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doctoral thesis
Strukturna analiza N-glikoma krvne plazme dugoživućeg golokrtičastoga štakora (Heterocephalus glaber Rüppell, 1842)
Zagreb: University of Zagreb, Faculty of Science, 2025. urn:nbn:hr:217:564336
Habazin, Siniša
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Habazin, S. (2025). Strukturna analiza N-glikoma krvne plazme dugoživućeg golokrtičastoga štakora (Heterocephalus glaber Rüppell, 1842) (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:564336

Habazin, Siniša. "Strukturna analiza N-glikoma krvne plazme dugoživućeg golokrtičastoga štakora (Heterocephalus glaber Rüppell, 1842)." Doctoral thesis, University of Zagreb, Faculty of Science, 2025. https://urn.nsk.hr/urn:nbn:hr:217:564336

Habazin, Siniša. "Strukturna analiza N-glikoma krvne plazme dugoživućeg golokrtičastoga štakora (Heterocephalus glaber Rüppell, 1842)." Doctoral thesis, University of Zagreb, Faculty of Science, 2025. https://urn.nsk.hr/urn:nbn:hr:217:564336

Habazin, S. (2025). 'Strukturna analiza N-glikoma krvne plazme dugoživućeg golokrtičastoga štakora (Heterocephalus glaber Rüppell, 1842)', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 28 March 2025, https://urn.nsk.hr/urn:nbn:hr:217:564336

Habazin S. Strukturna analiza N-glikoma krvne plazme dugoživućeg golokrtičastoga štakora (Heterocephalus glaber Rüppell, 1842) [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2025 [cited 2025 March 28] Available at: https://urn.nsk.hr/urn:nbn:hr:217:564336

S. Habazin, "Strukturna analiza N-glikoma krvne plazme dugoživućeg golokrtičastoga štakora (Heterocephalus glaber Rüppell, 1842)", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2025. Available at: https://urn.nsk.hr/urn:nbn:hr:217:564336

Metadata
TitleStrukturna analiza N-glikoma krvne plazme dugoživućeg golokrtičastoga štakora (Heterocephalus glaber Rüppell, 1842)
Title (english)Structural analysis of blood plasma N-glycome from a long-lived naked mole-rat (Heterocephalus glaber Rüppell, 1842)
AuthorSiniša Habazin
MentorMislav Novokmet (mentor)
Committee memberToma Keser (predsjednik povjerenstva)
Committee memberBiljana Balen (član povjerenstva)
Committee memberGöran Klobučar (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2025-02-24, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		57 - Biological sciences. Physical anthropology. Biodiversity
Abstract
Golokrtičasti štakor (GŠ) (Heterocephalus glaber Rüppell, 1842) je eusocijalni fosorijalni glodavac čiji je prirodni areal Istočna Afrika, a zbog životnog vijeka koji prelazi 30 godina je atraktivan modelni organizam u biologiji starenja. Molekularni mehanizmi otpornosti na kancerogenezu, neurodegeneraciju, hipoksiju i neke vrste boli povezani su sa održavanjem proteostaze u čemu sudjeluju i posttranslacijske modifikacije proteina. Dinamika i raznolikost glikozilacije proteina u GŠ do sada nije... More istraživana. U ovom je radu pomoću tekućinske kromatografije i spektrometrije masa provedena strukturna analiza N-glikoma krvne plazme GŠ. Određene su strukture i udjeli 160 različitih N-glikana među kojima se ističe strukturni motiv 4-O-acetilacije sijalinskih kiselina. Glikoproteomskom analizom otkriveno je da takvi sijaloglikani potječu s inhibitora proteaza u krvnoj plazmi te je pretpostavljen njihov zaštitni i stabilizirajući utjecaj na proteine. Less
Abstract (english)
The naked mole-rat (NMR) (Heterocephalus glaber Rüppell, 1842) is an eusocial fossorial rodent native to East Africa. Its exceptionally long lifespan of over 30 years makes it a popular non-conventional model organism in the biology of ageing and longevity. Molecular mechanisms underlying resistance to cancerogenesis, neurodegeneration, hypoxia and certain types of pain are related to the maintenance of proteostasis where key players are protein post-translational modifications. The dynamics... More and diversity of protein glycosylation in NMR are completely unexplored. In this study, a structural analysis of NMR blood plasma N-glycome was performed using liquid chromatography and mass spectrometry. A total od 160 different N-glycans were annotated and quantified. The core structural motif in the N-glycome of NMR was the presence of 4-O-acetylated sialic acids. Employing a glycoproteomic analysis, 4-Oacetylated sialo-N-glycans were pinpointed to plasma protease inhibitors and their protective and stabilizing effect on the proteins was inferred. Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:564336
ProjectNumber: KK.01.2.2.03.0006
Title: Centar kompetencija u molekularnoj dijagnostici
Acronym: CEKOM
Leader: Tomislav Krističević
Jurisdiction: eu
Funder: Europska unija
Funding stream: European Regional Development Fund
Study programmeTitle: Doctoral study
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
Type of resourceText
Extent173 str., 58 slika, 5 tablica, 236 literaturnih navoda
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2025-03-03 11:00:38
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