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master's thesis
Usporedba razine serotonina u mozgu gušterica vrsta Podarcis siculus (Rafinesque-Schmaltz, 1810) i Podarcis melisellensis (Braun, 1877)
Zagreb: University of Zagreb, Faculty of Science, 2017. urn:nbn:hr:217:042926
Burić, Davorka
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Burić, D. (2017). Usporedba razine serotonina u mozgu gušterica vrsta Podarcis siculus (Rafinesque-Schmaltz, 1810) i Podarcis melisellensis (Braun, 1877) (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:042926

Burić, Davorka. "Usporedba razine serotonina u mozgu gušterica vrsta Podarcis siculus (Rafinesque-Schmaltz, 1810) i Podarcis melisellensis (Braun, 1877)." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:042926

Burić, Davorka. "Usporedba razine serotonina u mozgu gušterica vrsta Podarcis siculus (Rafinesque-Schmaltz, 1810) i Podarcis melisellensis (Braun, 1877)." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:042926

Burić, D. (2017). 'Usporedba razine serotonina u mozgu gušterica vrsta Podarcis siculus (Rafinesque-Schmaltz, 1810) i Podarcis melisellensis (Braun, 1877)', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:042926

Burić D. Usporedba razine serotonina u mozgu gušterica vrsta Podarcis siculus (Rafinesque-Schmaltz, 1810) i Podarcis melisellensis (Braun, 1877) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2017 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:042926

D. Burić, "Usporedba razine serotonina u mozgu gušterica vrsta Podarcis siculus (Rafinesque-Schmaltz, 1810) i Podarcis melisellensis (Braun, 1877)", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:217:042926

Metadata
TitleUsporedba razine serotonina u mozgu gušterica vrsta Podarcis siculus (Rafinesque-Schmaltz, 1810) i Podarcis melisellensis (Braun, 1877)
Title (english)Comparison of serotonin levels in the brains of lizard species Podarcis siculus (Rafinesque-Schmaltz, 1810) and Podarcis melisellensis (Braun, 1877)
AuthorDavorka Burić
MentorDubravka Hranilović (mentor)
Committee memberDubravka Hranilović (predsjednik povjerenstva)
Committee memberBiljana Balen (član povjerenstva)
Committee memberRenata Matoničkin Kepčija (član povjerenstva)
Committee memberSofia Ana Blažević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2017-09-18, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Podarcis melisellensis (krška gušterica) i Podarcis siculus (primorska gušterica) srodne su gušterice iz porodice Lacertidae. Terenskim i laboratorijskim istraživanjima pokazano je da P. siculus kompeticijom istiskuje P. melisellensis, osobito na manjim otocima. Uz razliku u veličini i načinu prehrane, jedan od razloga ugroženosti krške gušterice mogla bi biti i razlika u ponašanju, koje je prvenstveno regulirano monoaminskim neurotransmitorskim sustavima. Cilj ovog diplomskog rada bio je prilagoditi postojeću metodu mjerenja razine serotonina u mozgu štakora HPLC-om za mjerenje u mozgu gušterice te istražiti postoji li razlika u koncentraciji serotonina između ove dvije vrste, kao neurobiološka osnova ranije opaženih razlika u ponašanju. Metoda je uspješno prilagođena za mjerenja u mozgu gušterice pri čemu optimalni pH vodene faze iznosi 2,8, a injekcijski volumen uzorka 30 L, dok je linearnost i selektivnost metode, kao i ponovljivost unutar dana zadovoljavajuća. Koncentracije serotonina u mozgu gušterica P. melisellensis i P. siculus, nisu se statistički značajno razlikovale, a razlika nije uočena ni između spolova. Stoga pretpostavljamo da su ranije opažene razlike u ponašanju prvenstveno rezultat razlike u razini katekolamina u mozgu ovih dviju vrsta gušterica.
Abstract (english)
Podarcis melisellensis (Dalmatian wall lizard) and Podarcis siculus (Italian wall lizard) are related lizard species of family Lacertidae. Field and laboratory research have demonstrated that P. melisellensis is being competitively excluded by P. siculus, especially on small islands. Besides differences in size and diet, one of the reasons for the endangerment of P. melisellensis might be a difference in behavior, which is primarily regulated through monoamine neurotransmitter systems. The aim of this thesis was adaptation of the current method for measurement of serotonin concentrations in the rat brain by HPLC for measurements in the lizard brain, and search for the potential difference in serotonin concentrations as a possible neurobiological background of the observed differences in behavior. The method was successfully adapted for measurements in the lizard brain with optimal pH of 2,8, and injection volume of the sample of 30 L. Linearity and selectivity of the method, as well as daily reproducibility was satisfactory. Serotonin concentrations in the brains of P. melisellensis i P. siculus, did not significantly differ. Gender difference was also not significant. We can therefore assume that previously observed differences in behavior might be induced primarily by differences in catecholamine levels in the brains of the two lizard species.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:042926
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2018-09-18
Terms of useLicence In copyright icon
Created on2017-10-20 11:56:10