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master's thesis
Kvantitativna analiza telomera u ljudskim stanicama MJ-90hTERT
Zagreb: University of Zagreb, Faculty of Science, 2017. urn:nbn:hr:217:050288
Stančić, Vedran
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Stančić, V. (2017). Kvantitativna analiza telomera u ljudskim stanicama MJ-90hTERT (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:050288

Stančić, Vedran. "Kvantitativna analiza telomera u ljudskim stanicama MJ-90hTERT." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:050288

Stančić, Vedran. "Kvantitativna analiza telomera u ljudskim stanicama MJ-90hTERT." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:050288

Stančić, V. (2017). 'Kvantitativna analiza telomera u ljudskim stanicama MJ-90hTERT', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:050288

Stančić V. Kvantitativna analiza telomera u ljudskim stanicama MJ-90hTERT [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2017 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:050288

V. Stančić, "Kvantitativna analiza telomera u ljudskim stanicama MJ-90hTERT", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:217:050288

Metadata
TitleKvantitativna analiza telomera u ljudskim stanicama MJ-90hTERT
Title (english)A quantitative analysis of the dynamic of telomeres in human MJ-90hTERT cells
AuthorVedran Stančić
MentorIvica Rubelj (mentor)
MentorVišnja Besendorfer (mentor)
Committee memberVišnja Besendorfer (predsjednik povjerenstva)
Committee memberRenata Matoničkin Kepčija (član povjerenstva)
Committee memberBožena Mitić (član povjerenstva)
Committee memberNataša Bauer (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2017-04-24, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Tema rada je kvantitativna analiza telomera u ljudskim stanicama MJ-90hTERT. To su fibroblasti imortalizirani telomerazom koja im omogućava produžavanje telomera, ali koji imaju fenotip i kontrolu rasta jednaku normalnim fibroblastima. Telomeraza produžuje pojedine telomere u ovisnosti o njihovoj konformaciji, odnosno duljini. Nakon označavanja telomera metodom hibridizacije in situ Q-PNA-FISH napravljena je kvantitativna analiza. Ovom se metodom može analizirati relativna duljina svake pojedine telomere i telomernog para na metafaznim kromosomima. Napravljena je usporedba duljina telomera sestrinskih kromatida fibroblasta koji su prošli 45, 60 i 75 udvostručenja populacije (PD) nakon unošenja telomeraze. Analiza promjena u duljini telomera pokazala je ujednačivanje njihove duljine razmjerno broju ciklusa kroz koji je stanična populacija prošla. Također je statistički pokazana pouzdanost metode Q-PNA-FISH u analizi telomera.
Abstract (english)
The topic of this master thesis is a quantitative analysis of the dynamic of telomeres in human MJ-90 hTERT cells. This fibroblast cell line is immortalized by telomerase which enables telomere lenghtening, but the cells retain the same phenotype and growth control as in normal fibroblasts. Telomerase lenghtens telomeres in dependence on their conformation and/or length. Telomeres in these cells are quantitatively analyzed with software after the in situ hybridization with the Q-PNA-FISH method. This method enables analysis of each telomere and telomere pair on metaphase chromosomes. The length of sister chromatid telomeres were compared in fibroblasts which grew for 45, 60 and 75 population doublings in culture, after immortalization by telomerase. The analysis of changes in telomere length showed tendency of equalization in telomere length during the growth in the culture. Besides that, the reliability of Q-PNA-FISH in telomere analysis was statistically demonstrated.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:050288
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-05-26 07:59:02