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master's thesis
Utjecaj proteina Spartan na ekspresiju gena uključenih u popravak DNA
Zagreb: University of Zagreb, Faculty of Science, 2017. urn:nbn:hr:217:268210
Ančić, Anja
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Ančić, A. (2017). Utjecaj proteina Spartan na ekspresiju gena uključenih u popravak DNA (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:268210

Ančić, Anja. "Utjecaj proteina Spartan na ekspresiju gena uključenih u popravak DNA." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:268210

Ančić, Anja. "Utjecaj proteina Spartan na ekspresiju gena uključenih u popravak DNA." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:268210

Ančić, A. (2017). 'Utjecaj proteina Spartan na ekspresiju gena uključenih u popravak DNA', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:268210

Ančić A. Utjecaj proteina Spartan na ekspresiju gena uključenih u popravak DNA [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2017 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:268210

A. Ančić, "Utjecaj proteina Spartan na ekspresiju gena uključenih u popravak DNA", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:217:268210

Metadata
TitleUtjecaj proteina Spartan na ekspresiju gena uključenih u popravak DNA
Title (english)Influence of Spartan protein on DNA repair genes expression
AuthorAnja Ančić
MentorIvana Marinović Terzić (mentor)
MentorIvana Ivančić Baće (mentor)
Committee memberIvana Ivančić Baće (predsjednik povjerenstva)
Committee memberGordana Lacković-Venturin (član povjerenstva)
Committee memberIvana Šola (član povjerenstva)
Committee memberNataša Bauer (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2017-08-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Popravak DNA oštećenja je nužan za održavanje stabilnosti genoma i visoke vjernosti replikacije genoma. Homologna rekombinacija, nehomologno sparivanje krajeva i popravak krivo sparenih baza su glavni mehanizmi popravka oštećenja DNA koji doprinose očuvanju genoma. Zbog stalne izloženosti ljudskog genoma štetnim tvarima iz okoliša i nusproduktima staničnog metabolizma, važno je pravilno djelovanje navedenih mehanizama. Ukoliko oštećenja ostanu nepopravljena mogu inducirati apoptozu, kromosomske aberacije, uzrokovati anomalije u razvoju, maligne bolesti i preuranjeno starenje (progeriju). Spartan je protein koji sudjeluje u održavanju stabilnosti genoma i pomoću metaloproteazne domene uklanja proteine kovalentno vezane za DNA, jednu od najčešćih tipova DNA lezija. Mutacije u genu SPRTN kod ljudi uzrokuju preuranjeno starenje i razvoj hepatocelularnog karcinoma u adolescentnoj dobi. Oštećenja u obliku kovalentno vezanih proteina na DNA se nakupljaju i u drugim vrstama stanica, ali specifično jetrene stanice razvijaju karcinom, što znači da ostale vrste stanica kompenziraju nedostatak proteina Spartan. U ovom radu istraživano je utječe li smanjenje količine ili poremećaj funkcije proteina Spartan na ekspresiju gena čiji proteini sudjeluju u različitim putevima popravka oštećenja DNA. Mjerenjem ekspresije gena lančanom reakcijom polimerazom u stvarnom vremenu uočili smo da postoji ovisnost ekspresije gena uključenih u popravak DNA o funkciji i ekspresiji gena SPRTN.
Abstract (english)
DNA repair is crucial for maintaining genome stability and high fidelity replication. Homologous recombination, non-homologous end joining and mismatch repair are the main repair mechanisms of DNA damage that contribute to the maintenance of the genome. Due to constant exposure of human genome to harmful agents from the environment and byproducts of cell metabolism, the proper functioning of these mechanisms is important. If the damage remains unrepaired, it can induce apoptosis, chromosomal aberrations, developmental anomalies, malignancy and premature aging. Spartan is protein involved in maintaining the genome stability and with metalloprotease domain removes crosslinked proteins from DNA, one of the most common types of DNA lesions. Mutations in the SPRTN gene in humans cause premature aging and hepatocellular carcinoma. Crosslinked proteins also accumulate on DNA in other cell types, but specifically liver cells develop cancer, which suggests that other types of cells compensate for the lack of Spartan protein. This paper investigated effects of the amount reduction or function disorder of the Spartan protein on the expression of genes whose proteins participate in various repair pathways of DNA damage. Measuring the expression of genes with quantitative realtime polymerase chain reaction, we have observed that correlation between expression of genes involved in DNA repair and SPRTN exist.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:268210
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2018-12-31
Terms of useLicence In copyright icon
Created on2017-09-07 09:48:06