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master's thesis
Procjena genotoksičnog učinka karbofurana u miša primjenom alkalnog komet-testa
Zagreb: University of Zagreb, Faculty of Science, 2009. urn:nbn:hr:217:564873
Bajić, Ana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Bajić, A. (2009). Procjena genotoksičnog učinka karbofurana u miša primjenom alkalnog komet-testa (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:564873

Bajić, Ana. "Procjena genotoksičnog učinka karbofurana u miša primjenom alkalnog komet-testa." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:564873

Bajić, Ana. "Procjena genotoksičnog učinka karbofurana u miša primjenom alkalnog komet-testa." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:564873

Bajić, A. (2009). 'Procjena genotoksičnog učinka karbofurana u miša primjenom alkalnog komet-testa', Master's thesis, University of Zagreb, Faculty of Science, accessed 26 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:564873

Bajić A. Procjena genotoksičnog učinka karbofurana u miša primjenom alkalnog komet-testa [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2009 [cited 2024 December 26] Available at: https://urn.nsk.hr/urn:nbn:hr:217:564873

A. Bajić, "Procjena genotoksičnog učinka karbofurana u miša primjenom alkalnog komet-testa", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2009. Available at: https://urn.nsk.hr/urn:nbn:hr:217:564873

Metadata
TitleProcjena genotoksičnog učinka karbofurana u miša primjenom alkalnog komet-testa
Title (english)Genotoxic carbofuran effect evaluation in mice by alkaline comet assay ussage
AuthorAna Bajić
MentorDavor Želježić (mentor)
MentorVesna Benković (sumentor)
Committee memberVesna Benković (predsjednik povjerenstva)
Committee memberDavor Želježić (član povjerenstva)
Committee memberMirjana Pavlica (član povjerenstva)
Committee memberRenata Šoštarić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2009, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Cilj istraživanja bio je istražiti genotoksični učinak insekticida karbofurana (2, 3-dihidro-2,2- dimetilbenzofuran-7-il-metilkarbamata) i njegove formulacije Geocid ST 35 (350g/l) primjenom alkalnog komet testa na stanicama organa soja miševa Swiss albino u uvjetima in vivo. Procjena genotoksičnosti provedena je u stanicama bubrega, jetre, koštane srži i leukocitima primjenom dvaju parametara komet testa: dužine repa i intenziteta repa. Karbofuranom i Geocidom ST 35, svakodnevno tijekom 14 dana, obrađivane su četiri skupine životinja s dozama aktivne tvari u vrijednostima ADI i 1/100LD50 i uspoređene s kontrolnom skupinom. Utvrđeno je znatno veće oštećenje DNA u stanicama svih organa skupina obrađenih formulacijom Geocidom ST 35 za vrijednosti ADI i 1/100LD50 u odnosu na stanice životinja obrađenih čistom aktivnom tvari karbofuran u istim dozama. I za aktivnu tvar i za formulaciju intenzitet oštećenja bio je proporcionalan dozi pesticida.U stanicama bubrega, jetre i koštane srži utvrđena su podjednaka oštećenja molecule DNA, dok je razina genomskim oštećenja u leukocitima bila nešto niža. Na temelju dobivenih rezultata može se zaključiti da karbofuran i formulacija Geocid ST 35 genotoksično djeluju na stanice svih ispitivanih organa miševa, ali ni karbofuran ni formulacija nisu pokazali specifičnost genotoksičnog učinka prema nijednom organu. Oštećenja utvrđena u stanicama jetre, bubrega i koštane srži, osim genotoksičnog djelovanja karbofurana rezultat su i fizioloških procesa u tim tkivima.
Abstract (english)
The aim of research was to explore the genotoxic impact of carbofouran pesticide (2,3-dihydro-2,2- dimethyl-7-benzofuranol methylcarbamate) and its formulation Geocid ST 35 (350g/l) by alkali comet assay usage on four organs´ cells of mice strain swiss albino in conditions in vivo. The genotoxic evaluation was made on kidney, liver, bone marrow cells and leukocytes using two comet assay parameters: tail length and tail intensity. During fourteen day period, four groups of animals were treated by carbofouran and Geocid ST 35 with an active substance doses in values of ADI i 1/100LD50 and were compared with the control group. Both active supstance and formulation have shown damage intensity proportional to pesticide doses. Kidney, liver and bone marrow cells have shown similar DNA damage while genom level was slightly lower in leukocytes. Based on given results it can be concluded that carbofuran and Geocid ST 35 have genotoxic effect on all treated murine organs, but niether carbofuran nor its formulation have shown specificy of genotoxic effect on niether of organs. Demage detected in all organ cells exept lukocytes is a result of both carbofuran genotoxity impact and physiological proceses in those tissues.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:564873
Study programmeTitle: Ecology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-03-06 09:14:18