prikaz prve stranice dokumenta Utjecaj imunosnoga sustava na prisutnost cirkulirajućih melanomskih stanica
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doctoral thesis
Utjecaj imunosnoga sustava na prisutnost cirkulirajućih melanomskih stanica
Zagreb: University of Zagreb, Faculty of Science, 2013. urn:nbn:hr:217:306593
Marić Brozić, Jasmina
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Marić Brozić, J. (2013). Utjecaj imunosnoga sustava na prisutnost cirkulirajućih melanomskih stanica (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:306593

Marić Brozić, Jasmina. "Utjecaj imunosnoga sustava na prisutnost cirkulirajućih melanomskih stanica." Doctoral thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:306593

Marić Brozić, Jasmina. "Utjecaj imunosnoga sustava na prisutnost cirkulirajućih melanomskih stanica." Doctoral thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:306593

Marić Brozić, J. (2013). 'Utjecaj imunosnoga sustava na prisutnost cirkulirajućih melanomskih stanica', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 30 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:306593

Marić Brozić J. Utjecaj imunosnoga sustava na prisutnost cirkulirajućih melanomskih stanica [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2013 [cited 2024 December 30] Available at: https://urn.nsk.hr/urn:nbn:hr:217:306593

J. Marić Brozić, "Utjecaj imunosnoga sustava na prisutnost cirkulirajućih melanomskih stanica", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:217:306593

Metadata
TitleUtjecaj imunosnoga sustava na prisutnost cirkulirajućih melanomskih stanica
Title (english)Impact of immune system on presence of circulating melanoma cells
AuthorJasmina Marić Brozić
MentorIvan Šamija (mentor)
MBZ: 266224
Committee memberZvonko Kusić (član povjerenstva)
MBZ: 119493
Committee memberNada Oršolić (član povjerenstva)
Committee memberIvan Šamija (član povjerenstva)
MBZ: 266224
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2013, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
Metoda RT-PCR specifična za tirozinazu je visoko specifična i osjetljiva metoda otkrivanja cirkulirajućih melanomskih stanica (CMS). Iako CMS mogu biti cilj specifične imunološke reakcije protiv tumora, povezanost između imunokompetencije i prisutnosti CMS nije dosad istraživana. Cilj ovog rada je istražiti postoji li povezanost između prisutnosti CMS i različitih parametara imunološkog statusa. Analizirani su uzorci krvi 131 bolesnika s melanomom. Prisutnost melanomskih stanica u uzorcima krvi određena je metodom RT-PCR specifičnom za tirozinazu. Udio različitih skupina limfocita u uzorcima krvi kao parametar imunološkog statusa određen je protočnom citometrijom. Aktivnost stanica NK i fagocitne funkcije granulocita i monocita analizirani su kao funkcionalni parametri imunološkog statusa. Pokazana je visoka specifičnost i nizak prag osjetljivosti tirozinaze kao biljega za određivanje CMS. Bolesnici s pozitivnom vrijednošću tirozinaze imali su značajno kraće preživljenje čime sam potvrdila prognostičku vrijednost tirozinaze. Nije nađena povezanost između prisutnosti CMS i istraživanih parametara imunološkog statusa.
Abstract (english)
Tyrosinase-specific RT-PCR is highly sensitive and specific method for detection of circulating melanoma cells (CMC). Although CMC might be target of tumor-specific immune reaction, correlation between immunocompetence and presence of CMC hasn’t been studied so far. The aim of this study was to investigate if there is an association between the presence of CMC and different parameters of immune status. Blood samples from 131 patients with melanoma were analyzed. The presence of CMC was analyzed by tyrosinase-specific RT-PCR. Proportions of lymphocyte subsets in blood samples as a parameter of immune status were determined by flow-cytometry. Activity of NK cells and phagocytic functions of granulocytes and monocytes were analyzed as functional parameters of immune status. High specificity and low threshold for detection were shown for tyrosinase as a marker for CMC detection by RT-PCR. Patients positive for tyrosinase had significantly shorter survival, which confirms its prognostic value. No association between the presence of CMC and parameters of immune status was found.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:306593
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-24 08:56:50