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doctoral thesis
Imunostimulatorni i imunomodulatorni učinak derivata fenantridina
Zagreb: University of Zagreb, Faculty of Science, 2017. urn:nbn:hr:217:790463
Knežević Teofilović, Natalija
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Knežević Teofilović, N. (2017). Imunostimulatorni i imunomodulatorni učinak derivata fenantridina (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:790463

Knežević Teofilović, Natalija. "Imunostimulatorni i imunomodulatorni učinak derivata fenantridina." Doctoral thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:790463

Knežević Teofilović, Natalija. "Imunostimulatorni i imunomodulatorni učinak derivata fenantridina." Doctoral thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:790463

Knežević Teofilović, N. (2017). 'Imunostimulatorni i imunomodulatorni učinak derivata fenantridina', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:790463

Knežević Teofilović N. Imunostimulatorni i imunomodulatorni učinak derivata fenantridina [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2017 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:790463

N. Knežević Teofilović, "Imunostimulatorni i imunomodulatorni učinak derivata fenantridina", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:217:790463

Metadata
TitleImunostimulatorni i imunomodulatorni učinak derivata fenantridina
Title (english)Immunnostimulatory and immunomodulatory potential of different phenanthridine derivatives
AuthorNatalija Knežević Teofilović
MentorNada Oršolić (mentor)
Committee memberJasmina Rokov Plavec (predsjednik povjerenstva)
Committee memberNada Oršolić (član povjerenstva)
Committee memberMarijana Radić Stojković (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2017-07-10, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Universal decimal classification
(UDC)		54 - Chemistry. Crystallography. Mineralogy
Abstract
Toll-like receptori (TLR) sudjeluju u pokretanju prirođene i stečene imunosti. Fenantridini su heterociklički aromatski ugljikovodici čija struktura je slična strukturi nukleotidnih analoga koji su sintetski ligandi za TLR7 i TLR8. Poznati sintetski agonist TLR3 je analog dvostruke RNA (poli(I:C)), dok su TLR9 agonisti oligonukleotidi koji sadrže nemetilirane CpG dinukleotide (CpG-ODN). Istraživanja su pokazala da fenantridini imaju svojstvo ugradnje u strukture dvolančane DNA; potencijalno imunomodulatorno svojstvo. U ovom istraživanju smo testirali 17 fenantridinskih derivata, pri čemu je spoj M-199 pokazao imunostimulacijski učinak. U ispitivanjima je pokazano da M-199 potiče izlučivanje IL-6, IL-8 i TNF-α, dok ne utječe na promjenu sadržaja IFN-α i IL-1β. Također smo pokazali da spoj M-199 ne djeluje preko TLR7 i TLR8. Tijekom ispitivanja međudjelovanja sa ligandima za TLR9 i TLR3, pokazano je da spoj M-199 ne utječe na stimulaciju TLR9 koja je posljedica vezanja liganda ODN2006, dok suprimira stimulaciju posredovanu ligandom ODN2216. M-199, također suprimira djelovanje poli(I:C).
Abstract (english)
Toll-like receptors (TLR) are responsible for activation and regulation of innate and adaptive immune response. Synthetic ligands activating TLR7 and TLR8 are nucleoside analogues resembling phenanthridine structure. Phenanthridines are heterocyclic aromatic hydrocarbons. Synthetic ligands for TLR3 are double-stranded RNA analogues (poly(I:C)) and ligands for TLR9 are oligonucleotides containing unmethylated CpG dinucleotide (CpG-ODN). Phenanthridine derivatives also intercalate in double-stranded DNA structure; having possible immunomodulatory potential. In line with afore-mentioned we have tested 17 different phenanthridine derivatives and concluded that molecule M-199 shows immunostimulatory potential. In further analysis it was shown that M-199 stimulates synthesis of IL-6, IL-8 and TNF-α, while it has no effect on the levels of IFN-α and IL-1β. It was also shown that M-199 does not signal throught TLR7 or TLR8. The results of the interaction study indicated that M-199 does not interfere with ODN2009 while it interferes with ODN2216 and poli(I:C) showing suppression of stimulation.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:790463
Study programmeTitle: Doctoral study in chemistry
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija (doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija)
Type of resourceText
Extent122 str. ; 30 cm
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-07-26 11:40:14