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undergraduate thesis
Transport rekombinantnih proteina sa signalom za zadržavanje u endoplazmatskom retikulumu (ER) u biljci uročnjak (Arabidopsis thaliana)
Zagreb: University of Zagreb, Faculty of Science, 2016. urn:nbn:hr:217:105315
Tudić, Antonio
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Tudić, A. (2016). Transport rekombinantnih proteina sa signalom za zadržavanje u endoplazmatskom retikulumu (ER) u biljci uročnjak (Arabidopsis thaliana) (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:105315

Tudić, Antonio. "Transport rekombinantnih proteina sa signalom za zadržavanje u endoplazmatskom retikulumu (ER) u biljci uročnjak (Arabidopsis thaliana)." Undergraduate thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:105315

Tudić, Antonio. "Transport rekombinantnih proteina sa signalom za zadržavanje u endoplazmatskom retikulumu (ER) u biljci uročnjak (Arabidopsis thaliana)." Undergraduate thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:105315

Tudić, A. (2016). 'Transport rekombinantnih proteina sa signalom za zadržavanje u endoplazmatskom retikulumu (ER) u biljci uročnjak (Arabidopsis thaliana)', Undergraduate thesis, University of Zagreb, Faculty of Science, accessed 19 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:105315

Tudić A. Transport rekombinantnih proteina sa signalom za zadržavanje u endoplazmatskom retikulumu (ER) u biljci uročnjak (Arabidopsis thaliana) [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Science; 2016 [cited 2024 May 19] Available at: https://urn.nsk.hr/urn:nbn:hr:217:105315

A. Tudić, "Transport rekombinantnih proteina sa signalom za zadržavanje u endoplazmatskom retikulumu (ER) u biljci uročnjak (Arabidopsis thaliana)", Undergraduate thesis, University of Zagreb, Faculty of Science, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:217:105315

Metadata
TitleTransport rekombinantnih proteina sa signalom za zadržavanje u endoplazmatskom retikulumu (ER) u biljci uročnjak (Arabidopsis thaliana)
Title (english)Trafficking of endoplasmic reticulum (ER)-retained recombinant proteins in Arabidopsis thaliana
AuthorAntonio Tudić
MentorBiljana Balen (mentor)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2016, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Mnogi rekombinantni proteini se često proizvode u eukariotskim organizmima, kao što je A. thaliana, kako bi se osiguralo pravilno smatanje, formacija disulfidnih mostova i procesiranje N-glikana. Proteini se kotranslacijski prenose u endoplazmatski retikulum (ER), nakon čega se zadržavaju unutar njega ili se transportiraju u različite unutarstanične odjeljke ili u izvanstanični prostor. Razni su molekularni signali potrebni za zadržavanje proteina unutar ER-a ili prijenos u različite odjeljke. Dodavanjem C-terminalnog signalnog slijeda H/KDEL, rekombinantni proteini mogu biti konstruirani za zadržavanje u ER-u. Unatoč velikom znanju o transportu proteina, konačna destinacija rekombinantnih proteina, posebno onih s oznakom H/KDEL, vrlo je nepredvidljiva. Kompleksni proteini sisavaca mogu se proizvesti u transformiranim biljkama ili transformiranim suspenzijama biljnih stanica. Molekularni uzgoj u biljkama ima potencijal za proizvodnju neograničenih količina rekombinantnih proteina koji se koriste kao dijagnostički i terapeutski alati u zdravstvu i znanosti. Potrebna su daljnja istraživanja koja bi pomogla u predviđanju zadržavanja prekomjerno eksprimiranih proteina sa signalom H/KDEL unutar ER-a u biljkama, te koja bi doprinijela daljnjem razvijanju molekularnog uzgoja biljaka.
Abstract (english)
Many recombinant proteins are often produced in eukaryotic host organisms, such as Arabidopsis, to ensure proper folding, disulfide bridge formation and N-glycan processing. Proteins are co-translationally transferred into the endoplasmic reticulum (ER) and then either retained or transported to different intracellular compartments or to the extracellular space. Various molecular signals necessary for retention in the ER or targeting to different compartments have been identified. Recombinant proteins can be designed for ER retention by adding a C-terminal H/KDEL-tag. Despite extensive knowledge of the protein trafficking pathways, the final protein destination, especially of such H/KDEL-tagged recombinant proteins, is unpredictable. Complex mammalian proteins can be produced in transformed plants or transformed plant suspension cells. Molecular farming in plants has the potential to provide unlimited quantities of recombinant proteins for use as diagnostic and therapeutic tools in health care and the life sciences. Further investigations will result in a much better predictability of the ER retention of overexpressed H/KDEL-tagged proteins in plants, and eventually contribute to the further establishment of the field of plant molecular farming.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:105315
Study programmeTitle: Ecology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2018-01-04 11:56:16