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master's thesis
Razvoj metode HPLC za određivanje serotonina, dopamina i noradrenalina u tkivu mozga štakora
Zagreb: University of Zagreb, Faculty of Science, 2017. urn:nbn:hr:217:628443
Peršić, Dora
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Peršić, D. (2017). Razvoj metode HPLC za određivanje serotonina, dopamina i noradrenalina u tkivu mozga štakora (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:628443

Peršić, Dora. "Razvoj metode HPLC za određivanje serotonina, dopamina i noradrenalina u tkivu mozga štakora." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:628443

Peršić, Dora. "Razvoj metode HPLC za određivanje serotonina, dopamina i noradrenalina u tkivu mozga štakora." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:628443

Peršić, D. (2017). 'Razvoj metode HPLC za određivanje serotonina, dopamina i noradrenalina u tkivu mozga štakora', Master's thesis, University of Zagreb, Faculty of Science, accessed 04 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:628443

Peršić D. Razvoj metode HPLC za određivanje serotonina, dopamina i noradrenalina u tkivu mozga štakora [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2017 [cited 2024 November 04] Available at: https://urn.nsk.hr/urn:nbn:hr:217:628443

D. Peršić, "Razvoj metode HPLC za određivanje serotonina, dopamina i noradrenalina u tkivu mozga štakora", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:217:628443

Metadata
TitleRazvoj metode HPLC za određivanje serotonina, dopamina i noradrenalina u tkivu mozga štakora
Title (english)Development of a HPLC method for serotonin, dopamine and noradrenaline determination in rat brain tissue
AuthorDora Peršić
MentorSofia Ana Blažević (mentor)
Committee memberSofia Ana Blažević (predsjednik povjerenstva)
Committee memberMirta Tkalec (član povjerenstva)
Committee memberBiljana Balen (član povjerenstva)
Committee memberIvana Šola (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2017-05-31, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Serotonin, dopamin i noradrenalin neurotransmiteri su s mnogim funkcijama u mozgu. Promjene u njihovom metabolizmu povezane su s neurorazvojnim poremećajima, što određivanje njihovih razina čini ključnim za razumijevanje tih poremećaja. Analitička metoda koja se često koristi u tu svrhu je tekućinska kromatografija visoke djelotvornosti (engl. high-performance liquid chromatography, HPLC). Za analizu traženih spojeva potrebno je razviti metodu, a razvijenu metodu zatim treba validirati. U ovom diplomskom radu, razvila sam brzu metodu HPLC s UV detekcijom za određivanje koncentracija serotonina, dopamina i noradrenalina u tkivu mozga štakora. Razvijenu metodu validirala sam odredivši njenu selektivnost, linearnost i ponovljivost za svaki analit. Rezultati validacije pokazali su dobru selektivnost za sve analite. Linearnost i ponovljivost bile su dobre za serotonin, dok za dopamin i noradrenalin nisu bile u potpunosti zadovoljavajuće, vjerojatno uslijed njihove oksidativne degradacije. U usporedbi koncentracija određenih razvijenom metodom s onima dobivenim komercijalnom metodom ELISA nisam ustanovila korelaciju, stoga rezultati upućuju na potrebu za pronalaskom boljeg načina pripreme i pohrane uzoraka.
Abstract (english)
Serotonin, dopamine and noradrenaline are neurotransmitters with many functions in the brain. Changes in their metabolism have been implicated in various neurodevelopmental disorders, making determination of their levels necessary for a better understanding of those disorders. An analytical method often used for this purpose is high-performance liquid chromatography (HPLC). In this study, I have developed a rapid HPLC method with UV detection for serotonin, dopamine and noradrenaline determination in rat brain tissue. I have validated the developed method by analyzing its selectivity, linearity and repeatability. The method showed good selectivity for all three neurotransmitters. Linearity and repeatability were good for serotonin, while they were not completely satisfactory for dopamine and noradrenaline, pressumably due to their oxidative degradation. Comparison of serotonin, dopamine and noradrenaline levels determined by the developed method with those obtained by a commercially available ELISA method did not show any correlation, indicating a need for further improvement of sample preparation and storage.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:628443
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2019-05-31
Terms of useLicence In copyright icon
Created on2017-07-14 09:21:17