prikaz prve stranice dokumenta Biokemijske i epigenetičke promjene u gljive Cryphonectria parasitica (Murr.) Barr zaražene virusom Cryphonectria hypovirus 1
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doctoral thesis
Biokemijske i epigenetičke promjene u gljive Cryphonectria parasitica (Murr.) Barr zaražene virusom Cryphonectria hypovirus 1
Zagreb: University of Zagreb, Faculty of Science, 2017. urn:nbn:hr:217:705728
Nuskern, Lucija
University of Zagreb
Faculty of Science

Institutional repository: Repository of the Faculty of Science

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Nuskern, L. (2017). Biokemijske i epigenetičke promjene u gljive Cryphonectria parasitica (Murr.) Barr zaražene virusom Cryphonectria hypovirus 1 (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:705728

Nuskern, Lucija. "Biokemijske i epigenetičke promjene u gljive Cryphonectria parasitica (Murr.) Barr zaražene virusom Cryphonectria hypovirus 1." Doctoral thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:705728

Nuskern, Lucija. "Biokemijske i epigenetičke promjene u gljive Cryphonectria parasitica (Murr.) Barr zaražene virusom Cryphonectria hypovirus 1." Doctoral thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:705728

Nuskern, L. (2017). 'Biokemijske i epigenetičke promjene u gljive Cryphonectria parasitica (Murr.) Barr zaražene virusom Cryphonectria hypovirus 1', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:705728

Nuskern L. Biokemijske i epigenetičke promjene u gljive Cryphonectria parasitica (Murr.) Barr zaražene virusom Cryphonectria hypovirus 1 [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2017 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:705728

L. Nuskern, "Biokemijske i epigenetičke promjene u gljive Cryphonectria parasitica (Murr.) Barr zaražene virusom Cryphonectria hypovirus 1", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:217:705728

Metadata
TitleBiokemijske i epigenetičke promjene u gljive Cryphonectria parasitica (Murr.) Barr zaražene virusom Cryphonectria hypovirus 1
Title (english)Biochemical and epigenetic changes in Cryphonectria hypovirus 1- infected fungus Cryphonectria parasitica (Murr.) Barr
AuthorLucija Nuskern
MentorMirna Ćurković Perica (mentor)
Committee memberMirta Tkalec (predsjednik povjerenstva)
Committee memberMartina Šeruga Musić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
Zagreb
Defense date and country2017, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		57 - Biological sciences. Physical anthropology. Biodiversity
Abstract
S ciljem određivanja učinka infekcije virusom Cryphonectria hypovirus 1 (CHV1) na intenzitet promjena u metabolizmu gljive Cryphonectria parasitica, u tri virulentna izolata gljive uneseno je šest različitih sojeva virusa talijanskog (I), francuskog (F1) i rekombiniranog talijansko-francuskog (F1/I) podtipa. Infekcija virusom CHV1 dovela je do i) indukcije antioksidacijske obrane u zaraženoj gljivi, odnosno do povećanja aktivnosti antioksidacijskih enzima glutation S-transferaze, katalaze i superoksid dismutaze, ii) do porasta aktivnosti lakaza zaražene gljive, iii) do epigenetičkih promjena, odnosno do porasta metiliranosti CpG sekvenci genoma gljive. Postavljena je hipoteza da je povećana metilacija genoma gljive C. parasitica nakon infekcije virusom čimbenik virulentnosti virusa, a ne dio obrambenog mehanizma gljive. Svi istraživani parametri pokazali su da intenzitet promjena u metabolizmu gljive ovisi o virusnom soju, a ne podtipu virusa, te o genotipu gljive domaćina i kombinaciji genoma gljive i virusa, te je naglašena važnost korištenja više virusnih sojeva i izolata gljive u pokušajima biološke kontrole.
Abstract (english)
Six different strains of Cryphonectria hypovirus 1 (CHV1), of Italian (I), French (F1) and recombinant Italian-French (F1/I) subtype, were transferred into three virulent isolates of fungus Cryphonectria parasitica to determine the effect of virus infection on the fungal metabolism. CHV1 infection: i) induced antioxidative defence in infected fungus – increased activities of antioxidative enzymes glutathione S-transferase, catalase and superoxide dismutase, ii) increased activity of fungal laccases, iii) induced epigenetic changes in the genome of infected fungus – increased methylation levels at CpG sites. Methylation of C. parasitica genome upon CHV1 infection seems to be a virulence determinant of the virus rather than a part of defensive mechanism of the fungus. The severity of observed effects did not depend on virus subtype, but rather on a particular virus strain, fungal genotype, and the combination of the two, stressing the importance of using more virus strains and fungal isolates in the biocontrol efforts.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:705728
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-11-02 11:46:43