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master's thesis
Optogenetička metoda za brzo i reverzibilno uklanjanje proteina iz diobenog vretena
Zagreb: University of Zagreb, Faculty of Science, 2017. urn:nbn:hr:217:814147
Milas, Ana
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Milas, A. (2017). Optogenetička metoda za brzo i reverzibilno uklanjanje proteina iz diobenog vretena (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:814147

Milas, Ana. "Optogenetička metoda za brzo i reverzibilno uklanjanje proteina iz diobenog vretena." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:814147

Milas, Ana. "Optogenetička metoda za brzo i reverzibilno uklanjanje proteina iz diobenog vretena." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:814147

Milas, A. (2017). 'Optogenetička metoda za brzo i reverzibilno uklanjanje proteina iz diobenog vretena', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:814147

Milas A. Optogenetička metoda za brzo i reverzibilno uklanjanje proteina iz diobenog vretena [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2017 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:814147

A. Milas, "Optogenetička metoda za brzo i reverzibilno uklanjanje proteina iz diobenog vretena", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:217:814147

Metadata
TitleOptogenetička metoda za brzo i reverzibilno uklanjanje proteina iz diobenog vretena
Title (english)Optogenetic method for rapid and reversible removal of proteins from the mitotic spindle
AuthorAna Milas
MentorIva Tolić (mentor)
Committee memberJasmina Rokov Plavec (predsjednik povjerenstva)
Committee memberBranimir Bertoša (član povjerenstva)
Committee memberIva Juranović Cindrić (član povjerenstva)
Committee memberDavor Kovačević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2017-07-11, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Na početku mitoze mikrotubuli i pripadajući proteini formiraju diobeno vreteno, strukturu koja omogućuje točnu raspodjelu kromosoma između dvije stanice kćeri. U ovom radu razvijena je optogenetička metoda koja omogućuje brzu i reverzibilnu translokaciju proteina iz diobenog vretena na mitohondrije korištenjem plavog svjetla. Metoda je primijenjena za proučavanje proteina TACC3 i PRC1 koji sudjeluju u formiranju paralelnih, odnosno antiparalelnih svežnjeva mikrotubula. Translokacija oba proteina događala se na vremenskim skalama od nekoliko minuta. Mijenjanjem koncentracije proteina PRC1 na diobenom vretenu bilo je moguće inducirati raspadanje i ponovno formiranje antiparalelnih svežnjeva mikrotubula što je rezultiralo promjenom oblika diobenog vretena. Dakle, ova metoda predstavlja novi moćni alat za proučavanje proteina diobenog vretena kontrolom njihove lokalizacije u pojedinim fazama mitoze.
Abstract (english)
At the onset of mitosis microtubules and microtubule associated proteins form a spindle, which is responsible for proper segregation of chromosomes between two daughter cells. In this thesis, I have developed optogenetic method for rapid and reversible translocation of proteins from the mitotic spindle to mitochondria using blue light. This method was used for studying TACC3 and PRC1, proteins which are required for formation of parallel and antiparallel microtubule bundles, respectively. Translocation of both proteins occurred at time scales of several minutes. By changing concentration of PRC1 in the mitotic spindle, I was able to induce disassembly and formation of antiparallel microtubule bundles, which resulted in changes in the spindle shape. Thus, this method represents powerful new tool for studying spindle proteins by controlling their localization at specific stages of mitosis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:814147
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2019-07-11
Terms of useLicence In copyright icon
Created on2017-07-18 11:44:24