217:174441
Stefanović, Ines
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Stefanović, I. (2025). Sinteza i karakterizacija peptidima funkcionaliziranih nanočestica i nanoklastera plemenitih metala i njihovo antibiotsko djelovanje (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:174441

Stefanović, Ines. "Sinteza i karakterizacija peptidima funkcionaliziranih nanočestica i nanoklastera plemenitih metala i njihovo antibiotsko djelovanje." Master's thesis, University of Zagreb, Faculty of Science, 2025. https://urn.nsk.hr/urn:nbn:hr:217:174441

Stefanović, Ines. "Sinteza i karakterizacija peptidima funkcionaliziranih nanočestica i nanoklastera plemenitih metala i njihovo antibiotsko djelovanje." Master's thesis, University of Zagreb, Faculty of Science, 2025. https://urn.nsk.hr/urn:nbn:hr:217:174441

Stefanović, I. (2025). 'Sinteza i karakterizacija peptidima funkcionaliziranih nanočestica i nanoklastera plemenitih metala i njihovo antibiotsko djelovanje', Master's thesis, University of Zagreb, Faculty of Science, accessed 28 March 2025, https://urn.nsk.hr/urn:nbn:hr:217:174441

Stefanović I. Sinteza i karakterizacija peptidima funkcionaliziranih nanočestica i nanoklastera plemenitih metala i njihovo antibiotsko djelovanje [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2025 [cited 2025 March 28] Available at: https://urn.nsk.hr/urn:nbn:hr:217:174441

I. Stefanović, "Sinteza i karakterizacija peptidima funkcionaliziranih nanočestica i nanoklastera plemenitih metala i njihovo antibiotsko djelovanje", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2025. Available at: https://urn.nsk.hr/urn:nbn:hr:217:174441

Metadata
TitleSinteza i karakterizacija peptidima funkcionaliziranih nanočestica i nanoklastera plemenitih metala i njihovo antibiotsko djelovanje
Title (english)Synthesis and characterization of peptide-functionalized noble metal nanoparticles and nanoclusters and their antibiotic activity
AuthorInes Stefanović
MentorIvo Crnolatac (mentor)
Committee memberIva Juranović Cindrić (predsjednik povjerenstva)
Committee memberJana Pisk (član povjerenstva)
Committee memberĐani Škalamera (član povjerenstva)
Committee memberIvan Nemet (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2025-02-19, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Nanostrukture, primjerice nanočestice i nanoklasteri, mogu pokazivati citotoksičnu aktivnost ali i sposobnost da djeluju kao nosači određenih molekula (lijekovi, proteini...). U ovom se diplomskom radu provela sinteza nanočestica i nanoklastera srebra i zlata nakon čega su nanostrukture povezane s antimikrobnim peptidima. Antimikrobni peptidi su klasa kratkih peptida koji se mogu naći u prirodi, a imaju širok raspon inhibicijskih učinaka prema bakterijama i virusima. Koristili su se... More indolicidin, anoplin i PMAP-23. Cilj rada je bio da se njihovim povezivanjem ispita postojanje potencijalnog sinergijskog efekta koji dolazi od nanostruktura i antimikrobnih peptida zajedno. Povezivanjem nanostruktura s antimikrobnim peptidima dobile su se funkcionalizirane nanostrukture, čiji se antibiotski potencijal provjeravao stavljanjem na hranjivu podlogu s bakterijom Escherichia coli. Karakterizacija prekursora i produkata sinteze odvijala se različitim analitičkim metodama. Konjugat nanočestica-peptid se okarakterizirao fluorescencijskom spektrometrijom i dinamičkim raspršenjem svjetlosti. Izgled (oblik, struktura) i veličina nanostruktura se provjeravala elektronskom mikroskopijom i putem UV-Vis spektrofotometrije. Antimikrobni peptidi su karakterizirani cirkularnim dikroizmom i UV-Vis spektrofotometrijom. Less
Abstract (english)
Nanostructures, for example, nanoparticles and nanoclusters, can show cytotoxic activity and the ability to act as carriers of certain molecules (medicines, proteins...). In this thesis, nanoparticles and nanoclusters of silver and gold were synthesised, after which the nanostructures were conjugated with antimicrobial peptides. Antimicrobial peptides are a class of short peptides found in nature and have a wide range of inhibitory effects against bacteria and viruses. Indolicidin,... More anoplin and PMAP-23 were used. The goal was to examine the potential synergistic effect that comes from nanostructures and antimicrobial peptides together by connecting them. Functionalized nanostructures were obtained by linking nanostructures with antimicrobial peptides, whose antibiotic potential was determined by placing them on a nutrient medium with Escherichia coli. Characterization of precursors and synthesis products was carried out using various analytical methods. The nanoparticle-peptide conjugate was characterized by fluorescence spectrometry and dynamic light scattering. The appearance (shape, structure) and size of the nanostructures were determined by electron microscopy and UV-Vis spectrophotometry. Antimicrobial peptides were characterised by circular dichroism and UV-Vis spectrophotometry. Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:174441
Study programmeTitle: Graduate university studies in chemistry; specializations in: Graduate university studies in chemistry
Course: Graduate university studies in chemistry
Study programme type: university
Study level: graduate
Academic / professional title: sveučilišni/a magistar/magistra kemije (sveučilišni/a magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2025-02-24 13:45:53
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