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master's thesis
Ekspresija gena za biljnu seril-tRNA-sintetazu u uvjetima stresa
Zagreb: University of Zagreb, Faculty of Science, 2017. urn:nbn:hr:217:745477
Baranašić, Jurica
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Baranašić, J. (2017). Ekspresija gena za biljnu seril-tRNA-sintetazu u uvjetima stresa (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:745477

Baranašić, Jurica. "Ekspresija gena za biljnu seril-tRNA-sintetazu u uvjetima stresa." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:745477

Baranašić, Jurica. "Ekspresija gena za biljnu seril-tRNA-sintetazu u uvjetima stresa." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:745477

Baranašić, J. (2017). 'Ekspresija gena za biljnu seril-tRNA-sintetazu u uvjetima stresa', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:745477

Baranašić J. Ekspresija gena za biljnu seril-tRNA-sintetazu u uvjetima stresa [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2017 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:745477

J. Baranašić, "Ekspresija gena za biljnu seril-tRNA-sintetazu u uvjetima stresa", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:217:745477

Metadata
TitleEkspresija gena za biljnu seril-tRNA-sintetazu u uvjetima stresa
Title (english)Expression of the plant-seryl-tRNA synthetase gene under stress conditions
AuthorJurica Baranašić
MentorJasmina Rokov Plavec (mentor)
Committee memberJasmina Rokov Plavec (predsjednik povjerenstva)
Committee memberIva Juranović Cindrić (član povjerenstva)
Committee memberBiserka Prugovečki (član povjerenstva)
Committee memberMarko Močibob (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2017-02-09, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Seril-tRNA-sintetaza (SerRS) kao i ostale aminoacil-tRNA-sintetaze (aaRS) kovalentno povezuje aminokiselinu s odgovarajućom tRNA. Uz kanonsku ulogu u biosintezi proteina uočene su i neke nekanonske uloge aaRS, kao npr. sudjelovanje u staničnom odgovoru na stres. Prethodno je uočeno da transgenične biljke Arabidopsis thaliana koje prekomjerno eksprimiraju SerRS bolje kliju i brže rastu u odnosu na divlji tip biljke nakon izlaganja stresu. Također je pokazano kako SerRS stupa u interakciju s proteinom BEN1 koji sudjeluje u metabolizmu biljnih hormona brasinosteroida, koji sudjeluju u staničnom odgovoru na stres. U svrhu bolje karakterizacije interakcije SerRS i BEN1 te njihove potencijalne uloge u staničnom odgovoru na stres, analizirane su transgenične biljke koje prekomjerno eksprimiraju SerRS u obliku fuzijskih proteina. RT-qPCR-om, western-hibridizacijom, fluorescencijskom mikroskopijom i analizom enzimske aktivnosti pokazano je kako sve ispitane transgenične linije prekomjerno eksprimiraju kinetički aktivne fuzijske SerRS proteine. Fenotipskom analizom nije uočena razlika između transgeničnih biljaka i biljaka divljeg tipa. Prekomjerna ekspresija SerRS ne utječe na ekspresiju gena za BEN1 protein. Ispitana je ekspresija gena za SerRS pri četiri uvjeta abiotičkog stresa izazvanog povećanom koncentracijom kadmijevih iona, te je pokazano kako u većini uvjeta dolazi do povećanja ekspresije gena za SerRS. Podaci upućuju na aktivno sudjelovanje SerRS u staničnim odgovorima na abiotički stres.
Abstract (english)
Seryl-tRNA-synthetase (SerRS) just like all aminoacyl-tRNA synthetase covalently bonds amino acid with the cognate tRNA. Besides their main function in protein biosynthesis, aaRSa also developed some non-canonical functions such as participation in cellular responses to stress. Previously, it was shown that the transgenic Arabidopsis thaliana plants, that overexpress SerRS, sprout better and grow faster than wild type plants after the exposure to stress conditions. It was also shown that SerRS interacts with BEN1, a protein that is probably involved in cellular responses to stress. To better characterize the interaction between SerRS and BEN1 and their role in cell response to stress, transgenic lines that contain SerRS fusion constructs were investigated. RT-qPCR, western blot, enzyme activity analysis and fluorescent microscopy show that all transgenic lines overexpress kinetically active SerRS-fusion proteins. Phenotypic analysis didnt show any difference between transgenic plants and wild type plants. Overexpression of SerRS gene doesnt affect the expression of gene coding for BEN1. The expression of SerRS gene was monitored under four different conditions of abiotic stress, caused by high cadmium concentrations. The results show that under most conditions the expression of SerRS gene is enhanced, which indicates that SerRS is actively involved in cellular responses to abiotic stress.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:745477
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-02-20 10:52:39