prikaz prve stranice dokumenta Struktura genoma, kemijski sastav i biološka aktivnost triploidnog luka Allium × cornutum Clementi ex Visiani
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doctoral thesis
Struktura genoma, kemijski sastav i biološka aktivnost triploidnog luka Allium × cornutum Clementi ex Visiani
Zagreb: University of Zagreb, Faculty of Science, 2017. urn:nbn:hr:217:480919
Fredotović, Željana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Fredotović, Ž. (2017). Struktura genoma, kemijski sastav i biološka aktivnost triploidnog luka Allium × cornutum Clementi ex Visiani (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:480919

Fredotović, Željana. "Struktura genoma, kemijski sastav i biološka aktivnost triploidnog luka Allium × cornutum Clementi ex Visiani." Doctoral thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:480919

Fredotović, Željana. "Struktura genoma, kemijski sastav i biološka aktivnost triploidnog luka Allium × cornutum Clementi ex Visiani." Doctoral thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:480919

Fredotović, Ž. (2017). 'Struktura genoma, kemijski sastav i biološka aktivnost triploidnog luka Allium × cornutum Clementi ex Visiani', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:480919

Fredotović Ž. Struktura genoma, kemijski sastav i biološka aktivnost triploidnog luka Allium × cornutum Clementi ex Visiani [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2017 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:480919

Ž. Fredotović, "Struktura genoma, kemijski sastav i biološka aktivnost triploidnog luka Allium × cornutum Clementi ex Visiani", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:217:480919

Metadata
TitleStruktura genoma, kemijski sastav i biološka aktivnost triploidnog luka Allium × cornutum Clementi ex Visiani
Title (english)Genome structure, chemical composition and biological activity of triploid onion Allium × cornutum Clementi ex Visiani
AuthorŽeljana Fredotović
MentorJasna Puizina (mentor)
MBZ: 171072
Committee memberMirjana Pavlica (član povjerenstva)
Committee memberKroata Hazler-Pilepić (član povjerenstva)
MBZ: 201953
Committee memberVlatka Zoldoš (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2017, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		57 - Biological sciences. Physical anthropology. Biodiversity
Abstract
Allium × cornutum Clementi ex Visiani jedinstvena je forma luka, koja se pod nazivom 'Ljutika' tradicionalno uzgaja i koristi u ljudskoj prehrani u obalnoj i otočnoj Hrvatskoj. Otporna je na sušu i hladnoću, te prilagođena oskudnom, kamenitom tlu. Ovim radom željela se detaljnije istražiti struktura genoma te vrste, rasvijetliti nutritivni sastav te provjeriti njezinu biološku aktivnost. Provedene molekularno-filogenetske analize ribosomskih gena, 18S-5.8S-26S rDNA i 5S rDNA, otkrile su da je A. × cornutum (2n = 3x = 24) jedinstveni hibrid triju vrsta lukova: crvenog luka, Allium cepa L. i dva azijska luka, Allium pskemense B. Fedtsch i Allium roylei Stearn. Po jedan haploidni genom (x = 8) svake od triju roditeljskih vrsta identificiran je i vizualiziran na mitotskim i mejotičkim kromosomima A. × cornutum metodom fluorescentne hibridizacije in situ. Analize kloroplastnih i mitohondrijskih gena, pokazuju da A. × cornutum posjeduje jedinstveni tip sterilne citoplazme CMS-S, važne u oplemenjivanju lukova. Kemijske analize metanolnih ekstrakta A. × cornutum i A. cepa izvršene metodom HPLC-a, ukazuju na povećan sadržaj flavonoida (flavonola i antocijanina) u A. × cornutum, što može biti objašnjenje i nešto boljeg bioaktivnog profila triploida u odnosu na A. cepa. Obje vrste pokazuju značajnu antioksidativnu, antigenotoksičnu i antiproliferacijsku aktivnost, što ih potvrđuje kao važan izvor korisnih fitonutrijenata koji mogu doprinijeti zaštiti i očuvanju ljudskog zdravlja.
Abstract (english)
Allium × cornutum Clementi ex Visiani is unique onion type, traditionally cultivated in South and coastal Croatia under the name 'Ljutika' and used in human diet. It is resistant to cold weather conditiones and drought, and adapted to growth on poor stony ground. This paper attempts to explore genome structure, shed light on the nutritional composition and check biological activity of this species. Molecular-cytogenetic and phylogenetic analysis of two ribosomal genes, 18S-5.8S-26S rDNA and 5S rDNA revealed that 'Ljutika' is a unique hybrid of three onion species: common onion, Allium cepa L., and two Asian onions, Allium pskemense B. Fedtsch and Allium roylei Stearn. One haploid genome (x = 8) of each of the three parent species was identified and visualized on mitotic and meiotic chromosomes of A. × cornutum by fluorescent in situ hybridization. Chloroplast and mitochondrial gene analysis showed that 'Ljutika' possesses a sterile CMS-S type of cytoplasm, important for onion breeding. Phytochemical analysis determined by HPLC showed that methanolic extracts of A. × cornutum and A. cepa showed slightly higher content of flavonoids (flavonols and anthocyanins) in A. × cornutum, which may be cause of its higher bioactive profile in compare to A. cepa. Both species showed a significant antioxidant, antigenotoxic and antiproliferative activity which makes them an important sources of phytonutrients that can contribute to the protection and preservation of human health.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:480919
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-07-20 09:16:31