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master's thesis
Kinetička analiza heterocikličkih α-D-manopiranozida kao potencijalnih inhibitora butirilkolinesteraze
Zagreb: University of Zagreb, Faculty of Science, 2017. urn:nbn:hr:217:235664
Poletar, Matej
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Poletar, M. (2017). Kinetička analiza heterocikličkih α-D-manopiranozida kao potencijalnih inhibitora butirilkolinesteraze (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:235664

Poletar, Matej. "Kinetička analiza heterocikličkih α-D-manopiranozida kao potencijalnih inhibitora butirilkolinesteraze." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:235664

Poletar, Matej. "Kinetička analiza heterocikličkih α-D-manopiranozida kao potencijalnih inhibitora butirilkolinesteraze." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:235664

Poletar, M. (2017). 'Kinetička analiza heterocikličkih α-D-manopiranozida kao potencijalnih inhibitora butirilkolinesteraze', Master's thesis, University of Zagreb, Faculty of Science, accessed 28 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:235664

Poletar M. Kinetička analiza heterocikličkih α-D-manopiranozida kao potencijalnih inhibitora butirilkolinesteraze [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2017 [cited 2024 December 28] Available at: https://urn.nsk.hr/urn:nbn:hr:217:235664

M. Poletar, "Kinetička analiza heterocikličkih α-D-manopiranozida kao potencijalnih inhibitora butirilkolinesteraze", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:217:235664

Metadata
TitleKinetička analiza heterocikličkih α-D-manopiranozida kao potencijalnih inhibitora butirilkolinesteraze
Title (english)Kinetic analysis of heterocyclic α-D-mannopiranosides as potential inhibitors of butyrylcholinesterase
AuthorMatej Poletar
MentorSrđanka Tomić-Pisarović (mentor)
Committee memberSrđanka Tomić-Pisarović (predsjednik povjerenstva)
Committee memberMarko Močibob (član povjerenstva)
Committee memberSnežana Miljanić (član povjerenstva)
Committee memberVesna Petrović Peroković (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2017-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
U okviru ovog diplomskog rada ispitana su inhibitorna svojstva 11 novih heterocikličkih α-D-manopiranozida. Aktivnost liofilizirane butirilkolinesteraze izolirane iz konjskog seruma (EqBChE, akt. 0,08 U/mL) određena je spektrofotometrijskom metodom po Ellmanu. Mjerenja aktivnosti su vršena uz acetiltiokolin-jodid kao supstrat (u konc. rasponu 0,04 – 1 mM), tiolni reagens DTNB (0,3 mM) i spojeve 1 – 11 (10 – 300 μM). Povećanje apsorbancije TNB aniona praćeno je u kvarcnim kivetama (ukupni volumen reakcijske smjese 2 mL) u 0,1 M fosfatnom puferu (pH 7,4) pri 25 oC i valnoj duljini 412 nm kroz 90 sekundi na spektrofotometru AnalytikJena Specord 200. Od jedanaest ispitanih spojeva, šest je pokazalo inhibitornu aktivnost prema EqBChE, od kojih su dva relativno dobri inhibitori s konstantama inhibicije 12,40±1,226 μM (za spoj 7) i 37,28±3,982 μM (za spoj 4).
Abstract (english)
The aim of this thesis is to investigate inhibitory properties of 11 newly synthesized heterocyclic α-D-mannopiranosides towards BChE. Ellman's spectrophotometric method was used to determine activities of lyophilized butyrylcholinesterase from equine serum (EqBChE, act. 0,08 U/mL). Measurments were conducted with acetylthiocholine iodide as a supstrate (conc. range 0,04 – 1 mM), thiol reagent DTNB (0,3 mM) and compounds 1 -11 (10 – 300 μM). Increase in TNB anion absorbance was monitored in quartz cuvettes (total volume 2 mL) in 0,1 M phosphate buffer medium (pH 7,4) at 25 oC and wavelength 412 nm during 90 seconds on AnalytikJena Specord 200 spectrophotometer. Six out of eleven tested compounds have shown inhibitory effect on BChE and two of them are relatively good BChE inhibitors with constants of inhibition 12,40±1,226 μM (compound 7) i 37,28±3,982 μM (compound 4).
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:235664
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2019-09-28
Terms of useLicence In copyright icon
Created on2017-10-25 10:06:43