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master's thesis
Vijabilnost stanične linije HEK 293 u prisustvu sintetiziranih nanočestica zlata
Zagreb: University of Zagreb, Faculty of Science, 2017. urn:nbn:hr:217:174730
Jančec, Marta
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Jančec, M. (2017). Vijabilnost stanične linije HEK 293 u prisustvu sintetiziranih nanočestica zlata (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:174730

Jančec, Marta. "Vijabilnost stanične linije HEK 293 u prisustvu sintetiziranih nanočestica zlata." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:174730

Jančec, Marta. "Vijabilnost stanične linije HEK 293 u prisustvu sintetiziranih nanočestica zlata." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:174730

Jančec, M. (2017). 'Vijabilnost stanične linije HEK 293 u prisustvu sintetiziranih nanočestica zlata', Master's thesis, University of Zagreb, Faculty of Science, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:174730

Jančec M. Vijabilnost stanične linije HEK 293 u prisustvu sintetiziranih nanočestica zlata [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2017 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:217:174730

M. Jančec, "Vijabilnost stanične linije HEK 293 u prisustvu sintetiziranih nanočestica zlata", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:217:174730

Metadata
TitleVijabilnost stanične linije HEK 293 u prisustvu sintetiziranih nanočestica zlata
Title (english)Viability of cell line HEK 293 in the presence of synthesized gold nanoparticles
AuthorMarta Jančec
MentorMarijan Gotić (mentor)
MentorInga Marijanović (mentor)
Committee memberInga Marijanović (predsjednik povjerenstva)
Committee memberNenad Judaš (član povjerenstva)
Committee memberIva Juranović Cindrić (član povjerenstva)
Committee memberTomislav Jednačak (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2017-07-18, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Nanočestice zlata (AuNČ) koriste se kao biosenzori i kao kontrasni i radiosenzitizacijski agensi. Pored primjene u biomedicini, AuNČ pokazuju i izrazitu katalitičku aktivnost za oksidaciju alkohola. U ovom diplomskom radu AuNČ su sintetizirane novim originalnim postupkom oksidacije alkohola kod alkalnog pH. Sintetizirane AuNČ analizirane su pretražnom elektronskom mikroskopijom i rentgenskom difrakcijom. Koncentracija nanočestica u alkoholnim suspenzijama određena je uređajem za analizu praćenja nanočestica (engl. Nanoparticle tracking analysis). Sintetizirane AuNČ aplicirane su na stanice HEK 293 (engl. human embrionic kidney cells 293) u koncentracijskom rasponu od 0,88 x 109 do 1,80 x 1011 NČ L-1, a vijabilnost stanica ispitana je testom MTT. Vijabilnost stanica HEK 293 24 sata nakon izlaganja uzorcima AuNČ dispergiranih u alkoholu je bila veća od vijabilnosti stanica izlaganih samo pripadajućem alkoholu. Nakon 48 sati izlaganja stanica HEK 293 AuNČ, vijabilnost stanica relativno je nešto manja u odnosu na iste uvjete kao kod 24 sati. Benzilni alkohol ima aromatski karakter, a n-propanol i 1-pentanol su lančaste molekule što može biti razlog za toksični utjecaj benzilnog alkohola na stanice.
Abstract (english)
Gold Nanoparticles (AuNPs) are used as biosensors and as contrast and radiosensitizing agents. In addition to biomedical applications, AuNPs have shown excellent catalytic activity for alcohol oxidation. In this thesis, AuNPs were synthesized using new synthesis route, i.e. by alcohol oxidation in solution at alkaline pH. Synthesized AuNPs were examined using scanning electron microscope and X-ray diffraction. Besides, the synthesized AuNPs were applied to HEK 293 (human embryonic kidney cells 293) cells in concentration range between 0,88 x 109 and 1,80 x 1011 NP L-1 and the viability of cells was examined using MTT test. Viability of HEK 293 cells 24 hours after being exposed to AuNPs dispersed in alcohol was higher compared to viability of cells exposed only to corresponding alcohol. After 48 hours exposure of cells to AuNPs viability of cells was lower in comparison to the 24 hours exposure under the same experimental conditions. Since benzyl alcohol has aromatic character, whereas n-propanol and 1-pentanol are chained molecules, this fact may be a reason for toxic effect of benzyl alcohol on HEK 293 cells.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:174730
Study programmeTitle: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-09-07 10:03:02