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master's thesis
Identifikacija motornih proteina u premosnom vlaknu mitotskog diobenog vretena
Zagreb: University of Zagreb, Faculty of Science, 2017. urn:nbn:hr:217:456934
Risteski, Patrik
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Risteski, P. (2017). Identifikacija motornih proteina u premosnom vlaknu mitotskog diobenog vretena (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:456934

Risteski, Patrik. "Identifikacija motornih proteina u premosnom vlaknu mitotskog diobenog vretena." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:456934

Risteski, Patrik. "Identifikacija motornih proteina u premosnom vlaknu mitotskog diobenog vretena." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:456934

Risteski, P. (2017). 'Identifikacija motornih proteina u premosnom vlaknu mitotskog diobenog vretena', Master's thesis, University of Zagreb, Faculty of Science, accessed 03 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:456934

Risteski P. Identifikacija motornih proteina u premosnom vlaknu mitotskog diobenog vretena [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2017 [cited 2024 May 03] Available at: https://urn.nsk.hr/urn:nbn:hr:217:456934

P. Risteski, "Identifikacija motornih proteina u premosnom vlaknu mitotskog diobenog vretena", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:217:456934

Metadata
TitleIdentifikacija motornih proteina u premosnom vlaknu mitotskog diobenog vretena
Title (english)Identification of motor proteins in the bridging fiber of the mitotic spindle
AuthorPatrik Risteski
MentorIva Tolić (mentor)
MentorBiljana Balen (mentor)
Committee memberBiljana Balen (predsjednik povjerenstva)
Committee memberMartina Šeruga Musić (član povjerenstva)
Committee memberDuje Lisičić (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2017-11-16, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Na početku mitoze, stanica konstruira diobeno vreteno, dinamičnu strukturu odgovornu za preciznu segregaciju repliciranog genoma između stanica kćeri. Ova jedinstvena citoskeletna struktura sastavljena je od kromosoma, mikrotubula i proteina povezanih s mikrotubulima. Nedavna istraživanja su započela s otkrivanjem nove grupe mikrotubula, nazvanih mikrotubuli premosnice, koji premošćuju regiju između sestrinskih kinetohora i pritom tvore premosno vlakno, lateralno povezano sa sestrinskim k-vlaknima. U ovom radu, identificiran je položaj motornih proteina povezanih s mikrotubulima u mitotskom diobenom vretenu stanica HeLa u odnosu na pozicije premosnih vlakana. Protein MKLP1, motor kinezin-6, pronađen je kako obogaćuje centralni dio diobenog vretena na pozicijama premosnih vlakana. Prekomjerna ekspresija proteina PRC1, antiparalelnog veznika koji je potvrđen u premosnom vlaknu, pokazala je korelaciju između lokalizacije proteina MKLP1 i premosnih preklapajućih regija označenih s proteinom PRC1. Nadalje, utišavanje Kif4A, poznatog veznog partnera proteina PRC1, uzrokuje elongaciju preklapajućih regija premosnih vlakana.
Abstract (english)
At the onset of mitosis, cell constructs a spindle, a dynamic structure responsible for precise segregation of replicated genome between daughter cells. This unique cytoskeletal apparatus is made of chromosomes, microtubules and microtubule-associated proteins. Recent studies have begun to uncover a new class of microtubules, termed bridging microtubules, that span the region between sister kinetochores, whilst forming the bridging fiber that laterally connects sister k-fibers. In this thesis, I have identified localization of motor microtubule-associated proteins in the mitotic spindle of HeLa cells with respect to the bridging fiber positions. MKLP1, a kinesin-6 motor, was observed to enrich the central part of the spindle at the positions of the bridging fibers. Overexpression of PRC1, an anti-parallel cross-linker, which is confirmed in the bridging fibers, shows correlation of MKLP1 localization with PRC1-labeled bridging overlaps. Additionally, silencing of Kif4A, a major binding partner of PRC1, elongates overlap regions of the bridging fibers.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:456934
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2019-11-16
Terms of useLicence In copyright icon
Created on2017-12-21 08:42:39