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master's thesis
Učinkovitost 5-fluorouracila i biološki aktivnih tvari iz pripravka gljiva "Agarikon plus" na rast tumora i angiogenezu u miša
Zagreb: University of Zagreb, Faculty of Science, 2017. urn:nbn:hr:217:840757
Jagečić, Denis
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Jagečić, D. (2017). Učinkovitost 5-fluorouracila i biološki aktivnih tvari iz pripravka gljiva "Agarikon plus" na rast tumora i angiogenezu u miša (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:840757

Jagečić, Denis. "Učinkovitost 5-fluorouracila i biološki aktivnih tvari iz pripravka gljiva "Agarikon plus" na rast tumora i angiogenezu u miša." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:840757

Jagečić, Denis. "Učinkovitost 5-fluorouracila i biološki aktivnih tvari iz pripravka gljiva "Agarikon plus" na rast tumora i angiogenezu u miša." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:840757

Jagečić, D. (2017). 'Učinkovitost 5-fluorouracila i biološki aktivnih tvari iz pripravka gljiva "Agarikon plus" na rast tumora i angiogenezu u miša', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:840757

Jagečić D. Učinkovitost 5-fluorouracila i biološki aktivnih tvari iz pripravka gljiva "Agarikon plus" na rast tumora i angiogenezu u miša [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2017 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:840757

D. Jagečić, "Učinkovitost 5-fluorouracila i biološki aktivnih tvari iz pripravka gljiva "Agarikon plus" na rast tumora i angiogenezu u miša", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:217:840757

Metadata
TitleUčinkovitost 5-fluorouracila i biološki aktivnih tvari iz pripravka gljiva "Agarikon plus" na rast tumora i angiogenezu u miša
Title (english)Efficacy of biologically active components from mushroom preparations "Agarikon plus" and 5-fluorouracil on tumor growth and angiogenesis in mice
AuthorDenis Jagečić
MentorNada Oršolić (mentor)
Committee memberNada Oršolić (predsjednik povjerenstva)
Committee memberSandra Radić Brkanac (član povjerenstva)
Committee memberJasna Lajtner (član povjerenstva)
Committee memberAna Galov (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2017-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Različite mutacije, genetske i epigenetske promjene uzrokuju nastanak tumora. Povećana aktivnost faktora rasta VEGF, matriks metaloproteinaza (MMP), dušičnog oksida (NO), te oslabljeni imunosni nadzor ostvaren inhibicijom M1 makrofaga i aktivacijom M2 makrofaga doprinose rastu, razvoju i napredku tumora. Produkti medicinskih gljiva imaju razvijene imunomodulacijske, hepatoprotektivne, antidijabetske, antibakterijske, antivirusne i antioksidativne mehanizme kojima usporavaju i zaustavljaju tumorski rast i progresiju. U ovom radu istražen je pojedinačan učinak komercijalnog pripravka Agarikon plus, sastavljenog od ekstrakta medicinskih gljiva, te njegov kombinirani učinak s kemoterapeutikom 5- Fluorouracilom (5-FU) na smanjenje tumorskog rasta, progresiju, metastaziranje, te stimulaciju protutumorske imunosti. U pokusu su korištene maligne stanice CT26.WT karcinoma kolona koje su bile unesene u miševe soja BALB/c. Sedmog dana od injiciranja CT26.WT stanica, miševi su pomoću gastrične kanile dobivali Agarikon plus tijekom četrnaest dana, sa ili bez 5-FU. Životinje su žrtvovane 36. dana. Dobiveni rezultati ukazuju da Agarikon plus sam i/ili u kombinaciji s 5-FU smanjuje volumen tumora, angiogenezu i mikrožilnu gustoću, potvrđujući anti-angiogeni potencijal pripravka Agarikon plus in vivo. Kombinacija pripravka Agarikon plus i 5-FU te sam 5-FU pokazuju povećanu razinu NO u makrofazima što dovodi do aktivacije makrofaga, aktivacije Th1 imunosnog odgovora te povećanja citotoksičnog učinka na stanice tumora, inhibicije angiogeneze i rasta tumora.
Abstract (english)
Various mutations, genetic and epigenetic changes are the cause of tumor formation. Increased activity of the VEGF factor, matrix metalloproteinase (MMP), nitric oxide (NO), and the impaired immune monitoring by inhibition of M1 macrophage and activation of M2 macrophages contribute to growth, development and progression of tumors. Medicinal fungi products have developed immunomodulatory, hepatoprotective, anti-diabetic, antibacterial, antiviral and antioxidant mechanisms that slow down and stop tumor growth and progression. This paper explored the singular effect of the commercially available Agarikon plus medicament extract, and its combined effect with 5-fluorouracil (5-FU) chemotherapeutic on decreasing tumor growth, progression, metastasis, and stimulation of anti-inflammatory immunity. In the experiment, CT26.WT colon cancer cells were injected into the BALB/c mice. On the seventh day of inoculation of CT26.WT cells, mice were given Agarikon plus by gastric cannula for fourteen days, with or without 5-FU. Animals are sacrificed on the 36th day. The obtained results indicate that Agarikon plus alone and/or in combination with 5-FU reduces tumor volume, angiogenesis and microarray density, confirming the anti-angiogenic potency of Agarikon plus in vivo. The combination of Agarikon plus and 5-FU and 5-FU alone show increased levels of NO in macrophages, leading to macrophage activation, activation of Th1 immune response and increased cytotoxic effect on tumor cells, inhibition of angiogenesis, and tumor growth.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:840757
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-10-20 09:43:50