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master's thesis
Promjene u ultrastrukturi, proteomu i fotosintetskoj učinkovitosti klijanaca duhana (Nicotiana tabacum L.) pod utjecajem nanočestica i iona srebra
Zagreb: University of Zagreb, Faculty of Science, 2017. urn:nbn:hr:217:287734
Banić, Elizabeta
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Banić, E. (2017). Promjene u ultrastrukturi, proteomu i fotosintetskoj učinkovitosti klijanaca duhana (Nicotiana tabacum L.) pod utjecajem nanočestica i iona srebra (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:287734

Banić, Elizabeta. "Promjene u ultrastrukturi, proteomu i fotosintetskoj učinkovitosti klijanaca duhana (Nicotiana tabacum L.) pod utjecajem nanočestica i iona srebra." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:287734

Banić, Elizabeta. "Promjene u ultrastrukturi, proteomu i fotosintetskoj učinkovitosti klijanaca duhana (Nicotiana tabacum L.) pod utjecajem nanočestica i iona srebra." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:287734

Banić, E. (2017). 'Promjene u ultrastrukturi, proteomu i fotosintetskoj učinkovitosti klijanaca duhana (Nicotiana tabacum L.) pod utjecajem nanočestica i iona srebra', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:287734

Banić E. Promjene u ultrastrukturi, proteomu i fotosintetskoj učinkovitosti klijanaca duhana (Nicotiana tabacum L.) pod utjecajem nanočestica i iona srebra [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2017 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:287734

E. Banić, "Promjene u ultrastrukturi, proteomu i fotosintetskoj učinkovitosti klijanaca duhana (Nicotiana tabacum L.) pod utjecajem nanočestica i iona srebra", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:217:287734

Metadata
TitlePromjene u ultrastrukturi, proteomu i fotosintetskoj učinkovitosti klijanaca duhana (Nicotiana tabacum L.) pod utjecajem nanočestica i iona srebra
Title (english)Changes in ultrastructure, proteome and photosynthetic fficiency of tobacco seedlings (Nicotiana tabacum L.) exposed to silver nanoparticles and ions
AuthorElizabeta Banić
MentorPetra Peharec Štefanić (mentor)
Committee memberPetra Peharec Štefanić (predsjednik povjerenstva)
Committee memberMirta Tkalec (član povjerenstva)
Committee memberMartina Šeruga Musić (član povjerenstva)
Committee memberNenad Malenica (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2017-06-19, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Nanočestice srebra (AgNP) se zbog antimikrobnih svojstava koriste u širokom spektru nanotehnoloških proizvoda, koji se, između ostaloga, koriste u medicini, prehrambenoj tehnologiji i proljoprivredi. Toksičnost AgNP dokazana je na brojnim organizmima, a ovisi o veličini, obliku i površinskom naboju nanočestica, tipu molekula-omotača i otpuštanju iona srebra. Duhan (Nicotiana tabucum L.) je ekonomski važna kultura i u molekularnoj biologiji uvriježen modelni organizam. U ovom diplomskom radu istražila sam utjecaj AgNP istog oblika, veličine i koncentracije, a stabiliziranih različitim omotačima (bPEI, citrat ili PEG), na klijance duhana. Sjemenke duhana isklijavala sam u tekućem mediju s dodatkom različitih AgNP ili srebrovog nitrata u koncentraciji 100 μM, a u slučaju kontrole bez dodatka srebra. Klijance sam koristila za istraživanje desetog dana tretmana. Tretmani su inhibirali rast korijena, narušili strukturu meristema korijena i uzrokovali promjene u ultrastrukturi kloroplasta. Sadržaj fotosintetskih pigmenata i parametri fluorescencije klorofila također su bili promijenjeni. Proteom klijanaca duhana pokazao je povećanu ekspresiju proteina prvenstveno vezanih uz fotosintezu i odgovor na stres. Nekoliko proteina pokazalo je različit odgovor na nanočestice i ione srebra. AgNP različitih omotača utjecale su na klijance duhana na različit način, no pokazale su se toksičnijima od iona srebra iste koncentracije.
Abstract (english)
Silver nanoparticles (AgNPs) are used for their antimicrobial properties in a wide variety of nanotechnology products used in many fields, including medicine, food technology, and agriculture. The toxicity of AgNPs was proven on many organisms and depends on their size, shape, surface charge, type of coating and release of silver ions. Tobacco (Nicotiana tabucum L.) is an important cash crop and an established model organism in molecular biology. In this thesis, I investigated the influence of AgNPs of the same shape, size, and concentration and stabilised with different coatings (bPEI, citrate or PEG) on tobacco seedlings. Tobacco seeds were germinated in liquid medium containing different AgNPs or silver nitrate in 100 μM concentration. In the case of control, no silver was added. The seedlings were used for research on the tenth day of the treatment. The treatments inhibited root growth, disrupted root meristem structure and caused changes in chloroplast ultrastructure. Photosynthetic pigments' content and chlorophyll fluorescence parameters also changed. Tobacco seedling proteome showed an increased expression of proteins, primarily related to photosynthesis and stress response. A few proteins differed in response to AgNP and silver ion treatment. Differently coated AgNPs affected tobacco seedlings in a different manner and showed to be more toxic than silver ions of equal concentration.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:287734
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2018-09-19
Terms of useLicence In copyright icon
Created on2017-07-14 09:31:03