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undergraduate thesis
Provjera kontrole kvalitete reakcije aminoaciliranja
Zagreb: University of Zagreb, Faculty of Science, 2017. urn:nbn:hr:217:629663
Stojan, Iva
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Stojan, I. (2017). Provjera kontrole kvalitete reakcije aminoaciliranja (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:629663

Stojan, Iva. "Provjera kontrole kvalitete reakcije aminoaciliranja." Undergraduate thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:629663

Stojan, Iva. "Provjera kontrole kvalitete reakcije aminoaciliranja." Undergraduate thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:629663

Stojan, I. (2017). 'Provjera kontrole kvalitete reakcije aminoaciliranja', Undergraduate thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:629663

Stojan I. Provjera kontrole kvalitete reakcije aminoaciliranja [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Science; 2017 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:629663

I. Stojan, "Provjera kontrole kvalitete reakcije aminoaciliranja", Undergraduate thesis, University of Zagreb, Faculty of Science, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:217:629663

Metadata
TitleProvjera kontrole kvalitete reakcije aminoaciliranja
Title (english)Quality control in the reaction of aminoacylation
AuthorIva Stojan
MentorIta Gruić Sovulj (mentor)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2017-09-08, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Aminoacil-tRNA-sintetaze (aaRS) su enzimi, prevoditelji genetičkog koda bez kojih proces translacije, pa ni sam život kakav danas poznajemo, ne bi postojao. Omogućavaju pravilno sparivanje aminokiselina i pripadnih molekula tRNA te doprinose visokoj točnosti procesa translacije. Provode raznovrsne mehanizme provjere kontrole kvalitete reakcije aminoaciliranja uključujući i hidrolitičke mehanizme popravka pogrešno aktiviranih aminokiselina i aminoaciliranih tRNA. Važnost popravka pogreške mehanizmom poslije prijenosa aminokiseline na molekulu tRNA (prema engl. posttransfer editing) kojeg provode ovi moćni enzimi pomoću svojih dodatnih domena za popravak, uočava se na primjeru norvalina i meta-tirozina. Popravak pogreške važan je za sprječavanje pogrešne aktivacije i prijenosa nepripadnih proteinogenih aminokiselina na tRNA, ali u zadnje vrijeme se uočava važnost i kod pogrešne aktivacije i prijenosa neproteinogenih aminokiselina na tRNA. Norvalin i meta-tirozin su neproteinogene aminokiseline, analozi leucina i fenilalanina koji predstavljaju veliku prijetnju točnosti procesa translacije i staničnom preživljavanju u uvjetima stresa pogotovo u kompetitivnom okruženju. Mehanizmi popravka pogreške poslije prijenosa efikasno eliminiraju navedene aminokiseline iz genetičkog koda. Proučavanjem mehanizama popravka pogrešaka koje provode aaRS, a koji nisu uvijek i posve evolucijski očuvani, otvara se put razvitku novih antibiotika kojima su bakterijske sintetaze mete, te se omogućava bolje razumijevanje određenih bolesti i razvitak njihovih boljih tretmana.
Abstract (english)
Aminoacyl-tRNA synthetases (aaRS) are enzymes, translators of the genetic code, without which the proces of translation, as well as life as we know it, would not exist. They precisely couple amino acids and cognate tRNA molecules, ensuring in that way a high-fidelity translation. They provide powerful mechanisms of aminoacylation quality control as well as hydrolitic editing (proofreading) of misactivated amino acids and misacylated tRNAs. The importance of posttransfer editing, which takes place within the specific editing domains of aaRS, is obvious in examples of norvaline and meta-tyrosine. Proofreading is of great significance for preventing misactivation and transfer of non-cognate proteinogenic amino acids to tRNAs, but lately its importance in preventing misactivation and transfer of non-proteinogenic amino acids to tRNAs has been noticed. Norvaline and meta-tyrosine are non-proteinogenic amino acids, analogues of leucine and phenylalanine respectively, which pose a significant threat to the accuracy of translation and celullar fitness. Posttransfer editing eliminates effectively both amino acids from the genetic code. Better understanding of the mechanisms of aaRS proofreading opens the door to further development of new antibiotics that target bacterial enzymes, ensures more detailed insight in specific diseases and provides new and possibly more successful medical treatments.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:629663
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-09-29 12:03:35